Bioprocessing of cardboard waste for cellulase production
- 102 Downloads
Waste paper, a major source of cellulosic biomass, could be utilized as a potential substrate for cellulase production. In this work, different pretreated waste papers were used as substrates for cellulase production. Among them, cardboard treated with 0.1% H2SO4 was found to be the best substrate for cellulase production by Bacillus subtilis S1 (Accession number MG457704). The optimization of the culture conditions for cellulase production was performed using the Plackett-Burman design (PB) and response surface methodology (RSM). The factors considered for PB design were cardboard concentration, yeast extract, inoculum concentration, cultivation temperature, and pH, with cellulase activity (FPase) as a response. PB design at 30 h was highly significant (F = 0.0018 and R2 = 0.99). Cardboard, yeast extract, and inoculum concentrations were the variables selected for optimization by RSM. The model with 15 runs was highly significant (F = 0.0004). The model that predicted a maximum FPase activity of 2.956 U/mL could be achieved with 7.78% inoculum concentration, 3.99 g/L yeast extract, and 25.89 g/L cardboard. The results showed that the predicted values agreed well with the experimental values. The validation experiment proved the adequacy and accuracy of model. This study demonstrates that cardboard could serve as a low-cost substrate for cellulase production.
KeywordsAcid treatment Bacillus subtilis Cardboard FPase Optimization Response surface method
The authors are greatly thankful to The Research Council (TRC), Oman for the financial support (Grant no: ORG/EBR/14/003).
- 3.Ioelovich M (2014) Waste paper as promising feedstock for production of biofuel. J Sci Res Rep 3:905–916Google Scholar
- 5.Pooja H, Rashmi A, Sabeena K, Bhatkal A, Sastry DN (2016) Production of ethanol from waste newspaper. Int J Eng Trends Technol 5:589–593Google Scholar
- 13.Lee SM, Koo YM (2009) Isolation, purification, characterization of cellulolytic enzymes produced by the isolate Streptomyces omiyaensis. J Microbiol Biotechnol 1:229–233Google Scholar
- 16.Hebeish A, Ibrahim NA (2007) The impact of fronteir sciences on textile industry. Colourage 54:41–55Google Scholar
- 23.Nakamura K, Kappamura K (1982) Isolation and identification of crystalline cellulose hydrolyzing bacterium and its enzymatic properties. J Ferment Technol 60:343–348Google Scholar
- 24.Ariffin H, Abdullah N, Umi K, Shirai Y, Hassan MA (2006) Production and characterization by Bacillus pumilus EB3. Int J Eng Technol 3:47–53Google Scholar
- 30.Ghose TK (1987) Measurment of cellulase activities. Pure Appl Chem 59:2257–2268Google Scholar
- 36.Ioelovich M (2013) Plant biomass as a renewable source of biofuels and biochemicals. Lambert Academic Publishing, GermanyGoogle Scholar
- 37.Patagundi BI, Shivasharan CT, Kaliwal BB (2004) Isolation and characterization of cellulase producing bacteria from soil. Int J Curr Microbiol App Sci 3:59–69Google Scholar
- 38.Dias P, Ramos K, Padilha I, Araujo D, Santos SFM, Silva FLH (2014) Optimization of cellulase production by Bacillus Sp. isolated from sugarcane cultivated soil. Chem Eng Trans 38:277–281Google Scholar
- 43.Rastogi G, Muppidi GL, Gurram RN, Adhikari A, Bischoff KM, Hughes SR, Apel WA, Bang SS, Dixon DJ, Sani RK (2009) Isolation and characterization of cellulose-degrading bacteria from the deep subsurface of the Homestake gold mine, lead, South Dakota, USA. J Ind Microbiol Biotechnol 36:585–598CrossRefGoogle Scholar