Design and Evaluation of a Gas Chromatograph-Atmospheric Pressure Chemical Ionization Interface for an Exactive Orbitrap Mass Spectrometer
Various separation and mass spectrometric (MS) techniques have furthered our ability to study complex mixtures, and the desire to measure every analyte in a system is of continual interest. For many complex mixtures, such as the total molecular content of a cell, it is becoming apparent that no one single separation technique or analysis is likely to achieve this goal. Therefore, having a variety of tools to measure the complexity of these mixtures is prudent. Orbitrap MSs are broadly used in systems biology studies due to their unique performance characteristics. However, GC-Orbitraps have only recently become available, and instruments that can use gas chromatography (GC) cannot use liquid chromatography (LC) and vice versa. This limits small molecule analyses, such as those that would be employed for metabolomics, lipidomics, or toxicological studies. Thus, a simple, temporary interface was designed for a GC and Thermo Scientific™ Ion Max housing unit. This interface enables either GC or LC separation to be used on the same MS, an Exactive™ Plus Orbitrap, and utilizes an atmospheric pressure chemical ionization (APCI) source. The GC-APCI interface was tested against a commercially available atmospheric pressure photoionization (APPI) interface for three types of analytes that span the breadth of typical GC analyses: fatty acid methyl esters (FAMEs), polyaromatic hydrocarbons (PAHs), and saturated hydrocarbons. The GC-APCI-Orbitrap had similar or improved performance to the APPI and other reported methods in that it had a lower limit of quantitation, better signal to noise, and lower tendency to fragment analytes.
KeywordsGas chromatography Atmospheric pressure chemical ionization (APCI) Atmospheric pressure chemical ionization (APPI) GC-MS Fatty acid methyl esters (FAMEs) Polyaromatic hydrocarbons (PAHs) Saturated hydrocarbons Interface Orbitrap
The authors thank Mr. Tim Free and Mr. Danny Hackworth for the assistance in machining parts and Mr. Matthew Nalepa for the help with the electrical components of the interface. We also acknowledge Dr. Hector F. Castro (Biological and Small Molecule Mass Spectrometry Core, UTK) and Dr. Brandon J. Kennedy for the helpful discussions and instrumental assistance as well as the reviewers for their insightful commentary into the first draft of the manuscript. Mr. Joshua B. Powers was supported by NSF award MCB-1615373. Instrumentation was provided by both NSF award DBI-1530975 and the University of Tennessee Institute of Agriculture.
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