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Development of a PCR test for detection of Xanthomonas campestris pv. raphani

  • Mehede Hassan Rubel
  • Mohammad Rashed Hossain
  • Ujjal Kumar Nath
  • Sathishkumar Natarajan
  • Ji-Hee Lee
  • Hee-Jeong Jung
  • Hoy-Taek Kim
  • Jong-In Park
  • Ill-Sup NouEmail author
Research Note
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Abstract

Leaf spot of Brassica is an important bacterial seed borne disease caused by X. campestris pv. raphani (Xcr), one of the three pathovars of Xanthomonas campestris species which makes significant economic loss throughout the world. A rapid and reliable detection method will be helpful for early diagnosis of the disease. To do that, we designed Xcr specific sequence-characterized amplified region (SCAR) markers from the Xcr specific genomic fragments, identified by aligning the whole genome sequences of several closely related bacterial pathovars and other bacterial species. Our primer set Xcr_59 produced Xcr specific amplicon of 679 bp in PCR assays. This primer pair was also capable of detecting Xcr strains directly from the artificially infected leaf samples prepared by suspending the diseased leaf area in sterile water without extracting bacterial DNA. This rapid, sensitive and reliable detection technique will be useful for adopting any firsthand plant protection measures against the disease.

Keywords

X. campestris pv. raphani campestris pv Raphani Leaf spot PCR marker Brassica 

Notes

Acknowledgments

We thank Joana G. Vicente, University of Warwick, UK for providing different strains with known races of Xcc, Xci and Xcr isolates and the Korean Agriculture Culture Collection (KACC), Korea and the International Collection of Microorganisms from Plants (ICMP), New Zealand for providing respective bacterial strains. This work was supported by the Center for Horticultural Seed Development (Golden Seed Project No. 213007-05-3-CG100) of the Ministry of Agriculture, Food and Rural Affairs (MAFRA), Republic of Korea.

Compliance with ethical standards

All authors read and approved the final draft of the manuscript. The authors confirm that there are no ethical issues in publication of the manuscript.

Conflicts of interest

The authors declared no conflict of interest.

Supplementary material

13313_2019_614_MOESM1_ESM.docx (702 kb)
ESM 1 (DOCX 702 kb)

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Copyright information

© Australasian Plant Pathology Society Inc. 2019

Authors and Affiliations

  • Mehede Hassan Rubel
    • 1
  • Mohammad Rashed Hossain
    • 1
  • Ujjal Kumar Nath
    • 1
  • Sathishkumar Natarajan
    • 1
  • Ji-Hee Lee
    • 2
  • Hee-Jeong Jung
    • 1
  • Hoy-Taek Kim
    • 1
  • Jong-In Park
    • 1
  • Ill-Sup Nou
    • 1
    Email author
  1. 1.Department of HorticultureSunchon National UniversitySuncheonRepublic of Korea
  2. 2.Center for Horticultural Seed Development of Golden Seed Project (GSP)Suncheon National UniversitySuncheonRepublic of Korea

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