Advertisement

Identification of resistant sources in chilli (Capsicum sp.) genotypes to Groundnut bud necrosis virus (GBNV)

  • B. S. Pavithra
  • K. Madhavi Reddy
  • G. Kedarnath
  • M. Krishna ReddyEmail author
Original Paper
  • 56 Downloads

Abstract

Chilli pepper (Capsicum annuum L.) is an important commercial crop grown on the Indian subcontinent. Recently, its production has been limited due to the incidence of thrips transmitting the Groundnut bud necrosis virus (GBNV). Constraint of such viral pathogens is challenging due to their broad host range and also ineffective chemical means to control the vector populations. The use of resistant cultivars is the most effective and in many cases the only strategy to limit plant viral diseases. In order to search for resistance sources in chilli peppers, a total of 102 Capsicum genotypes were screened for GBNV resistance through mechanical inoculation. Out of the 102 genotypes screened for GBNV resistance, none showed immune reaction, eight genotypes were highly resistant, eight lines were resistant, seven were intermediate resistant and the remaining 79 genotypes showed susceptible reaction on the five-point disease scale. The viral resistance reaction of chilli genotypes was measured based on symptomatology, DAC-ELISA and PCR testing. The highest per cent disease incidence was observed in IC393723 (100%) with an ELISA absorbance (A405) value of 3.03. The lowest per cent disease incidence was observed in eight genotypes viz., EC631810, IIHR4360, IIHR4577, IIHR4578, IIHR4582, IIHR4585, IIHR4587 and IIHR4588 with no symptoms or a few local lesions on the inoculated leaves, with ELISA absorbance values of less than 0.30 and are positive for GBNV RT-PCR testing. These genotypes can be further used in chilli crop improvement programs to develop GBNV resistant cultivars.

Keywords

GBNV Genotypes DAC-ELISA RT-PCR Mechanical Inoculation Tospovirus Symptoms 

Notes

Acknowledgements

The first author thank UGC GOI for providing RGNF fellowship for pursuing Ph.D. in Plant Pathology. This work was carried under the DBT project on ‘Identification and breeding of tospovirus resistance in chillies (Capsicum annuum L.) using molecular markers’. Also, the first author thank Director, Heads of Division of Vegetable Crops and Division of Plant Pathology, ICAR-IIHR, Bengaluru for providing genotypes and laboratory facilities for conducting experiments.

Supplementary material

13313_2019_672_MOESM1_ESM.docx (28 kb)
ESM 1 (DOCX 27 kb)

References

  1. Akram M, Naimuddin (2012) Biological characterization and variability of the nucleocapsid protein gene of Groundnut bud necrosis virus isolates infecting pea. Phytopathol Mediterr 51:266–275Google Scholar
  2. Anjaneya Reddy B, Krishnareddy M, Jalali S, Patil MS, Usha Rani TR (2008) Detection of a tospovirus-infecting tomato (Solanum lycopersicon L.). Indian J Virol 19:1–5Google Scholar
  3. Bera SK, Vinod K, Sunkad G, Rathnakumar AL, Radhakrishnan T (2010a) NRCGCS-86 (INGR 10031) multiple disease resistant Spanish bunch groundnut genotype (resistant to stem rot, late leaf spot, early leaf spot, rust, alternaria leaf blight and PBND). Indian J Pl Genet Resour 24:112Google Scholar
  4. Bera SK, Vinod K, Sunkad G, Rathnakumar AL, Radhakrishnan T (2010b) NRCGCS-85 (INGR 10030) multiple disease resistant Spanish bunch Peanut genotype (resistant to PBND, stem rot, late leaf spot, early leaf spot, alternaria leaf blight and tolerant to rust). Indian J Pl Genet Resour 24:111Google Scholar
  5. Bhat AI, Jain RK, Varma A, Lal SK (2002) Nucleocapsid protein gene sequence studies suggest that soybean bud blight is caused by a strain of Groundnut bud necrosis virus. Curr Sci 82:1389–1392Google Scholar
  6. Black LL, Hobbs HA, Gatti JM (1991) Tomato spotted wilt virus resistance in Capsicum chinense PI152225 and 159236. Plant Dis 75:863CrossRefGoogle Scholar
  7. Boiteux LS, De Avila AC (1994) Inheritance of a resistance specific to tomato spotted wilt tospovirus in Capsicum chinense PI-159236. Euphytica 75:139–142CrossRefGoogle Scholar
  8. Boiteux LS, Nagata T, Dutra WP, Fonseca MEN (1993) Sources of resistance to tomato spotted wilt virus (TSWV) in cultivated and wild species of Capsicum. Euphytica 67:89–94CrossRefGoogle Scholar
  9. Buiel AAM (1993) Resistance in peanut to peanut bud necrosis virus in: durability of disease resistance. Kluwer academic publishers, the Netherlands. Pp. 207-210CrossRefGoogle Scholar
  10. FAOstat (2016) Food and Agricultural Organization of the United Nations Statistical DatabaseGoogle Scholar
  11. Gopal K, Jagadeeshwar G, Prasad Babu Upadhyay HD (2004) Source of resistance to bud necrosis disease R to groundnut. IAN 24:36–38Google Scholar
  12. Gray S, Moyer J (1993) Resistance in Cucumis melo to Watermelon mosaic virus that reduces disease severity and disease incidence. In: Kyle MM (ed) Resistance to Viral Diseases of Vegetables: Genetics and Breeding. Timber Press, Portland, pp 196–216Google Scholar
  13. Hemalatha V, Gangatirkar P, Karande AA, Krishnareddy M, Savithri HS (2008) Monoclonal antibodies to the recombinant nucleocapsid protein of a Groundnut bud necrosis virus infecting tomato in Karnataka and their use in profiling the epitopes of Indian tospovirus isolates. Curr Sci 95:952–957Google Scholar
  14. Hobbs HA, Reddy DVR, Rajeshwari R, Reddy AS (1987) Use of direct antigen coating and protein a coating ELISA procedures for detection of three peanut viruses. Plant Dis 71:747–749CrossRefGoogle Scholar
  15. Jahn M, Paran I, Hoffmann K, Radwanski ER, Livingstone KD, Grube RC, Aftergoot E, Lapidot M, Moyer J (2000) Genetic mapping of the Tsw locus for resistance to the Tospovirus Tomato spotted wilt virus in Capsicum spp. and its relationship to the Sw-5 gene for resistance to the same pathogen in tomato. MPMI 13(6):673–682CrossRefGoogle Scholar
  16. Jain RK, Umamaheswaran K, Bhat AI, Thien HX, Ahlawat YS (2002) Necrosis disease on cowpea, mungbean and tomato is caused by Groundnut bud necrosis virus. Indian Phytopathol 55:354Google Scholar
  17. Kamdar JH, Goswami BR, Bera SK (2014) Genetic molecular diversity in interspecific peanut lines differing in temporal resistance to peanut bud necrosis. African J Biotech 13:385–393CrossRefGoogle Scholar
  18. Krishnareddy M, Usha Rani TR, Kumar A, Madhavireddy K, Pappu HR (2008) First report of Capsicum chlorosis virus (genus Tospovirus) infecting chilli pepper (Capsicum annuum) in India. Plant Dis 92:1469CrossRefGoogle Scholar
  19. Kunkalikar SR, Sudarsana P, Rajagopalan P, Zehr UB, Ravi KS (2010) Biological and molecular characterization of Capsicum chlorosis virus infecting chili and tomato in India. Arch Virol 155:1047–1057CrossRefGoogle Scholar
  20. Kunkalikar SR, Poojari S, Arun BM, Rajagopalan PA, Chen TC, Yeh SD, Naidu RA, Zehr UB, Ravi KS (2011) Importance and genetic diversity of vegetable-infecting tospoviruses in India. Phytopathology 101:367–376CrossRefGoogle Scholar
  21. Lecoq H, Moury B, Desbiez C, Palloix A, Pitrat M (2004) Durable virus resistance in plants through conventional approaches: a challenge. Virus Res 100:31–39CrossRefGoogle Scholar
  22. Mandal B, Jain RK, Krishnareddy M, Kumar NKK, Ravi KS, Pappu HR (2012) Emerging problems of tospoviruses (Bunyaviridae) and their management in the Indian subcontinent. Plant Dis 96(4):468–479CrossRefGoogle Scholar
  23. Des Mcgrath (2007) Capsicum breeding for tospovirus resistance. Horticultural Australia Ltd..Google Scholar
  24. Naik SO, Somashekar (2015) Phenotypic and biochemical mechanism of resistance in groundnut genotype and varieties against leaf hopper and thrips. Eco Env Cons 21(1):535–543Google Scholar
  25. Pappu HR, Jones RAC, Jain RK (2009) Global status of tospovirus epidemics in diverse cropping systems: successes achieved and challenges ahead. Virus Res 141:219–236CrossRefGoogle Scholar
  26. Pavithra BS, Krishnareddy M, Rangaswamy KT (2016) Detection and partial characterization of groundnut bud necrosis virus in chilli. IJSN 7:843–847Google Scholar
  27. Puangmalai P, Potapohn N, Akarapisarn A, Cheewachaiwit S, Insuan N (2013) Screening Capsicum accessions for Tomato Necrotic Ring spot virus resistance. CMU J Nat Sci 12(1):35–42Google Scholar
  28. Ramana CV, Rao PV, Rao RDVJP, Kumar SS, Reddy IP, Reddy YN (2011) Genetic analysis for Peanut bud necrosis virus (PBNV) resistance in tomato (Lycopersicon esculentum mill.). Acta Hortic 914:459–463CrossRefGoogle Scholar
  29. Reddy DVR, Buiel AAM, Satyanarayana T, Dwivedi SL, Reddy AS, Ratna AS, Vijayalakshmi K, Ranga Rao GV, Naidu RA, Wightman JA (1995) Peanut bud necrosis disease: an overview. In: Recent studies on peanut bud necrosis disease: proceedings of a meeting, 20 march 1995. ICRISAT Asia Center, Hyderabad, pp 3–7ppGoogle Scholar
  30. Ruth CH, Subba Rao M, KVM KM, Raghavaiah G, Srinivasa Rao V, Bhaskar Reddy BV (2013) Screening of tomato germplasm against bud necrosis virus disease in tomato GBNV-to. Arch Phytopath Pl Protect 46(1):1–6CrossRefGoogle Scholar
  31. Satyanarayana T, Mitchell SE, Reddy DVR, Brown S, Kresovich S, Jarret R, Naidu RA, Damski JW (1996) Peanut bud necrosis tospovirus S RNA: complete nucleotide sequence, genome organization and homology to other tospoviruses. Arch Virol 141:85–98CrossRefGoogle Scholar
  32. Soler S, Diez M, Roselló S, Nuez F (1999) Movement and distribution of tomato spotted wilt virus in resistant and susceptible accessions of Capsicum spp. Can J Plant Pathol 21:317–325CrossRefGoogle Scholar
  33. Somani AK, Gopal J (2006) Screening of exotic potato germplasm for resistance to stem necrosis. Indian Phytopathol 57:61–64Google Scholar
  34. Srinivasaraghavan A, Gururaj Sunkad SK, Bera MR (2013) Sources of peanut bud necrosis disease resistance in groundnut with desirable yield parameters and pod features. Indian Phytopath 66(2):215–216Google Scholar
  35. Stevens MR, Scott SJ, Gergerich RC (1992) Inheritance of a gene for resistance to Tomato spotted wilt virus (TSWV) from Lycopersicon peruvianum mill. Euphytica 59:9–17Google Scholar
  36. Tamilnayagan T, Suganthy M, Renukadevi P, Malathi VG (2017) Screening of different Germplasm against Groundnut bud necrosis virus (GBNV) and Thrips in tomato. Int J Curr Microbiol App Sci 6(11):2497–2502CrossRefGoogle Scholar
  37. Turina M, Kormelink R, Resende RO (2016) Resistance to Tospoviruses in vegetable crops: epidemiological and molecular aspects. Annu Rev Phytopathol 54:347–371CrossRefGoogle Scholar
  38. Vijayalakshmi K, Wightman JA, Reddy DVR, Ranga Rao GV, Buiel AAM, Reddy DDR (1995) Transmission of Peanut bud necrosis virus by Thrips palmi in India, Pages 179–184 in: Thrips biology and management. B. L. Parker, M. skinner, and T. Lewis, eds. Plenum Press, New YorkGoogle Scholar

Copyright information

© Australasian Plant Pathology Society Inc. 2019

Authors and Affiliations

  • B. S. Pavithra
    • 1
  • K. Madhavi Reddy
    • 2
  • G. Kedarnath
    • 3
  • M. Krishna Reddy
    • 1
    Email author
  1. 1.Division of Plant PathologyICAR-Indian Institute of Horticultural ResearchBengaluruIndia
  2. 2.Division of Vegetable CropsICAR-Indian Institute of Horticultural ResearchBengaluruIndia
  3. 3.Department of Plant PathologyUniversity of Agricultural Sciences, GKVKBengaluruIndia

Personalised recommendations