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Occurrence and phylogenetic analysis of avipoxvirus isolated from birds around Jammu

  • Bhavesh Sharma
  • Nawab NashiruddullahEmail author
  • Mohd. Altaf Bhat
  • Anil Taku
  • Parimal Roychoudhury
  • Jafrin Ara Ahmed
  • Shilpa Sood
  • Syed Mehmood
Original Article
  • 14 Downloads

Abstract

Domesticated fowls, pigeons and turkey birds were screened for avipoxvirus infection from different areas in Jammu region. Based on typical pox lesions the overall occurrence in fowl was found to be 18.52%, 17.03% in pigeons and 57.14% in turkeys. Mortality recorded in chicks was 41.96%, 45.36% in squabs, 100% in poults, and 20.00% in adult turkeys. Both cutaneous and diphtheritic forms of the disease was observed of which the latter was particularly prevalent in young birds. One sample of putative fowlpox virus (FWPV) from skin lesions of a fowl, and two samples of putative pigeonpox virus (PGPV) from skin and diphtheritic lesions each were inoculated on chorio-allantoic membrane (CAM) of 10–12 days old chicken embryonated eggs. A confirmatory diagnosis was made by PCR amplification of a highly conserved P4b gene locus detected in tissue samples from skin, diphtheritic membrane and virus inoculated CAM yielding a predicted 578 bp product. Phylogenetic analysis based on the same P4b gene locus revealed FWPV and turkeypox virus (TKPV) to be 99% related and belonging to clade 1, while PGPV was found to belong to clade 2. All three isolates illustrate considerable heterogeneity within the conserved P4b gene locus. The study indicates that the closely related FWPV and TKPV isolates may have the potential of cross infection between fowls and turkeys and therefore cross transmission studies are suggested.

Keywords

Avipoxvirus FWPV PGPV TKPV CAM P4b Phylogeny 

Notes

Supplementary material

13337_2018_507_MOESM1_ESM.docx (46 kb)
Supplementary material 1 (DOCX 46 kb)

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Copyright information

© Indian Virological Society 2019

Authors and Affiliations

  1. 1.Division of Veterinary Pathology, Faculty of Veterinary Sciences and Animal HusbandrySher-e-Kashmir University of Agricultural Sciences and Technology-JammuRS PuraIndia
  2. 2.Division of Veterinary Microbiology, Faculty of Veterinary Sciences and Animal HusbandrySher-e-Kashmir University of Agricultural Sciences and Technology-JammuRS PuraIndia
  3. 3.Department of Veterinary Microbiology, College of Veterinary Sciences and Animal HusbandryCentral Agricultural University, SelesihAizawlIndia
  4. 4.Division of Veterinary Physiology and Biochemistry, Faculty of Veterinary Sciences and Animal HusbandrySher-e-Kashmir University of Agricultural Sciences and Technology-JammuRS PuraIndia

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