A comparative investigation of H2O2-involved pretreatments on lignocellulosic biomass for enzymatic hydrolysis

  • Maoyuan Luo
  • Dong Tian
  • Fei ShenEmail author
  • Jinguang Hu
  • Yanzong Zhang
  • Gang Yang
  • Yongmei Zeng
  • Shihuai Deng
  • Yaodong Hu
Original Article


An effective pretreatment to improve cellulose accessibility and facilitate glucose release is crucial in a cellulosic ethanol biorefinery. This work comparatively assessed four H2O2-involved pretreatments, i.e., concentrated H3PO4 plus H2O2 (PHP), H2O2–CH3COOH (HPAC), alkaline-H2O2 (AHP), and Fenton chemistry (FC), for their pretreatment performances on wheat straw, poplar, and birch biomass. Substrate characteristics before and after pretreatment were assessed using SEM, XRD, and LSCM. The hydrolytic potentials of the pretreated substrates were compared by Simons’ stain and cellulose–glucose conversion assessment. The results showed that acidic H2O2-involved pretreatments (PHP and HPAC) were more efficient in biomass delignification compared to AHP and FC. PHP pretreatment is more promising for cellulosic ethanol production due to its corresponding high glucose yield (368.0 mg g−1) after enzymatic hydrolysis.


Lignocellulose Delignification Hemicellulose removal Enzymatic saccharification Enzyme accessibility 



This work was supported by the National Natural Science Foundation of China (grant number 21306120) and the Department of Science and Technology of Sichuan Province (grant numbers 2017HH0047 and 2017SZ0028).

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Ecological and Environmental SciencesSichuan Agricultural UniversityChengduPeople’s Republic of China
  2. 2.Rural Environment Protection Engineering & Technology Center of Sichuan ProvinceSichuan Agricultural UniversityChengduPeople’s Republic of China
  3. 3.Department of Wood ScienceThe University of British ColumbiaVancouverCanada
  4. 4.Department of Bioproducts and Biosystems, School of Chemical EngineeringAalto UniversityAaltoFinland

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