Exploring the Application of the DSA-TOF, a Direct, High-resolution Time-of-Flight Mass Spectrometry Technique for the Screening of Potential Adulterated and Contaminated Herbal Medicines
Global consumption of complementary and alternative medicines, including herbal medicines, has increased substantially, and recent reports of adulteration demonstrate the need for high throughput and extensive pharmacovigilance to ensure product safety and quality. Three different standard reference materials and five previously analyzed herbal medicines have been used as a proof of concept for the application of adulteration/contamination screening using a Direct Sample Analysis (DSA) ion source with TOF MS on the Perkin Elmer AxION 2 TOF. This technique offers the advantages of minimum sample preparation, rapid analysis, and mass accuracies of 5 ppm. The DSA TOF analysis correlates well with the previous analysis on the initial sample set (which found undeclared herbal ingredients), with the added advantage of detecting previously untargeted compounds, including species-specific flavonoids and alkaloids. The rapid analysis using the DSA-TOF facilitates screening for hundreds of compounds in minutes with minimal sample preparation, generating a comprehensive profile for each sample.
KeywordsHerbal medicines Pharmacovigilance Adulteration Contamination Direct mass spectrometry
The authors work is supported by a National Health and Medical Research Council (NHMRC) grant 1061329, and an Australian Government Research Training Program Scholarship awarded to Elly Crighton.
Thanks to Perkin Elmer for their assistance in method development, instrumental access, and training.
Thanks to Rachael Farrington, Christine Nash, Forensic Science SA, and Megan Coghlan for generating the original pharmaceutical adulteration and DNA data in the previous study (manuscript in review).
E.C., G.M., J.W., and R.T. contributed to the design of experiments in this study. E.C. and J.W. conducted the experiments. E.C. analyzed the experimental data. E.C., G.M., R.T., J.W., I.F.M., and M.B. contributed to the preparation and writing of the manuscript. M.B., I.F.M., E.C., G.M., and R.T. contributed to the previous study (manuscript in review).
Compliance with Ethical Standards
The authors declare that they have no competing interests.
- 6.Coghlan, M.L., Maker, G., Crighton, E., Haile, J., Murray, D.C., White, N.E., Byard, R.W., Bellgard, M.I., Mullaney, I., Trengove, R., Allcock, R.J., Nash, C., Hoban, C., Jarrett, K., Edwards, R., Musgrave, I.F., Bunce, M.: Combined DNA, toxicological and heavy metal analyses provides an auditing toolkit to improve pharmacovigilance of traditional Chinese medicine (TCM). Sci. Rep. 5, 17475 (2015)CrossRefGoogle Scholar
- 8.Campbell, N., Clark, J.P., Stecher, V.J., Thomas, J.W., Callanan, A.C., Donnelly, B.F., Goldstein, I., Kaminetsky, J.C.: Adulteration of purported herbal and natural sexual performance enhancement dietary supplements with synthetic phosphodiesterase type 5 inhibitors. J. Sex. Med. 10(7), 1842–1849 (2013)CrossRefGoogle Scholar
- 15.Bhagwat, S., Haytowitz, D.: USDA Database for the Flavonoid Content of Selected Foods, Release 3.2. Nutrient Data Laboratory Home Page: http://www.ars.usda.gov/nutrientdata/flav. Accessed Nov 2017
- 18.TGA, A. G. D. o. H. a. A. T. G. A. Current Year Alerts. https://www.tga.gov.au/current-year-alerts (accessed 14/6/18)