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Elimination of Apple stem pitting virus from in vitro-cultured pear by an antiviral agent combined with thermotherapy

  • G. J. Hu
  • N. Hong
  • G. P. Wang
Research Note
  • 25 Downloads

Abstract

Six in vitro pear cultivars, Wonhwang, Xuehua, Conference, Stankimson, Starcrimson and Red Bastlett were infected by Apple stem pitting virus (ASPV) and were treated by combination of 25 μg/ml ribavirin and temperature 35 °C. Results showed that ribavirin could enhance the proliferation of Xuehua, Conference, Stankimson and Starcrimson and death plants were found in Xuehua and Red Bastlett and the total survival rate of the six cultivars was 93.5%. After treatment, the regenerated plants were detected by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that ASPV in all regenerated plants could not be detected and the total elimination rate of the six cultivars was 92.3%. The combination showed high efficacy on elimination of ASPV.

Keywords

Pear In vitro culture Apple stem pitting virus (ASPV) Chemotherapy Thermotherapy Virus elimination 

Notes

Acknowledgements

This work is supported by the China Agriculture Research System CARS-29-10.

Supplementary material

13313_2018_606_MOESM1_ESM.docx (17 kb)
ESM 1 (DOCX 16 kb)

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Copyright information

© Australasian Plant Pathology Society Inc. 2018
corrected publication December/2018

Authors and Affiliations

  1. 1.National Key Laboratory of Agromicrobiology, College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.National Center for Eliminating Viruses from Deciduous Fruit Tree, Research Institute of PomologyChinese Academy of Agricultural SciencesXingchengPeople’s Republic of China

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