Development of a PCR test for detection of Xanthomonas campestris pv. raphani
- 32 Downloads
Leaf spot of Brassica is an important bacterial seed borne disease caused by X. campestris pv. raphani (Xcr), one of the three pathovars of Xanthomonas campestris species which makes significant economic loss throughout the world. A rapid and reliable detection method will be helpful for early diagnosis of the disease. To do that, we designed Xcr specific sequence-characterized amplified region (SCAR) markers from the Xcr specific genomic fragments, identified by aligning the whole genome sequences of several closely related bacterial pathovars and other bacterial species. Our primer set Xcr_59 produced Xcr specific amplicon of 679 bp in PCR assays. This primer pair was also capable of detecting Xcr strains directly from the artificially infected leaf samples prepared by suspending the diseased leaf area in sterile water without extracting bacterial DNA. This rapid, sensitive and reliable detection technique will be useful for adopting any firsthand plant protection measures against the disease.
KeywordsX. campestris pv. raphani campestris pv Raphani Leaf spot PCR marker Brassica
We thank Joana G. Vicente, University of Warwick, UK for providing different strains with known races of Xcc, Xci and Xcr isolates and the Korean Agriculture Culture Collection (KACC), Korea and the International Collection of Microorganisms from Plants (ICMP), New Zealand for providing respective bacterial strains. This work was supported by the Center for Horticultural Seed Development (Golden Seed Project No. 213007-05-3-CG100) of the Ministry of Agriculture, Food and Rural Affairs (MAFRA), Republic of Korea.
Compliance with ethical standards
All authors read and approved the final draft of the manuscript. The authors confirm that there are no ethical issues in publication of the manuscript.
Conflicts of interest
The authors declared no conflict of interest.
- Leu Y, Deng W, Yang W, Wu Y, Cheng A, Hsu S, Tzeng K (2010) Multiplex polymerase chain reaction for simultaneous detection of Xanthomonas campestris pv. campestris and X. campestris pv. raphani. Plant Pathol Bull 2:137–147Google Scholar
- Schaad NW, Postnikova E, Lacy G, Sechler A, Agarkova IV, Stromberg PE, Stromberg VK, Vidaver AM (2006) Emended classification of Xanthomonad pathogens on citrus. Papers in Plant Pathol 96Google Scholar
- White H (1930) Bacterial spot of radish and turnip. Phytopathology 20(8)Google Scholar
- Wu Y, Chen S, Huang S, Cheng A (2007) First report of bacterial leaf spot disease of crucifers caused by Xanthomonas campestris pv. raphani in Taiwan. Plant Pathol Bull 16:87–90Google Scholar