Antibacterial Activity of Silver Nanoparticles Isolated from Cow’s Milk, Hen’s Egg White and Lysozyme: A Comparative Study
The aim of the present study is to evaluate the antibacterial activity of biosynthesized silver nanoparticles (AgNPs) with the dietary, nontoxic, eco-friendly biological materials such as raw and pasteurized cow’s milk, egg white and lysozyme. The chosen organisms are clinically important, and hence its in vitro evaluation gains significance in the field of medicine. The AgNPs were characterized by UV–visible spectroscopy which revealed surface plasmon absorbance peaks, ranging between 400 and 450 nm. Fourier transform infrared spectroscopy showed the presence of characteristic C=O and O–H bonds. Transmission electron microscopy revealed spherical particles ranging between 20 and 200 nm. Scanning electron microscopy–energy-dispersive spectroscopy revealed peak at 3 keV confirming the presence of AgNPs. SDS-PAGE analysis further ascertained this with the absence of some protein bands in AgNPs solution as against their respective controls which could indicate its role during the synthesis. The comparative antibacterial activity was determined by well diffusion method. Effective inhibition zones obtained by AgNPs synthesized from the pasteurized milk were 12 mm ± 0.7 against Escherichia coli DH5 \(\upalpha \) and Bacillus subtilis, 14.5 mm ± 0.5 against Pseudomonas alcaligenes and Staphylococcus aureus, 15 mm ± 0.7 against Bacillus cereus. The percentage inhibition displayed by AgNPs from pasteurized cow’s milk was 48%, 58%, 65.9%, 85.7% and 68.2% against the growth of E. coli DH5\(\upalpha \), P. alcaligenes, S. aureus, B. subtilis and B. cereus, respectively, which was significant when compared to the inhibition profiles of AgNPs obtained from other sources such as raw milk, lysozyme and egg white.
KeywordsSilver nanoparticles (AgNPs) Characterization SDS-PAGE Antibacterial Egg white Lysozyme Cow’s milk
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The authors of this study would like to acknowledge V.T.U Research Centre, Muddenahalli, Bangalore, and STIC, Cochin, for providing facility for the characterization works and Aristogene Biosciences Pvt. Ltd. for providing the bacterial strains.
- 9.Ashraf, S.; Chatha, M.A.; Ejaz, W.; Janjua, H.A.; Hussain, I.: Lysozyme-coated silver nanoparticles for differentiating bacterial strains on the basis of antibacterial activity. Res. Lett. 9, 565 (2014)Google Scholar
- 10.Manikprabhu, D.; Lingappa, K.: Antibacterial activity of silver nanoparticles against methicillin-resistant Staphylococcus aureus synthesized using model Streptomyces sp. pigment by photo-irradiation method. J. Pharm. Res. 6, 255–260 (2013)Google Scholar
- 12.Renquan, L.; Yang, D.; Cui, D.; Wang, Z.; Guo, L.: Egg white-mediated green synthesis of silver nanoparticles with excellent biocompatibility and enhanced radiation effects on cancer cells. Int. J. Nanomed. 7, 2101–2107 (2012)Google Scholar
- 13.Bahrami-Teimoori, B.; Nikparast, Y.; Hojatianfar, M.; Akhlaghi, M.: Reza Ghorbani & Hamid Reza Pourianfar, characterisation and antifungal activity of silver nanoparticles biologically synthesised by Amaranthus retroflexus leaf extract. J. Exp. Nanosci. 12(1), 129–139 (2017). https://doi.org/10.1080/17458080.2017.1279355 CrossRefGoogle Scholar
- 14.Khan, Y.; Numan, M.; Ali, M.; Khali, A.T.; Ali, T.; et al.: Bio-synthesized silver nanoparticles using different plant extracts as anti-cancer agent. J. Nanomed. Biother. Discov. (2017). https://doi.org/10.4172/2155-983X.1000154
- 17.Das, B.; Dash, S.K.; Mandal, D.; Ghosh, T.; Chattopadhyay, S.; Tripathy, S.; Das, S.; Dey, S.K.; Das, D.; Roy, S.: Green synthesized silver nanoparticles destroy multidrug resistant bacteria via reactive oxygen species mediated membrane damage. Arab. J. Chem. 10, 862–876 (2015)CrossRefGoogle Scholar
- 18.Hegazi, A.; Hamdy Elshazly, E.; Abdou, A.M.; Abd Allah, F.; Eman, H.; Abdel-Rahman, : Potential antibacterial properties of silver nanoparticles conjugated with cow and camel milks. Glob. Vet. 12(6), 745–749 (2014)Google Scholar
- 22.Naheed, A.; Seema, S.; Singh, V.N.; Shamsi, S.F.; Anjum, F.; Meh, B.R.: Biosynthesis of silver nanoparticles from desmodium triflorum: a novel approach towards weed utilization. Biotechnol. Res. Int. 2011, 8 (2011). https://doi.org/10.4061/2011/454090
- 24.Govarthanan, M.; Selvankumar, T.; Manoharan, K.; Rathika, R.; Shanthi, K.; Lee, K.-J.; Cho, M.; Kamala-Kannan, S.; Oh, B.-T.: Biosynthesis and characterization of silver nanoparticles using panchakavya, an Indian traditional farming formulating agent. Int. J. Nanomed. 9, 1593–1599 (2014)CrossRefGoogle Scholar
- 26.Supriyo, C.; Arpita, B.; Surekha, K.: Green synthesis of protein capped silver nanoparticles from phytopathogenic fungus Macrophomina phaseolina (Tassi) Goid with antimicrobial properties against multidrug-resistant bacteria. Nanoscale Res. Lett. 9(365), 1–11 (2014)Google Scholar
- 27.Kethirabalan, C.; Gurusamy, A.: Bioengineered silver nanobowls using Trichoderma viride and its antibacterial activity against gram-positive and gram-negative bacteria. J. Nanostruct. Chem. 3(9), 1–7 (2013)Google Scholar
- 28.Jie, Z.; Ying, Z.; Bin-song, W.; Maria, S.: Biosynthesis silver nanoparticles using Bacillus Amyloliquefaciens Zxw01 and research on synthesis mechanism. Mater. Sci. Forum 852, 437–442 (2015)Google Scholar