Australasian Plant Pathology

, Volume 47, Issue 2, pp 181–188 | Cite as

Previously unrecognized diversity within fungal fruit rot pathosystems on Vitis vinifera and hybrid white wine grapes in Mid-Atlantic vineyards

Original Paper
  • 73 Downloads

Abstract

Fruit rot diseases are among the most economically important challenges facing the rapidly growing wine grape industry in the Mid-Atlantic region of the United States. Although diverse fungi are associated with fruit rots, most are considered secondary pathogens. Surveys in fall 2015 and 2016 recovered seven species of hyphal fungi from diseased berries, in which Aspergillus japonicus and Colletotrichum fioriniae were the dominant species recovered from Vidal blanc clusters, and Alternaria alternata and Colletotrichum fioriniae were the most common species recovered from Chardonnay. Two species not previously described as pathogens, Aspergillus japonicus and Pestalotiopsis telopeae, could initiate fruit rot in intact Chardonnay berries (incidence: 5–17.5% of berries); all other species were weak pathogens and likely function as secondary colonists. Introduction of inoculum into a mechanical wound significantly increased disease incidence for all species (P < 0.001). The hybrid white cultivar Vidal blanc, grown primarily for fruit rot-resistant traits, was found to be highly susceptible to the newly described pathogen A. japonicus, as well as the ripe rot pathogen C. fioriniae (P = 0.01). This is one of the first studies to evaluate fruit rot pathosystem diversity in Mid-Atlantic vineyards, and to assess the relative susceptibly of a hybrid cultivar to fruit rot diseases. This information will be used to develop diagnostic resources and to further research and outreach efforts for improving fungal fruit rot management.

Keywords

Chardonnay Vidal blanc Pestalotiopsis Aspergillus Alternaria Maryland 

Notes

Acknowledgements

The authors would like to thank Ben Beale, GR Welsh, and Joe Fiola for their assistance in fruit rot collections, project guidance, and for providing grapes for trials, Beth Hellman for assistance in sequence analysis, Kate Everts, Kelly Hamby, Tony Wolf, and Mizhuo Nita for project feedback, and both Emmi Koivunen and Rino Oguchi for editorial assistance. Support for this project was provided by the University of Maryland, College Park (start-up funds).

References

  1. Aegerter BJ, Gordon TR (2006) Rates of pitch canker induced seedling mortality among Pinus radiata families varying in levels of genetic resistance to Gibberella circinata (anamorph Fusarium circinatum). For Ecol Man 235(1-3):14–17.  https://doi.org/10.1016/j.foreco.2006.07.011 CrossRefGoogle Scholar
  2. Anonymous (2010) Economic impact study of the Maryland wine industry. Maryland Wineries Association. University of Baltimore. http://www.mbbwl.org/files/md_wine_industry_2009_impact_study.pdf. Accessed 19 Jan 2016
  3. Anonymous (2015) The 2014 Maryland vineyard survey. Maryland Grape Growers Association. Web. http://www.marylandgrapes.org/Anonymous. Accessed 19 Jan 2016
  4. Anonymous (2016a) 2015 State Agricultural Overview, Pennsylvania. https://www.nass.usda.gov/Quick_Stats/Ag_Overview/stateOverview.php?state=pennsylvania. Accessed 2 Nov 2016
  5. Anonymous (2016b) 2015 State Agricultural Overview, Virginia. https://www.nass.usda.gov/Quick_Stats/Ag_Overview/stateOverview.php?state=VIRGINIA. Accessed 2 Nov 2016
  6. Barata A, Malfeito-Ferreira M, Loureiro V (2012) The microbial ecology of wine grape berries. Int J Food Micro 153(3):243–259.  https://doi.org/10.1016/j.ijfoodmicro.2011.11.025 CrossRefGoogle Scholar
  7. Barnett HL, Hunter BB (1998) Illustrated genera of imperfect fungi. Vol. 3340. St. Paul: APS PressGoogle Scholar
  8. Baudoin A (2012) Survey of fungicide resistance of Botrytis cinerea in Virginia vineyards. Phytopathology 103:6Google Scholar
  9. Briceño EX, Latorre BA (2008) Characterization of Cladosporium rot in grapevines, a problem of growing importance in Chile. Plant Dis 92(12):1635–1642.  https://doi.org/10.1094/PDIS-92-12-1635 CrossRefGoogle Scholar
  10. Cozzi G, Haidukowski M, Perrone G, Visonti A, Logrieco A (2009) Influence of Lobesia botrana field control on black Aspergilli rot and ochratoxin a contamination in grapes. J Food Prot 72(4):894–897.  https://doi.org/10.4315/0362-028X-72.4.894 CrossRefPubMedGoogle Scholar
  11. Deng JX, Sang HK, Yong SH, Lim BS, SH Y (2013) Postharvest fruit caused by Pestalotiopsis sp. on grape in Korea. Aust Plant Dis Notes 8(1):111–114.  https://doi.org/10.1007/s13314-013-0109-7 CrossRefGoogle Scholar
  12. Fermaud M, Le Menn R (1992) Transmission of Botrytis cinerea to grapes by grape berry moth larvae. Phytopathology 82(12):1393–1398.  https://doi.org/10.1094/Phyto-82-1393 CrossRefGoogle Scholar
  13. Gebhardt K, Anderson AM, Kirkpatrick KN, Shwiff SA (2011) A review and synthesis of bird and rodent damage estimates to select California crops. Crop Prot 30(9):1109–1116.  https://doi.org/10.1016/j.cropro.2011.05.015 CrossRefGoogle Scholar
  14. Greer LA, Harper JD, Steel CC (2014) Infection of Vitis vinifera (cv chardonnay) inflorescences by Colletotrichum acutatum and Greenaria uvicola. J Phytopathol 162(6):407–410.  https://doi.org/10.1111/jph.12201 CrossRefGoogle Scholar
  15. Hall M, Loeb G, Wilcox W (2015) Etiology and management of sour rot in grapes. Phytopathology 105(S4):56–56Google Scholar
  16. Hed B, Ngugi HK, Travis JW (2014) Short-and long-term effects of leaf removal and gibberellin on chardonnay grapes in the Lake Erie region of Pennsylvania. Am J Enol Vitic 66(1):22–29.  https://doi.org/10.5344/ajev.2014.14034 CrossRefGoogle Scholar
  17. Jayawardena RS, Zhang W, Liu M, Maharachchikumbura S, Zhou Y, Huang J, Nilthong S, Wang Z, Li X, Yan J, Hyde KD (2015) Identification and characterization of Pestalotiopsis-like fungi related to grapevine diseases in China. Fung Biol 119(5):348–361.  https://doi.org/10.1016/j.funbio.2014.11.001 CrossRefGoogle Scholar
  18. Kepner C (2016) Understanding late season fruit rot pathosystems and insect interactions in mid-Atlantic vineyards. University of Maryland, College Park, MS thesis, Maryland, USAGoogle Scholar
  19. Kou LP, Gaskins V, Luo YG, Jurick WM II (2015) First report of Colletotrichum fioriniae causing postharvest decay on ‘Nittany’ apple fruit in the United States. Genet Resour Crop Evol 62(7):765–794.  https://doi.org/10.1094/PDIS-08-13-0816-PDN Google Scholar
  20. Larena I, Salazar O, González V, Julián MC, Rubio V (1999) Design of a primer for ribosomal DNA internal transcribed spacer with enhanced specificity for ascomycetes. J Biotechnol 75(2):187–194.  https://doi.org/10.1016/S0168-1656(99)00154-6 CrossRefPubMedGoogle Scholar
  21. Leong SL, Hocking AD, Scott ES (2006) Survival and growth of Aspergillus carbonarius on wine grapes before harvest. Int J Food Micro 111:S83–S87.  https://doi.org/10.1016/j.ijfoodmicro.2006.03.005 CrossRefGoogle Scholar
  22. Maharachchikumbura SSN, Guo L, Chukeatirote E, Bahkali AH, Hyde K (2011) Pestalotiopsis- morphology, phylogeny, biochemistry, and diversity. Fung Div 50:167–187.  https://doi.org/10.1007/s13225-011-0125-x CrossRefGoogle Scholar
  23. Nita M, Oliver C, Hartley S (2014) Screening of fungicides for the control of ripe rot on grapes caused by Colletotrichum acutatum and C. gloeosporioides. Phytopathology 104(11):85–86.  https://doi.org/10.1111/j.1755-0238.2011.00143.x Google Scholar
  24. O’Donnell K, Kistler HC, Cigelnik E, Ploetz RC (1998) Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc Nat Acad Sci 95(5):2044–2049.  https://doi.org/10.1073/pnas.95.5.2044 CrossRefPubMedPubMedCentralGoogle Scholar
  25. Pscheidt JW, Pearson RC (1989) Time of infection and control of Phomopsis fruit rot of grape. Plant Dis 73(10):829–833.  https://doi.org/10.1094/PD-73-0829 CrossRefGoogle Scholar
  26. Reisch BL, Pool RM, Peterson DV, Martens M, Henick-Kling T (1993) Wine and grape juice varieties for cool climates. Cornell Coop Ext Bulletin 233:13Google Scholar
  27. Rooney-Latham S, Janousek CN, Eskalen A, Gubler WD (2008) First report of Aspergillus carbonarius causing sour rot of table grapes (Vitis vinifera) in California. Plant Dis 92(4):651.  https://doi.org/10.1094/PDIS-92-4-0651A CrossRefGoogle Scholar
  28. Rousseaux S, Diguta CF, Radoi-Matei F, Alexandre H, Guilloux-Benatier M (2014) Non-Botrytis grape-rotting fungi responsible for earthy and moldy off-flavors and mycotoxins. Food Micro 38:104–121.  https://doi.org/10.1016/j.fm.2013.08.013 CrossRefGoogle Scholar
  29. Sergeeva V, Priest M, Nair NG (2005) Species of Pestalotiopsis and related genera occurring on grapevines in Australia. Aust Plant Path 34(2):255–258.  https://doi.org/10.1071/AP05009 CrossRefGoogle Scholar
  30. Steel CC, Blackman JW, Schmidtke LM (2013) Grapevine bunch rots: impacts on wine composition, quality, and potential procedures for the removal of wine faults. J Ag Food Chem 61(22):5189–5206.  https://doi.org/10.1021/jf400641r CrossRefGoogle Scholar
  31. Swett CL, Bourret T, Gubler WD (2016) Characterizing the brown spot pathosystem in late-harvest table grapes (Vitis vinifera L.) in the California central valley. Plant Dis 100(11):2204–2210.  https://doi.org/10.1094/PDIS-11-15-1343-RE CrossRefGoogle Scholar
  32. Urbez-Torres JR, Peduto F, Striegler RK, Urrea-Romero KE, Rupe JC, Cartwright RD, Gubler WD (2012) Characterization of fungal pathogens associated with grapevine trunk diseases in Arkansas and Missouri. Fung Div 52(1):169–189.  https://doi.org/10.1007/s13225-011-0110-4 CrossRefGoogle Scholar
  33. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322Google Scholar
  34. Wilcox WF, Gubler WD, Uyemoto JK (2015) Compendium of grape diseases, disorders, and pests, vol 2. APS Press, St PaulGoogle Scholar
  35. Xu L, Kusakari S, Hosomi A, Toyoda H, Ouchi S (1999) Postharvest diseases of grapes caused by Pestalotiopsis species. Ann Phytopath Soc Japan 65(3):305–311.  https://doi.org/10.1007/s13314-013-0109-7 CrossRefGoogle Scholar
  36. Zoecklein BW, Wolf TK, Duncan NW, Judge JM, Cook MK (1992) Effects of fruit zone leaf removal on yield, fruit composition, and fruit rot incidence of chardonnay and white Riesling (Vitis vinifera L.) grapes. Am J Enol Vitic 43:139–148Google Scholar

Copyright information

© Australasian Plant Pathology Society Inc. 2018

Authors and Affiliations

  1. 1.Department of Plant Science and Landscape ArchitectureUniversity of MarylandCollege ParkUSA
  2. 2.Department of Plant PathologyUniversity of CaliforniaDavisUSA

Personalised recommendations