Identification of resistant sources in chilli (Capsicum sp.) genotypes to Groundnut bud necrosis virus (GBNV)

  • B. S. Pavithra
  • K. Madhavi Reddy
  • G. Kedarnath
  • M. Krishna ReddyEmail author
Original Paper


Chilli pepper (Capsicum annuum L.) is an important commercial crop grown on the Indian subcontinent. Recently, its production has been limited due to the incidence of thrips transmitting the Groundnut bud necrosis virus (GBNV). Constraint of such viral pathogens is challenging due to their broad host range and also ineffective chemical means to control the vector populations. The use of resistant cultivars is the most effective and in many cases the only strategy to limit plant viral diseases. In order to search for resistance sources in chilli peppers, a total of 102 Capsicum genotypes were screened for GBNV resistance through mechanical inoculation. Out of the 102 genotypes screened for GBNV resistance, none showed immune reaction, eight genotypes were highly resistant, eight lines were resistant, seven were intermediate resistant and the remaining 79 genotypes showed susceptible reaction on the five-point disease scale. The viral resistance reaction of chilli genotypes was measured based on symptomatology, DAC-ELISA and PCR testing. The highest per cent disease incidence was observed in IC393723 (100%) with an ELISA absorbance (A405) value of 3.03. The lowest per cent disease incidence was observed in eight genotypes viz., EC631810, IIHR4360, IIHR4577, IIHR4578, IIHR4582, IIHR4585, IIHR4587 and IIHR4588 with no symptoms or a few local lesions on the inoculated leaves, with ELISA absorbance values of less than 0.30 and are positive for GBNV RT-PCR testing. These genotypes can be further used in chilli crop improvement programs to develop GBNV resistant cultivars.


GBNV Genotypes DAC-ELISA RT-PCR Mechanical Inoculation Tospovirus Symptoms 



The first author thank UGC GOI for providing RGNF fellowship for pursuing Ph.D. in Plant Pathology. This work was carried under the DBT project on ‘Identification and breeding of tospovirus resistance in chillies (Capsicum annuum L.) using molecular markers’. Also, the first author thank Director, Heads of Division of Vegetable Crops and Division of Plant Pathology, ICAR-IIHR, Bengaluru for providing genotypes and laboratory facilities for conducting experiments.

Supplementary material

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ESM 1 (DOCX 27 kb)


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Copyright information

© Australasian Plant Pathology Society Inc. 2019

Authors and Affiliations

  • B. S. Pavithra
    • 1
  • K. Madhavi Reddy
    • 2
  • G. Kedarnath
    • 3
  • M. Krishna Reddy
    • 1
    Email author
  1. 1.Division of Plant PathologyICAR-Indian Institute of Horticultural ResearchBengaluruIndia
  2. 2.Division of Vegetable CropsICAR-Indian Institute of Horticultural ResearchBengaluruIndia
  3. 3.Department of Plant PathologyUniversity of Agricultural Sciences, GKVKBengaluruIndia

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