Temperature sensitivity and environmental stability of Chandipura virus

  • A. B. SudeepEmail author
  • R. S. Gunjikar
  • Y. S. Ghodke
  • K. Khutwad
  • G. N. Sapkal
Original Article


Chandipura virus (CHPV), a negative-stranded RNA virus of family Rhabdoviridae is endemic in Central India since 1965. The virus gained public health importance when it was held responsible for massive outbreak in 2003–2004 in Maharashtra, Telengana and Gujarat with case fatality rates ranging from 55 to 75% among children. We studied the stability of the virus as well as RNA persistence in samples stored at different temperatures for different periods. CHPV remained infective in sand flies and cell culture supernatants at 4 °C for 8 weeks. At 37 °C CHPV remained viable for 18 days when stored in infected cell supernatant (Minimum essential medium supplemented with 10% fetal bovine serum). However, in infected sand flies stored at 37 °C, the virus lost virulence within a week. CHPV RNA, though lost virulence, could be detected in virus exposed sand flies stored at 37 °C for 13 weeks by real time RT-PCR. Retaining virulence at 37 °C for 18 days in serum containing medium is a matter of concern for laboratories and hospital settings where clinical samples are handled. RNA persistence for prolonged periods in dead sand flies might help in surveillance studies of CHPV in sand flies and will help in resource constraint nations where cold chain management is a concern.


Chandipura virus Environmental stability Real time RT-PCR RNA persistence Sand flies 



The authors thank the Director, National Institute of Virology, Pune for his interest and constant support during the study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. 1.
    Bhatt PN, Rodrigues FM. Chandipura: a new arbovirus isolated in India from patients with febrile illness. Indian J Med Res. 1967;55:1295–305.Google Scholar
  2. 2.
    Chadha MS, Arankalle VA, Jadi RS, Joshi MV, Thakare JP, Mahadev PVM, Mishra AC. An outbreak of Chandipura virus encephalitis in the eastern districts of Gujarat state, India. Am J Trop Med Hyg. 2005;73:566–70.CrossRefGoogle Scholar
  3. 3.
    Dwibedi B, Sabat J, Hazra RK, Kumar A, Dinesh DS, Kar SK. Chandipura virus infection causing encephalitis in a tribal population of Odisha in eastern India. Natl Med J India. 2015;28:185–7.Google Scholar
  4. 4.
    Ghosh S, Basu A. Neuropathogenesis by Chandipura virus: an acute encephalitis syndrome in India. Natl Med J India. 2017;30:21–5.Google Scholar
  5. 5.
    Ghosh S, Dutta K, Basu A. Chandipura virus induces neuronal death through Fas-mediated extrinsic pathway. J Virol. 2013;87:12398–406.CrossRefGoogle Scholar
  6. 6.
    Kumar S, Jadi RS, Sudeep AB, Tandale BV, Mishra AC, Arankalle VA. Development and evaluation of a real-time one-step reverse-transcriptase PCR for quantitation of Chandipura virus. BMC Infect Dis. 2008;8:168. Scholar
  7. 7.
    Mavale MS, Fulmali PV, Ghodke YS, Mishra AC, Kanojia P, Geevarghese G. Experimental transmission of Chandipura virus by Phlebotomus argentipes (Diptera: Psychodidae). Am J Trop Med Hyg. 2007;76:307–9.CrossRefGoogle Scholar
  8. 8.
    Menghani S, Chikhale R, Raval A, Wadibhasme P, Khedekar P. Chandipura virus: an emerging tropical pathogen. Acta Trop. 2012;124:1–14.CrossRefGoogle Scholar
  9. 9.
    Mishra AC. Chandipura encephalitis: a newly recognized disease of public health importance in India. In: Scheld WM, Hooper DC, Hughes JM, editors. Emerging infections, chapter 7. Washington, DC: ASM Press; 2007. p. 121–37.Google Scholar
  10. 10.
    Muller JA, Harms M, Schubert A, Jansen S, Michel D, Mertens T, Schmidt-Chanasit J, Munch J. Inactivation and environmental stability of Zika virus. Emerg Infect Dis. 2016;22:1685–7.CrossRefGoogle Scholar
  11. 11.
    Parashar D, Sudeep AB, More A, Walimbe AM, Mavale M, Amdekar S. Persistence of chikungunya virus in samples stored at different temperatures. Curr Sci. 2018;115:25–7.Google Scholar
  12. 12.
    Rao BL, Basu A, Wairagkar NS, Gore MM, Arankalle VA, Thakare JP, Jadi RS, Rao KA, Mishra AC. A large outbreak of acute encephalitis with high fatality rate in children in Andhra Pradesh, India, in 2003, associated with Chandipura virus. Lancet. 2004;364:869–74.CrossRefGoogle Scholar
  13. 13.
    Reed LJ, Muench HA. A simple method for estimating fifty percent endpoint. Am J Hyg. 1938;27:493–7.Google Scholar
  14. 14.
    Sudeep AB, Bondre VP, Gurav YK, Gokhale MD, Sapkal GN, Mavale MS, George RP, Mishra AC. Isolation of Chandipura virus from Sergentomyia species of sand flies collected during an outbreak investigation of encephalitis in Vidarbha region, Maharashtra, India. Indian J Med Res. 2014;139:26–9.Google Scholar
  15. 15.
    Sudeep AB, Paingankar M, Ghodke YS, George RP, Aher RV, Gokhale MD. Vector competence of two Indian populations of Culex quinquefasciatus (Diptera: Culicidae) mosquitoes to three West Nile virus strains. J Vector Borne Dis. 2015;52:185–92.Google Scholar
  16. 16.
    Sudeep AB, Gurav YK, Bondre VP. Changing clinical scenario in Chandipura virus infection. Indian J Med Res. 2016;143:712–21.CrossRefGoogle Scholar
  17. 17.
    Tandale BV, Tikute SS, Arankalle VA, Sathe PS, Joshi MV, Ranadive SN, Kanojia PC, Eshwarachary D, Kumarswamy M, Mishra AC. Chandipura virus: a major cause of acute encephalitis in children in North Telangana, Andhra Pradesh, India. J Med Virol. 2008;80:118–24.CrossRefGoogle Scholar
  18. 18.
    Tesh RB, Modi GB. Growth and transovarial transmission of Chandipura virus (Rhabdoviridae: Vesiculovirus) in Phlebotomus papatasi. Am J Trop Med Hyg. 1983;32:621–3.CrossRefGoogle Scholar
  19. 19.
    Verma AK, Ghosh S, Pradhan S, Basu A. Microglial activation induces neuronal death in Chandipura virus infection. Sci Rep. 2016. Scholar

Copyright information

© Indian Virological Society 2019

Authors and Affiliations

  • A. B. Sudeep
    • 1
    Email author
  • R. S. Gunjikar
    • 1
  • Y. S. Ghodke
    • 1
  • K. Khutwad
    • 1
  • G. N. Sapkal
    • 1
  1. 1.ICMR-National Institute of VirologyPuneIndia

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