The transcription factor CsBzip10 controls vegetative growth, asexual development, appressorium formation and pathogenicity in the Rosa chinensis anthracnose fungus Colletotrichum siamense
- 12 Downloads
Transcription factors play crucial roles in development and virulence in a variety of pathogens. Here, we identified and characterized the transcription factor CsBzip10 in Colletotrichum siamense. CsBZIP10 encodes a 316-amino acid protein with a conserved bZIP-ATF2 domain, which is an ortholog of MoBzip10 in the rice blast fungus Magnaporthe oryzae. Targeted deletion of CsBZIP10 revealed that vegetative growth, conidiation and appressorium formation of the ΔCsbzip10 mutant were significantly reduced. The ΔCsbzip10 mutant had decreased sensitivity to the cell wall disturbing agent SDS and oxidative stress (H2O2), and increased sensitivity to osmotic stress (NaCl and KCl). Virulence and infectious hyphal growth of the ΔCsbzip10 mutant was remarkably decreased on Rosa chinensis leaves. CsBzip10 is a key transcription factor, that plays pleiotropic roles in the evelopment and pathogenicity of the R. chinensis anthracnose fungus C. siamense.
KeywordsTranscription factor Vegetative growth Asexual development Appressorium formation Pathogenicity
This work was supported by grants from National Natural Science Foundation of China (Grant number: 31570641).
- Fan XW (2018) A brief history of Chinese rose communication: from Slater’s China rose to Chinese red. J ZhongZhou Univ 35:86–89Google Scholar
- Fang YL, Xia LM, Wang P, Zhu LH, Ye JR, Huang L (2018) The MAPKKK CgMck1 is required for cell wall integrity, appressorium development, and pathogenicity in Colletotrichum gloeosporioides. Genes-Basel 9. https://doi.org/10.3390/Genes9110543
- Feng FS (2019) First report of Colletotrichum siamense causing anthracnose on Rosa chinensis in China. Plant Dis. https://doi.org/10.1094/PDIS-11-18-1966-PDN
- Guo M, Guo W, Chen Y, Dong SM, Zhang X, Zhang HF, Song WW, Wang W, Wang Q, Lv RL, Zhang ZG, Wang YC, Zheng XB (2010) The basic leucine zipper transcription factor Moatf1 mediates oxidative stress responses and is necessary for full virulence of the rice blast fungus Magnaporthe oryzae. Mol Plant Microbe In 23:1053–1068CrossRefGoogle Scholar
- Guo M, Chen Y, Du Y, Dong YH, Guo W, Zhai S, Zhang HF, Dong SM, Zhang ZG, Wang YC, Wang P, Zheng XB (2011) The bZIP transcription factor MoAP1 mediates the oxidative stress response and is critical for pathogenicity of the rice blast fungus Magnaporthe oryzae. PLoS Pathog 7:e1001302CrossRefPubMedCentralGoogle Scholar
- Photita W, Lumyong S, Lumyong P, McKenzie EHC, Hyde KD (2004) Are some endophytes of Musa acuminata latent pathogens? Fungal Divers 16:131–140Google Scholar