Utilisation of tuna condensate waste from the canning industry as a novel substrate for polyhydroxyalkanoate production

  • Kanokphorn SangkharakEmail author
  • Nisa Paichid
  • Tewan Yunu
  • Sappasith Klomklao
  • Poonsuk Prasertsan
Original Article


Tuna condensate, an organic-rich by-product from the tuna canning industry, was assessed as a substrate for polyhydroxyalkanoate (PHA) production using Cupriavidus necator TISTR 1095. The effect of cultivation parameters on PHA accumulation was studied, including substrate concentration, carbon to nitrogen (C/N) ratio, initial pH-value control and fermentation strategies. For the bacterium, a biomass of 3.8 ± 0.1 g/L, PHA of 1.64 ± 0.1 g/L with PHA productivity of 0.027 g/L.h were obtained under batch cultivation using 100% tuna condensate with a C/N ratio of 88:1 and no control of pH. However, the PHA production was increased 1.3-fold when repeated-batch cultivation was applied. The highest biomass (7.5 ± 0.1 g/L) and PHA (3.8 ± 0.1 g/L) with 0.063 g/L.h of PHA productivity were achieved after the third cycle of repeated-batch cultivation. High chemical oxygen demand (COD) removal efficiency of 70% under the optimal condition was also demonstrated. The polymers generated by C. necator TISTR 1095 were characterised. The size of polymer granules was in the range of 0.7-0.8 μm. The polymer produced in the optimal medium under batch and repeated-batch cultivation was identified as poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with a 20 mol% of 3-hydroxyvalerate. The molecular mass (Mn) and polydispersity of the polymer were 2 × 106 Da and 2.5, respectively. The results demonstrated that tuna condensate could be used as a cheap substrate for PHA production on an industrial scale.


Cupriavidus necator 3-Hydroxybutyrate 3-Hydroxyvalerate Repeated-batch cultivation Tuna condensate 


Funding information

This work is supported by the Thailand Research Fund (TRF) Grant for Researcher (project number RSA 6180066 and RTA 6080010) and the Research and Development Institute at Thaksin University.


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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Chemistry, Faculty of ScienceThaksin UniversityPhatthalungThailand
  2. 2.Department of Food Science and Technology, Faculty of Agro and Bio IndustryThaksin UniversityPhatthalungThailand
  3. 3.Research and Development OfficePrince of Songkla UniversitySongkhlaThailand

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