Impact of bubble size on docosahexaenoic acid production by Crypthecodinium cohnii in bubble column bioreactor

  • Niloofar Hoseinkhani
  • Hasan JaliliEmail author
  • Shahin Ansari
  • Abdeltif Amrane
Original Article


Cultivation conditions especially agitation and aeration have critical roles on Crypthecodinium cohnii growth and docosahexaenoic acid (DHA) formation. In this study, for the first time, different air bubble sizes were considered, in a 5-L bubble column bioreactor; and the effect of bubble size on growth and on DHA production by dinoflagellate microalgae C. cohnii was investigated. The cultivation was carried out under 0.5 vvm aeration flow rate, 28 °C, and pH ± 7. The highest yield of DHA (1.4 g DHA L−1) was achieved for 0.36-cm air bubble diameter; the corresponding biomass amount was 17.6 g. The suitable conditions during the logarithmic stage of growth were provided for 0.36-cm air bubble diameter, while oxygen limitation was observed in the culture medium afterwards. The concentration of the microalgal DHA was increased in oxygen limitation conditions. Our results indicate that the air bubble diameter is the most influencing factor on DHA production in a bubble column bioreactor. Current research indicates that optimum bubble size in bubble column bioreactor can lead to optimal conditions for growth and DHA production like two-stage oxygen supply feeding strategy.


Bubble size Bubble column bioreactor Crypthecodinium cohnii Docosahexaenoic acid Cultivation 



The authors would like to thank the staff of life science engineering and Micro-Electro-Mechanical Systems Laboratory for their assistance.

Author’s contribution

The work was conceived and designed by Dr. H. J. The performance of experiments was done by N.H. The preparation of the manuscript was done by S.A. and N.H. Final revision of the manuscript was done by Dr. H. J. and Prof. A.A.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with animals performed by any of the authors.

Supplementary material

13399_2019_510_MOESM1_ESM.docx (248 kb)
ESM 1 (DOCX 247 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Niloofar Hoseinkhani
    • 1
  • Hasan Jalili
    • 1
    Email author
  • Shahin Ansari
    • 1
  • Abdeltif Amrane
    • 2
  1. 1.Department of Life Science Engineering, Faculty of New Sciences and TechnologiesUniversity of TehranTehranIslamic Republic of Iran
  2. 2.Ecole Nationale Supérieure de Chimie de RennesUniv RennesRennesFrance

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