MicroRNA transcriptome analysis of poly I:C-stimulated and PRRSV-infected porcine alveolar macrophages
Porcine reproductive and respiratory syndrome virus (PRRSV) causes severe reproductive failure in sows, respiratory diseases, and high mortality in piglets, which results in serious economic losses to the swine industry worldwide. Previous studies have described that PRRSV could suppress the host immune system and had antiapoptotic activity in its initial phase of infection. Polyinosinic-polycytidylic acid (poly I:C), a synthesized analogue of viral double-strand RNA, activates innate immunity responses and induces apoptosis in cells. Therefore, we performed miRNA transcriptome analysis of poly I:C–stimulated and PRRSV-infected porcine alveolar macrophages (PAMs) using deep sequencing technology, to compare the different miRNA profiles between the statuses of innate immune activation and inactivation. After sequencing, 267 known mature miRNAs and 64 novel miRNAs were observed in PAMs, and a total of 197 miRNAs were significantly differently expressed in poly I:C–stimulated PAMs, compared with mock control cells. Thirty-three of them were also significantly alerted in PRRSV-infected PAMs. This indicated that PRRSV only slightly alerted the miRNA expression profile of host cells compared with poly I:C–stimulated PAMs, which confirmed that PRRSV could suppress host innate immune responses during the early stages of infection. Among the differentially expressed miRNAs, we found that ssc-miR-27b-3p could significantly inhibit PRRSV RNA and protein replication in MARC-145 cells and PAMs. Its antiviral mechanism needs further research in the future.
KeywordsPRRSV Poly I:C Innate immune Apoptosis miR-27b
The authors thank Professor Shaobo Xiao from Huazhong Agricultural University for providing the Nsp2 antibody.
This study received financial assistance from the National Natural Science Foundation (31501924), Hubei Natural Science Foundation (2015CFA105 and 2018CFA014), Hubei Province Technical Innovation Project (2016ABA117 and 2017ABA134), and Hubei Agricultural Science and Technology Innovation Center (2016-620-000-001-022).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
All animal procedures were carried out according to protocols approved by the Biological Studies Animal Care and Use Committee of Hubei Province, China.
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