Applied Entomology and Zoology

, Volume 54, Issue 1, pp 123–128 | Cite as

Continuous variation in hind wing length of Galerucella grisescens (Coleoptera, Chrysomelidae, Galerucinae) and genetic basis of wing length determination

  • Shota Nasu
  • Shôichi Imasaka
  • Kunio Suzuki
  • Jun Ito
  • Hideto Hoshina
  • Hiroo Kanno
  • Chika Suyama
  • Makoto TokudaEmail author
Original Research Paper


Dispersal ability is an important trait for insects that affects their survival and distribution ranges. In this study, we focused on variations in hind wing length in Galerucella grisescens (Joannis 1866) (Coleoptera, Chrysomelidae, Galerucinae). We surveyed frequencies of long-winged (LW) and short-winged or micropterous (SW) individuals in various localities in Japan and conducted crossing experiments using LW and SW individuals to clarify the genetic basis of wing length determination. The SW individuals were mainly found in central Honshu (Kanto, Chubu, and Hokuriku districts), while the LW individuals were widely distributed from Hokkaido to the Southwest Islands of Japan. Hybrid offspring derived from LW and SW parents exhibited hind wings of medium length, and the wing length was maternally affected. These results suggest that the hind wing morph of G. grisescens is determined by polygenic quantitative traits, and the gene(s) of interest may occur on the X-chromosome or other maternal factors.


Dispersal ability Leaf beetle Long-winged morph Short-winged morph 



We are indebted to M. Abe, H. Miyauchi, K. Ogata, H. Suenaga and A. Yamawo for their kind offer of materials and to S. Shirahama and U. Okamoto for their kind support in laboratory experiments. This study was supported in part by JSPS KAKENHI Grant Number 26870435 to MT.


  1. Braendle C, Davis GK, Brisson JA, Stern DL (2006) Wing dimorphism in aphids. Heredity 97:192–199CrossRefGoogle Scholar
  2. Danks HV (1987) Insect dormancy: an ecological perspective. Biological Survey of Canada, OttawaGoogle Scholar
  3. Denno RF, Roderick GK, Peterson MA, Huberty AF, Döbel HG, Eubanks MD, Losey JE, Langellotto GA (1996) Habitat persistence underlies intraspecific variation in the dispersal strategies of planthoppers. Ecol Monogr 66:389–408CrossRefGoogle Scholar
  4. Dingle H (1985) Migration. In: Kerkut GA, Gilbert LI (eds) Comprehensive insect physiology, biochemistry and pharmacology. Pergamon Press, Oxford, pp 375–415Google Scholar
  5. Guerra PA (2011) Evaluating the life-history trade-off between dispersal capability and reproduction in wing dimorphic insects: a meta-analysis. Biol Rev 86:813–835CrossRefGoogle Scholar
  6. Harrison RG (1980) Dispersal polymorphism in insects. Annu Rev Ecol Syst 11:95–118CrossRefGoogle Scholar
  7. Johnson CG (1969) Migration and dispersal of insects by flight. Methuen, LondonGoogle Scholar
  8. Kimoto K, Takizawa H (1994) Leaf beetles (Chrysomelidae) of Japan. Tokai University Press, TokyoGoogle Scholar
  9. Matsukura K (2018) Factors leading insect pest outbreaks and preventive pest management: a review of recent outbreaks of forage crop pests in Japan. Jpn J Appl Entomol Zool 62:171–187 (In Japanese.) CrossRefGoogle Scholar
  10. Matsumura M, Suzuki Y (2003) Direct and feeding-induced interactions between two rice planthoppers, Sogatella furcifera and Nilaparvata lugens: effects on dispersal capability and performance. Ecol Entomol 28:174–182CrossRefGoogle Scholar
  11. Mitra C, Wagner WE, Zera JA, Tolle A (2011) Variation in singing behavior among morphs of the sand field cricket, Gryllus firmus. Ecol Entomol 36:152–160CrossRefGoogle Scholar
  12. Nishide Y, Tanaka S (2013) Wing dimorphism in the migratory locust, Locusta migratoria: differentiation of wing morph and phase polyphenism. Entomol Sci 16:421–431Google Scholar
  13. Ohta I, Matsuda K, Matsumoto Y (1998) Feeding stimulation of strawberry leaf beetle, Galerucella vittaticollis Baly (Coleoptera: Chrysomelidae) by quercetin glycosides in polygonaceous plants. Jpn J Appl Entomol Zool 2:45–49 (In Japanese with English summary) CrossRefGoogle Scholar
  14. Pener MP (1985) Hormonal effects on flight and migration. In: Kerkut GA, Gilbert LI (eds) Comprehensive insect physiology biochemistry and phamacology, vol 8. Pergamon Press, New York, pp 491–550Google Scholar
  15. Petitpierre E, Segarra C, Yadav JS, Virkki N (1988) Chromosome numbers and meioformulae of Chrysomelidae. In: Jolivet P, Petitpierre E, Hsiao TH (eds) Biology of Chrysomelidae. Kluwer Academic Publishers, Dordrecht, pp 161–186CrossRefGoogle Scholar
  16. Roff DA (1986) The evolution of wing dimorphism in insects. Evolution 40:1009–1020CrossRefGoogle Scholar
  17. Roff DA (1990) Antagonistic pleiotropy and the evolution of wing dimorphism in Gryllus firmus. Heredity 65:169–177CrossRefGoogle Scholar
  18. Roff DA, Fairbairn DJ (1991) Wing dimorphisms and the evolution of migratory polymorphisms among the insects. Am Zool 31:243–251CrossRefGoogle Scholar
  19. Suzuki N (1985) Resource utilization of three chrysomelid beetles feeding on Rumex plants with diverse vegetational background. Jpn J Ecol 35:225–234Google Scholar
  20. Suzuki N (1986) Interspecific competition and coexistence of the two chrysomelids, Gastrophysa atrocyanea Motschulsky and Galerucella vittaticollis Baly (Coleoptera: Chrysomelidae), under limited food resource conditions. Ecol Res 1:259–268CrossRefGoogle Scholar
  21. Suzuki N (1987) Chrysomelid communities on Rumex Japonicus. In: Kimoto S, Takeda H (eds) Insect Communities in Japan. Tokai University Press, Tokyo, pp 141–148 (In Japanese) Google Scholar
  22. Suzuki K (1994) Comparative morphology of the hindwing venation of the Chrysomelidae (Coleoptera). In: Jolivet PH, Cox ML, Petitpierre E (eds) Novel aspects of the biology of Chrysomelidae. Kluwer Academic Publishers, Dordrecht, pp 337–354CrossRefGoogle Scholar
  23. Tanaka S (2006) Corazonin and locust phase polyphenism. Appl Entomol Zool 41:179–193CrossRefGoogle Scholar
  24. Tanaka S, Katagiri C, Arai T, Nakamura K (2001) Continuous variation in wing length and flight musculature in a tropical field cricket, Teleogryllus derelictus: implications for the evolution of wing dimorphism. Entomol Sci 4:195–208Google Scholar
  25. Tauber MJ, Tauber CA, Masaki S (1986) Seasonal adaptations of insects. Oxford University Press, New YorkGoogle Scholar
  26. R Core Team (2017) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Accessed 8 July 2018
  27. Tokuda M (2015) Host specificity and wing polymorphism in Galerucella grisescens (Coleoptera: Chrysomelidae). Nat Insects 50(12):8–12 (In Japanese) Google Scholar
  28. Zeng Y, Zhu DH (2012) Trade-off between flight capability and reproduction in male Velarifictorus asperses crickets. Ecol Entomol 37:244–251CrossRefGoogle Scholar
  29. Zera AJ (2003) The endocrine regulation of wing polymorphism in insects: state of the art, recent surprises, and future directions. Integr Comparat Biol 43:607–616CrossRefGoogle Scholar
  30. Zera AJ, Brisson JA (2012) Quantitative, physiological, and molecular genetics of dispersal and migration. In: Colbert J, Baguette M, Benton T, Bullock J (eds) Dispersal ecology and evolution. Oxford University Press, Oxford, pp 63–82CrossRefGoogle Scholar
  31. Zera AJ, Denno RF (1997) Physiology and ecology of dispersal polymorphism in insects. Annu Rev Entomol 42:207–231CrossRefGoogle Scholar
  32. Zhao LQ, Zhu DH, Zeng Y (2010) Physiological trade-offs between flight muscle and reproductive development in the wing dimorphic cricket Velarifictorus ornatus. Entomol Exp Appl 135:288–294CrossRefGoogle Scholar
  33. Zheng C, Ovaskainen O, Hanski I (2009) Modelling single nucleotide effects in phosphoglucose isomerase on dispersal in the Glanville fritillary butterfly: coupling of ecological and evolutionary dynamics. Phil Trans R Soc B 364:1519–1532CrossRefGoogle Scholar

Copyright information

© The Japanese Society of Applied Entomology and Zoology 2019

Authors and Affiliations

  1. 1.Laboratory of Systems Ecology, Faculty of AgricultureSaga UniversitySagaJapan
  2. 2.E-AssistKurumeJapan
  3. 3.Minami-TaikôyamaImizuJapan
  4. 4.Oshitatechô, FuchuTokyoJapan
  5. 5.Faculty of EducationFukui UniversityFukuiJapan
  6. 6.Higashi-HonchoJoetsuJapan
  7. 7.Faculty of EducationGifu UniversityGifuJapan
  8. 8.The United Graduate School of Agricultural SciencesKagoshima UniversityKagoshimaJapan

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