Evaluation of collagen type I scaffolds including gelatin-collagen microparticles and Aloe vera in a model of full-thickness skin wound
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Research on collagen type I scaffolds with Aloe vera is sparse. The aim of this work was to develop collagen type I scaffolds with gelatin-collagen microparticles and loaded with a dispersion of A. vera, to assess their performance as grafting material for healing of skin wounds. Scaffolds were evaluated in a Cavia porcellus model with full-thickness skin wound and compared with wounds healed by secondary intention (controls). Animals grafted with scaffolds without A. vera and their control wounds were also included in the study. Evaluation of enzymatic degradation and percentage of the scaffolds’ free amino groups—as an indirect assessment of their cross-linking—were also carried out because A. vera contains compounds which affect their stability. We found that dispersions of lyophilized A. vera extract loaded on scaffolds do not have cytotoxic potential, and they decrease collagenase degradation of scaffolds in the range of 0.1 to 0.3% w/v in a dose-dependent manner. Only the A. vera dispersion with the highest concentration (0.3% w/v) decreased the percentage of free amino groups, which are the ones involved in the cross-link of collagen fibers. This finding suggests that cross-linking is not the mechanism by which the tested dispersions stabilize the scaffolds. Preclinical, histochemical, and histomorphometric analyses of repaired wound tissue indicate that loading collagen type I scaffolds, including microparticles of gelatin-collagen, with A. vera in the concentrations tested does not improve wound healing. Low biodegradability of the tested scaffolds caused by the inhibition of collagenase activity might account for these results.
KeywordsAloe vera Collagen type I scaffolds Microparticles of gelatin-collagen Collagenase degradation Cross-linking Wound healing Total-thickness skin wounds
The authors would like to thank Dr. Felipe Cabello for the critical reading of the manuscript and his suggestions for improving it, Ph.D. candidate Julia Morales and Dr. Manuel Morales for editing the English, Ph.D. candidate Diana Millán for helping with the edition of the figures, and DVM Alejandra Muñoz for helping with the animal experiments.
This work was supported by the Colombian Administrative Department of Science, Technology and Innovation (Colciencias) Grant 1101-569-34982.
Compliance of ethical standards
All institutional and national guidelines for the care and use of laboratory animal were followed.
Conflict of interest
The authors declare that they have no conflict of interest.
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