Applied Entomology and Zoology

, Volume 54, Issue 1, pp 55–62 | Cite as

Distribution and population structure of two phylogroups of the parasitoid Encarsia smithi (Hymenoptera: Aphelinidae) in tea fields infested with the invasive camellia spiny whitefly Aleurocanthus camelliae (Hemiptera: Aleyrodidae) in Shizuoka Prefecture, Japan

  • Kaori YaraEmail author
  • Ryuji Uesugi
  • Takeshi Shimoda
  • Yasushi Sato
Original Research Paper


A recent study revealed that two phylogenetic groups of the parasitoid Encarsia smithi (Hymenoptera: Aphelinidae) can attack the camellia spiny whitefly Aleurocanthus camelliae (Hemiptera: Aleyrodidae), an invasive pest of Japanese tea fields. Type I was introduced in 1925 from China to Japanese citrus orchards to control the citrus spiny whitefly A. spiniferus, but it has also recently appeared in several tea fields. Type II, presumably introduced accidentally, was also found in many tea fields. However, little is known about distribution and their relative importance as a biocontrol agent in tea fields. To investigate these aspects, we developed specific PCR for the two groups using a variation in their nuclear ribosomal DNA’s ITS region. We then surveyed their distribution in 23 tea fields in Shizuoka Prefecture, Japan, from 2013 to 2015 using this specific PCR. We found that both types were distributed, sometimes coexisting, in many tea fields during 2013–2015, although the population structure of these types varied with the field, year and season. These results suggest that A. camelliae can be controlled unintentionally by accidentally introduced exotic natural enemies (Type II) and/or Type I species originally introduced to control other invasive pests such as A. spiniferus.


Aleurocanthus camelliae Encarsia smithi Parasitoid wasp Phylogenetic group Specific PCR 



We thank the editor-in-chief and the two anonymous reviewers for their extremely helpful comments.


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Copyright information

© The Japanese Society of Applied Entomology and Zoology 2018

Authors and Affiliations

  • Kaori Yara
    • 1
    • 3
    Email author
  • Ryuji Uesugi
    • 1
    • 4
  • Takeshi Shimoda
    • 2
  • Yasushi Sato
    • 1
  1. 1.Kanaya Tea Research Station, Institute of Fruit Tree and Tea Science, NAROShimadaJapan
  2. 2.Central Region Agricultural Research Center, NAROTsukubaJapan
  3. 3.Institute of Fruit Tree and Tea Science, NAROTsukubaJapan
  4. 4.Tohoku Agricultural Research Center, NAROMoriokaJapan

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