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Novel molecular identification methods for the larger black flour beetle, Cynaeus angustus (Coleoptera: Tenebrionidae)

  • Satoshi FuruiEmail author
  • Akihiro Miyanoshita
  • Taro Imamura
  • Yukio Magariyama
Technical Note
  • 46 Downloads

Abstract

Two new methods were developed for identifying Cynaeus angustus (LeConte) (Coleoptera: Tenebrionidae) by DNA amplification using simplex and real-time PCR targeting the cytochrome c oxidase subunit I (COI) sequence reported previously. The specificities of the PCR primers and probe were also confirmed by the two PCR methods using the 22 main stored-product insect species, including DNA samples from nine tenebrionid beetle species. The results showed that the newly developed simplex and real-time PCR-based methods have sufficient specificity for analysis. The limits of detection for C. angustus total DNA by the simplex and multiplex PCR were 320 fg and 20 pg, respectively.

Keywords

Real-time PCR Qualitative detection Cynaeus angustus (LeConte) Invasive insects 

Notes

Acknowledgements

We thank Dr. Kiyoshi Ando for the morphological identification of C. angustus and Ms. Miyuki Tsuru and Ms. Tomomi Endo for their diligent support to this study.

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Copyright information

© The Japanese Society of Applied Entomology and Zoology 2019

Authors and Affiliations

  • Satoshi Furui
    • 1
    Email author
  • Akihiro Miyanoshita
    • 1
  • Taro Imamura
    • 1
  • Yukio Magariyama
    • 1
  1. 1.Food Research Institute, NAROTsukubaJapan

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