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Journal of Applied Genetics

, Volume 60, Issue 3–4, pp 375–383 | Cite as

MicroRNA transcriptome analysis of poly I:C-stimulated and PRRSV-infected porcine alveolar macrophages

  • Junjing Wu
  • Ziyun Ji
  • Mu Qiao
  • Xianwen Peng
  • Huayu Wu
  • Zhongxu Song
  • Haizhong Zhao
  • Guisheng Liu
  • Fenge LiEmail author
  • Shuqi MeiEmail author
Animal Genetics • Original Paper

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) causes severe reproductive failure in sows, respiratory diseases, and high mortality in piglets, which results in serious economic losses to the swine industry worldwide. Previous studies have described that PRRSV could suppress the host immune system and had antiapoptotic activity in its initial phase of infection. Polyinosinic-polycytidylic acid (poly I:C), a synthesized analogue of viral double-strand RNA, activates innate immunity responses and induces apoptosis in cells. Therefore, we performed miRNA transcriptome analysis of poly I:C–stimulated and PRRSV-infected porcine alveolar macrophages (PAMs) using deep sequencing technology, to compare the different miRNA profiles between the statuses of innate immune activation and inactivation. After sequencing, 267 known mature miRNAs and 64 novel miRNAs were observed in PAMs, and a total of 197 miRNAs were significantly differently expressed in poly I:C–stimulated PAMs, compared with mock control cells. Thirty-three of them were also significantly alerted in PRRSV-infected PAMs. This indicated that PRRSV only slightly alerted the miRNA expression profile of host cells compared with poly I:C–stimulated PAMs, which confirmed that PRRSV could suppress host innate immune responses during the early stages of infection. Among the differentially expressed miRNAs, we found that ssc-miR-27b-3p could significantly inhibit PRRSV RNA and protein replication in MARC-145 cells and PAMs. Its antiviral mechanism needs further research in the future.

Keywords

PRRSV Poly I:C Innate immune Apoptosis miR-27b 

Notes

Acknowledgments

The authors thank Professor Shaobo Xiao from Huazhong Agricultural University for providing the Nsp2 antibody.

Funding

This study received financial assistance from the National Natural Science Foundation (31501924), Hubei Natural Science Foundation (2015CFA105 and 2018CFA014), Hubei Province Technical Innovation Project (2016ABA117 and 2017ABA134), and Hubei Agricultural Science and Technology Innovation Center (2016-620-000-001-022).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

All animal procedures were carried out according to protocols approved by the Biological Studies Animal Care and Use Committee of Hubei Province, China.

Supplementary material

13353_2019_500_MOESM1_ESM.doc (520 kb)
ESM 1 (DOC 520 kb)
13353_2019_500_MOESM2_ESM.doc (276 kb)
ESM 2 (DOC 276 kb)

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Copyright information

© Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2019

Authors and Affiliations

  1. 1.Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and VeterinaryHubei Provincial Academy of Agricultural SciencesWuhanChina
  2. 2.Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of EducationHuazhong Agricultural UniversityWuhanChina

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