Journal of The American Society for Mass Spectrometry

, Volume 29, Issue 9, pp 1936–1939 | Cite as

Reference Parameters for Protein Hydrogen Exchange Rates

  • David NguyenEmail author
  • Leland Mayne
  • Michael C. Phillips
  • S. Walter Englander
Application Note


The analysis of many hydrogen exchange (HX) experiments depends on knowledge of exchange rates expected for the unstructured protein under the same conditions. We present here some minor adjustments to previously calibrated values and a stringent test of their accuracy.

Graphical Abstract


Hydrogen exchange HDX HX MS 


Funding Information

This work was supported by research grants from the NIH (GM031846), NSF (MCB1020649), and the G. Harold and Leila Y. Mathers Charitable Foundation.

Supplementary material

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Copyright information

© American Society for Mass Spectrometry 2018

Authors and Affiliations

  • David Nguyen
    • 1
    Email author
  • Leland Mayne
    • 1
  • Michael C. Phillips
    • 2
  • S. Walter Englander
    • 1
  1. 1.Johnson Research Foundation, Department of Biochemistry and Biophysics, Perelman School of MedicineUniversity of PennsylvaniaPhiladelphiaUSA
  2. 2.Children’s Hospital of PhiladelphiaPhiladelphiaUSA

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