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Development of polymorphic microsatellite loci for Ceratosolen gravelyi (Hymenoptera: Agaonidae), the pollinating wasp of Ficus semicordata (Urticales: Moraceae)

  • Li-Yuan Yang
  • Ai-Ying Wang
  • Zhu-Zeng Huang
  • Rui-Wu Wang
  • Da-Yong Zhang
Technical Note
  • 4 Downloads

Abstract

The interaction between figs and fig-pollinating wasps constitute one of the most tightly integrated pollination mutualisms. The use of molecular markers, especially microsatellite markers, has improved our understanding of important aspects of this mutualism like sex-ratio evolution, the presence of cryptic pollinator species, and its phylogeographic history. From an enriched (AG)n/(AC)n library, we developed ten polymorphic microsatellite loci for the fig-pollinating wasp Ceratosolen gravelyi, the pollinator of Ficus semicordata. A total of 74 alleles were detected in the population comprising of 41 individuals from three crops in the Xishuangbanna tropical rainforest. A number of alleles per locus ranged from 3 to 10. The observed heterozygosity was 0.537 ± 0.111 and the expected heterozygosity was 0.706 ± 0.112. No linkage disequilibrium was found between any two of the ten microsatellite loci. The newly isolated microsatellite markers will be very useful for wasp parentage analysis and the studying of fig-pollinating wasp sex-ratio mechanism.

Keywords

Fig-pollinating wasp Heterozygosity Mutualism Microsatellite loci 

Notes

Funding

This study was funded by the National Natural Science Foundation of China (31700328), the China Postdoctoral Science Foundation (2017M613194), and the Open Fund of Key Laboratory of Biodiversity Science and Ecological Engineering, Ministry of Education.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13355_2018_592_MOESM1_ESM.docx (25 kb)
Supplementary material 1 (DOCX 24 kb)

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Copyright information

© The Japanese Society of Applied Entomology and Zoology 2018

Authors and Affiliations

  1. 1.Center for Ecological and Environmental SciencesNorthwestern Polytechnical UniversityXi’anChina
  2. 2.State Key Laboratory of Earth Surface Processes and Resource Ecology and Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, College of Life SciencesBeijing Normal UniversityBeijingChina
  3. 3.Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglunChina

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