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Drug Delivery and Translational Research

, Volume 9, Issue 6, pp 1057–1066 | Cite as

Total drug quantification in prodrugs using an automated elemental analyzer

  • Yingwen Hu
  • David M. Stevens
  • Sonny Man
  • Rachael M. Crist
  • Jeffrey D. ClogstonEmail author
Original Article
First Online:
  • 63 Downloads

Abstract

Polymeric prodrugs have become an increasingly popular strategy for improving the pharmacokinetic properties of active pharmaceutical ingredients (API). Therefore, identifying a robust method for quantification of the API in these prodrug products is a key part of the drug development process. Current drug quantification methods include hydrolysis followed by reversed phase high-performance liquid chromatography (RP-HPLC), size exclusion chromatography (SEC)-based molecular weight determination, and mass spectrometry. These methods tend to be time-consuming and often require challenging method development. Here, we present a comparative study highlighting the automated elemental analyzer as a facile approach to drug quantification in this up-and-coming class of therapeutics. A polymeric prodrug using poly(l-lysine succinylated) (PLS) and the drug lamivudine (LAM) was prepared and analyzed using the elemental analyzer in comparison to the traditional approaches of hydrolysis followed by RP-HPLC and SEC using multi-angle light scattering (MALS) detection. The elemental analysis approach showed excellent agreement with the conventional methods but proved much less laborious, highlighting this as a rapid and sensitive analytical method for the quantitative determination of drug loading in polymeric prodrug products.

Keywords

Elemental analysis Drug loading Polymer–drug conjugates Nanoparticle Nanomedicine Prodrug 
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Notes

Funding information

This project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. HHSN261200800001E.

Compliance with ethical standards

Disclaimer

The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

13346_2019_649_MOESM1_ESM.docx (54 kb)
ESM 1 (DOCX 54 kb)

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Copyright information

© Controlled Release Society 2019

Authors and Affiliations

  1. 1.Nanotechnology Characterization Laboratory, Cancer Research Technology Program, Leidos Biomedical Research, Inc.Frederick National Laboratory for Cancer ResearchFrederickUSA

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