Arabian Journal for Science and Engineering

, Volume 43, Issue 7, pp 3453–3463 | Cite as

The Use of Palm Oil-Based Waste Cooking Oil to Enhance the Production of Polyhydroxybutyrate [P(3HB)] by Cupriavidus necator H16 Strain

  • Hanisah KamilahEmail author
  • Adel Al-Gheethi
  • Tajul Aris Yang
  • Kumar Sudesh
Research Article - Biological Sciences


Waste cooking oil (WCO) is of increasing interest as an inexpensive feedstock to produce biodegradable plastic, poly(3-hydroxybutyrate) [P(3HB)]. In the present study, palm oil-based WCO (PO-WCO) was obtained from nine different locations. Palm oil-based fresh cooking oil (PO-FCO) and PO-WCO were characterised via proximate and physicochemical analysis, prior to being used as carbon sources for the biosynthesis of P(3HB) using Cupriavidus necator H16. It was shown that the free fatty acid, peroxide value, and saturated compounds in all batches of PO-WCO were higher compared to those in the PO-FCO. The cells produced 60–80 wt% P(3HB) with dry cell weight of 14–17 g/L. The weight average molecular weight \((M_{\mathrm{w}})\) was found to be \(1.8 \times 10^{6}\) Da with a polydispersity \((M_{\mathrm{w}}/M_{\mathrm{n}})\) of 2.7 when PO-WCO was used as the carbon source. The PO-WCO was found to be suitable to be used as a sustainable carbon source for cell growth and P(3HB) biosynthesis.


Biomaterial Waste Cooking oil P(3HB) C. necator H16 


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The authors thank the community of Teluk Bahang, Penang, Malaysia, for contributing the PO-WCO used in this study. This study was funded in parts by the Division of Industry and Community Network and Short-Term Research Grants (304/Pbiologi/6311070) from Universiti Sains Malaysia (USM). H. Kamilah acknowledges USM Fellowship during the period of the study.


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Copyright information

© King Fahd University of Petroleum & Minerals 2018

Authors and Affiliations

  1. 1.Food Technology Division, School of Industrial TechnologyUniversiti Sains MalaysiaGelugorMalaysia
  2. 2.Department of Water and Environmental Engineering, Faculty of Civil and Environmental EngineeringUniversiti Tun Hussein Onn Malaysia (UTHM)Batu PahatMalaysia
  3. 3.Ecobiomaterial Research Laboratory, School of Biological SciencesUniversiti Sains MalaysiaGelugorMalaysia

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