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Effective detection of Lily Symptomless Virus using the reverse transcription loop–mediated isothermal amplification method

  • Xiao Zhao
  • Yanan Du
  • Yongchun Zhang
  • Jieling Liang
  • Youming Cai
  • Yan Xu
  • Xiaorui Fan
  • Wenjing Wu
  • Qi Zhang
  • Xiaoxia Zhang
  • Dan Yang
  • Ziping Zhu
  • Huanzhen Zhao
  • Wanjing Zhang
  • Kai ZhaoEmail author
Research Note
  • 11 Downloads

Abstract

Lily Symptomless Virus (LSV) is prevalent in lily, causing severe economic losses in Lily production. Therefore, it is quite necessary to develop an effective method for detecting LSV. This study aimed to develop a visual Reverse Transcription Loop–Mediated Isothermal Amplification (RT-LAMP) method to detect LSV. The optimum condition was 60 min at 65 °C. The primers designed were highly specific to LSV and did not cross-react with other seven Lily viruses. The sensitivity of RT-LAMP assay for LSV was 0.0001 ng/ul. This RT-LAMP method was rapid, simple, highly specific, and sensitive and helped in the reliable visual detection of LSV in Lily plants.

Keywords

RT-LAMP Lily Carlavirus Virus detection 

Notes

Acknowledgements

Thisrogram, China (Grant No.G2014070203) and agriculture research system of Shanghai, China (Grant No. 201708). These funding bodies had no role in design, collection, and analysis of t work was financially supported by Shanghai agriculture applied technology development phe study and preparation of the manuscript.

References

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Copyright information

© Australasian Plant Pathology Society Inc. 2019

Authors and Affiliations

  • Xiao Zhao
    • 1
  • Yanan Du
    • 2
  • Yongchun Zhang
    • 1
  • Jieling Liang
    • 3
  • Youming Cai
    • 1
  • Yan Xu
    • 2
  • Xiaorui Fan
    • 2
  • Wenjing Wu
    • 2
  • Qi Zhang
    • 2
  • Xiaoxia Zhang
    • 2
  • Dan Yang
    • 3
  • Ziping Zhu
    • 3
  • Huanzhen Zhao
    • 1
  • Wanjing Zhang
    • 2
  • Kai Zhao
    • 1
    • 4
    Email author
  1. 1.Shanghai Academy of Agricultural SciencesShanghaiChina
  2. 2.College of Life and Environment SciencesShanghai Normal UniversityShanghaiChina
  3. 3.School of Life ScienceTaizhou UniversityTaizhouChina
  4. 4.Key Laboratory of Agricultural Genetics and BreedingShanghai Academy of Agricultural SciencesShanghaiChina

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