Journal of The American Society for Mass Spectrometry

, Volume 29, Issue 9, pp 1760–1767 | Cite as

Directed-Backbone Dissociation Following Bond-Specific Carbon-Sulfur UVPD at 213 nm

  • Lance E. Talbert
  • Ryan R. JulianEmail author
Focus: Application of Photons and Radicals for MS: Research Article


Ultraviolet photodissociation or UVPD is an increasingly popular option for tandem-mass spectrometry experiments. UVPD can be carried out at many wavelengths, and it is important to understand how the results will be impacted by this choice. Here, we explore the utility of 213 nm photons for initiating bond-selective fragmentation. It is found that bonds previously determined to be labile at 266 nm, including carbon-iodine and sulfur-sulfur bonds, can also be cleaved with high selectivity at 213 nm. In addition, many carbon-sulfur bonds that are not subject to direct dissociation at 266 nm can be selectively fragmented at 213 nm. This capability can be used to site-specifically create alaninyl radicals that direct backbone dissociation at the radical site, creating diagnostic d-ions. Furthermore, the additional carbon-sulfur bond fragmentation capability leads to signature triplets for fragmentation of disulfide bonds. Absorption of amide bonds can enhance dissociation of nearby labile carbon-sulfur bonds and can be used for stochastic backbone fragmentation typical of UVPD experiments at shorter wavelengths. Several potential applications of the bond-selective fragmentation chemistry observed at 213 nm are discussed.

Graphical Abstract


Laser Photodissociation Excited state Iodine Disulfide Phosphorylation 



The authors gratefully acknowledge assistance from John Syka, Chris Mullen, Chad Weisbrod, Jens Griep-Raming, and Jenny Brodbelt with interfacing the laser with the orbitrap.

Funding Information

The NIH is thanked for financial support (NIGMS grant R01GM107099).

Supplementary material

13361_2018_1934_MOESM1_ESM.docx (614 kb)
ESM 1 (DOCX 614 kb)


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Copyright information

© American Society for Mass Spectrometry 2018

Authors and Affiliations

  1. 1.Department of ChemistryUniversity of California, RiversideRiversideUSA

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