Isolation and recombinant analysis of variants of porcine epidemic diarrhea virus strains from Beijing, China

  • Peng Wang
  • Jinyan Zhu
  • Xinze Liu
  • Jiaojiao Guo
  • Xuejia Gu
  • Wenke RuanEmail author
Original Article


Porcine epidemic diarrhea virus (PEDV) is a highly infectious virus infecting pigs with high morbidity, especially for newborn piglets. Several PEDV strains were isolated from the intestinal tracts of diarrheic piglets from the Beijing area, China. Sequencing of the whole-genome of the PEDV isolates (GenBank numbers MG546687-MG546690) yielded sequences of 28033–28038 nt. The phylogenetic tree revealed that these strains from the Beijing area belonged to group II, while the vaccine strain, CV777, belonged to group I. We also determined the genetic correlation between these strains and CV777 strain. However, it showed that these strains in the Beijing area had unique mutations. The sequence identity of PEDV strains showed that these strains are most similar to these strains LZW, CH/JX-1/2013, USAIllinois972013, USAKansas1252014, CH/GDZQ/2014, SHQPYM2013, AJ1102, CHZMDZY11, KoreaK14JB01, and CHYJ130330, respectively. The possible recombination events indicate that PEDV in this studies were possibly recombinant strain formed by parent strains USAIllinois972013, KoreaK14JB01, CHYJ130330, and CHZMDZY11. These PEDV strains has been genetic recombination and mutations. The variant strains characterized in this study help to the evolutionary analysis of PEDV.


PEDV Sequence analysis Isolation Phylogenetic tree Whole-genome 



The authors would like to thank the pig farm owners their collaboration.


This work was supported by the National Natural Science Foundation of China (No. 31572499). The funders did not play any role in the design, conclusions or interpretation of the study.

Conflict of interest

The authors declare that they have no competing interests.

Ethics approval and consent to participate

Experimental animals used in the study were cared for in accordance with the internationally accepted standards for the care and handling of laboratory animals. All procedures were approved by the Animal Care and Use Committee of Beijing University of Agriculture (Beijing, China). The Animal Ethics Committee approval number was BUAACUC2016-1. All samples were collected after informed written consent was obtained from the owners.


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Copyright information

© Indian Virological Society 2019

Authors and Affiliations

  1. 1.Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, College of Animal Science and TechnologyBeijing University of AgricultureBeijingChina

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