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Cognitive impairment and CSF proteome modification after oral bacteriotherapy in HIV patients

  • Claudia Landi
  • Letizia Santinelli
  • Laura Bianchi
  • Enxhi Shaba
  • Giancarlo CeccarelliEmail author
  • Eugenio Nelson Cavallari
  • Cristian Borrazzo
  • Claudia Pinacchio
  • Carolina Scagnolari
  • Vincenzo Vullo
  • Luca Bini
  • Gabriella d’Ettorre
Article
  • 57 Downloads

Abstract

Objective: To investigate whether a probiotic supplementation to cART patients modifies the cerebrospinal fluid (CSF) proteome and improves neurocognitive impairment. Methods: 26 CSF samples from 13 HIV-positive patients [six patients living with HIV (PLHIV) and seven patients with a history of AIDS (PHAIDS)] were analyzed. All patients underwent to neurocognitive evaluation and blood sampling at baseline and after 6 months of oral bacteriotherapy. Immune phenotyping and activation markers (CD38 and HLA-DR) were evaluated on peripheral blood mononuclear cells (PBMC). Plasma levels of IL-6, sCD14, and MIP-1β were detected, by enzyme-linked immunosorbent assay (ELISA). Functional proteomic analysis of CSF sample was conducted by two-dimensional electrophoresis; a multivariate analysis was performed by principal component analysis (PCA) and data were enriched by STRING software. Results: Oral bacteriotherapy leads to an improvement on several cognitive test and neurocognitive performance in both groups of HIV-positive subjects. A reduction in the percentage of CD4+CD38+HLA–DR+ T cells was also observed at peripheral level after the probiotic intake (p = 0.008). In addition, the probiotic supplementation to cART significantly modifies protein species composition and abundance at the CSF level, especially those related to inflammation (β2-microglobulin p = 0.03; haptoglobin p = 0.06; albumin p = 0.003; hemoglobin p = 0.003; immunoglobulin heavy chains constant region p = 0.02, transthyretin p = 0.02) in PLHIV and PHAIDS. Conclusions: Our results suggest that oral bacteriotherapy as a supplement to cART could exert a role in the amelioration of inflammation state at peripheral and CNS level.

Keywords

HIV Probiotic Functional proteomics Immunoactivation Bacteriotherapy 

Notes

Acknowledgments

This study was partially supported by Mendes s.r.l. to Bini Luca.

Data availability statement

In accord to Italian law and regulations on patients’ privacy, data are not publicly available but can eventually be made available upon request.

Author’s contribution

C.L.: Design and conceptualized the study, analyzed the data, and drafted the manuscript for intellectual content. L.S., L.B., and S.E.: Acquired, analyzed, and interpreted the data. G.C.: Enrolled patients, analyzed and interpreted the data, and drafted and revised the manuscript for intellectual content. ENC Enrolled patients, critically revised data and manuscript. CB: critically revised data and performed and revised statistical analysis. C.P.: Acquired, analyzed, and interpreted the data. C.S.: Acquired, analyzed, and interpreted the data, drafted the manuscript for intellectual content. V.V., L.B, and G.d’E.: design and conceptualized study, analyzed the data, drafted the manuscript for intellectual content.

Compliance with ethical standards

The study was approved by the internal committee of the Infectious Diseases Department and by the Ethics Committee of “Sapienza” University of Rome. A written informed consent was obtained from all patients prior to enrollment.

Conflict of interest

Dr. Landi, Dr. Santinelli, Dr. Bianchi, Dr. Shaba, Dr. Ceccarelli, Dr. Pinacchio, Prof. Scagnolari, Prof. Vullo, and Prof. d’Ettorre report no disclosures. Prof. Bini was partially supported by Mendes s.r.l. (Ardea, Roma).

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Copyright information

© Journal of NeuroVirology, Inc. 2019

Authors and Affiliations

  • Claudia Landi
    • 1
  • Letizia Santinelli
    • 2
  • Laura Bianchi
    • 1
  • Enxhi Shaba
    • 1
  • Giancarlo Ceccarelli
    • 3
    Email author
  • Eugenio Nelson Cavallari
    • 3
  • Cristian Borrazzo
    • 3
  • Claudia Pinacchio
    • 3
  • Carolina Scagnolari
    • 2
  • Vincenzo Vullo
    • 3
  • Luca Bini
    • 1
  • Gabriella d’Ettorre
    • 3
  1. 1.Laboratory Functional Proteomics, Department Life SciencesUniversity of SienaSienaItaly
  2. 2.Department of Molecular Medicine, Laboratory of VirologySapienza University of RomeRomeItaly
  3. 3.Department of Public Health and Infectious DiseasesSapienza University of RomeRomeItaly

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