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Molecular & Cellular Toxicology

, Volume 15, Issue 1, pp 85–92 | Cite as

Regulatory role of Wdr24 in autophagy activity during zebrafish embryogenesis

  • Yong-Il Kim
  • In-Koo Nam
  • Jae-Young Um
  • Seong-Kyu Choe
Original Paper
  • 11 Downloads

Abstract

Backgrounds

TOR and autophagy are essential pathways to mediate anabolic and catabolic reactions, respectively, in response to various nutritional stimuli. Vertebrate development requires such reactions to achieve the common goal of generating an independent organism from a single fertilized egg.

Methods

Using the zebrafish as an animal model, we characterized the role of Wdr24, a component of the GATOR2 complex that reportedly activates TORC1.

Results

Sequence analysis and subcellular localization of zebrafish Wdr24 suggested functional resemblance to its mammalian counterpart. We found that wdr24 expression commences during early embryogenesis, implicating its requirement. Accordingly, wdr24 knockdown induced defective embryogenesis accompanied by massive cell death. The developmental defects induced by wdr24 knockdown were attributable, at least in part, to dysregulated autophagy, which could be partially restored by wdr24 overexpression.

Conclusion

These findings suggest that a conserved role of Wdr24 may be a critical part of the cellular metabolism in different species.

Keywords

Wdr24 GATOR2 TORC1 Autophagy Zebrafish 

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Copyright information

© The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Nature B.V. 2019

Authors and Affiliations

  • Yong-Il Kim
    • 1
  • In-Koo Nam
    • 2
  • Jae-Young Um
    • 3
  • Seong-Kyu Choe
    • 1
  1. 1.Department of MicrobiologyWonkwang University School of MedicineIksanRepublic of Korea
  2. 2.Department of Biomedical Science & Engineering, Institute of Integrated TechnologyGwangju Institute of Science & TechnologyGwangjuRepublic of Korea
  3. 3.College of Korean Medicine, Basic Research Laboratory for Comorbidity Regulation, Graduate SchoolKyung Hee UniversitySeoulRepublic of Korea

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