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Pathogenicity of Chrysoporthe deuterocubensis and Myrtoporthe bodenii gen. et sp. nov. on Eucalyptus in Sabah, Malaysia

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Abstract

The Cryphonectriaceae (Diaporthales, Ascomycota) is an important family of fungi including endophytes and pathogens. These fungi cause cankers on trees and can shift hosts between genera and families in the Myrtales. They represent an understudied group in Southeast Asia, where species of Eucalyptus are grown for plantation forestry. Cankers on species of Eucalyptus were surveyed in Sabah, Malaysia, and their causal agents identified. Two species in the Cryphonectriaceae were isolated from Eucalyptus grandis in the Sipitang and Tawau provinces. These fungi were identified based on morphology and a phylogenetic species hypothesis using the internal transcribed spacer regions, the large subunit regions of ribosomal DNA, and the β–tubulin 1 and 2 gene regions. One species identified as Chrysoporthe deuterocubensis is a first report for Malaysia. The other species represented a new taxon, named here as Myrtoporthe bodenii gen. et sp. nov. Pathogenicity tests showed that both Chr. deuterocubensis and M. bodenii were pathogenic to E. grandis and E. deglupta and that M. bodenii is pathogenic to E. pellita. Myrtoporthe bodenii was equally as pathogenic as the well-known canker pathogen Chr. deuterocubensis and it should be regarded as a biosecurity concern.

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Data availability

The two sequence based datasets generated during and/or analysed during the current study are available in TreeBASE (accession no: 24362) at https://www.treebase.org/.

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Acknowledgements

We thank members of the Tree Protection Co-operative Programme (TPCP) and Sabah Softwoods Berhad (SSB) for financial support to undertake this study. Mr. Mahadir Lapammu, Mr. Agustan Alwi and Mr. Manisi Lamuda and their team are also thanked for their assistance in conducting field studies. Mr. Edmund Gan, Mr. Reydzuan Yong, Mr. Jimmy and Mr. Guy Thornton are acknowledged for logistic support. Dr. DongHyeon Lee provided invaluable assistance with data management and analyses. Dr. Runlei Chang and Ms. Ginna Granados provided guidance with laboratory studies and data analyses.

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Correspondence to I. Barnes.

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Rauf, M.R.B.A., McTaggart, A.R., Marincowitz, S. et al. Pathogenicity of Chrysoporthe deuterocubensis and Myrtoporthe bodenii gen. et sp. nov. on Eucalyptus in Sabah, Malaysia. Australasian Plant Pathol. 49, 53–64 (2020). https://doi.org/10.1007/s13313-019-00676-6

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Keywords

  • Cryphonectria canker
  • Diaporthales
  • Eucalyptus
  • Myrtaceae
  • Tree disease