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Drug Delivery and Translational Research

, Volume 9, Issue 5, pp 997–1007 | Cite as

Design and evaluation of anti-fibrosis drug engineered resealed erythrocytes for targeted delivery

  • Piyali Dey
  • Subham Banerjee
  • Santa Mandal
  • Pronobesh ChattopadhyayEmail author
Original Article
  • 110 Downloads

Abstract

Resealed erythrocytes (RSE) are potential, site-specific carrier system for drug delivery with prolonged drug release activity. In this study, erythrocytes obtained from Wistar albino rats were loaded with ambroxol hydrochloride (AH) with the focus to convenience the lung targeting possibility of the carrier erythrocytes. AH loading in erythrocytes using preswell dilution technique with glutaraldehyde (GA) as a cross-linking agent was evaluated and validated. Drug-loaded erythrocyte was characterized in terms of in vitro drug release followed by osmotic fragility study which showed amplified drug entrapment efficiency (DEE) and hemoglobin content values as well. In vivo lung fibrosis study, rats were sensitized to egg albumin by intraperitoneal (i.p.) injection and then inhalation in a whole body inhalation chamber. A sign of inflammation, airway sub-mucosal fibrosis, hypertrophy, and hyperplasia was observed. A series of in vivo studies were carried out to describe the effect of AH-loaded RSE including measurement of cytokines in Bronchoalveolar Lavage (BAL) fluid and histopathology study. AH showed a stepwise reduced level of cytokines in BAL at a different time interval after being injected of AH-loaded RSE. Furthermore, in vivo lung distribution experiments were performed for optimized formulation, and degree of distribution of the drugs inside the targeted organ was found to be satisfactory.

Keywords

Resealed erythrocytes Ambroxol hydrochloride Preswell dilution technique Glutaraldehyde Lung fibrosis 

Notes

Acknowledgments

The authors are thankful to Director, DRL, Tezpur, Assam, India for providing all necessary facility to bearing this research work. Sincere thanks also to the scientists and all staff of Division of Pharmaceutical Technology Division DRL, Tezpur, Assam, for cooperation while carrying out the work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Controlled Release Society 2019

Authors and Affiliations

  • Piyali Dey
    • 1
  • Subham Banerjee
    • 2
  • Santa Mandal
    • 1
  • Pronobesh Chattopadhyay
    • 1
    Email author
  1. 1.Division of Pharmaceutical TechnologyDefence Research LaboratoryTezpurIndia
  2. 2.Department of PharmaceuticsNational Institute of Pharmaceutical Education and Research (NIPER)GuwahatiIndia

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