A toxicogenomics study of two chemicals in coffee roasting process
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Roasted coffee beans are broadly consumed in nearly all classes of the population. 2,3-Butanedione is a food flavor that causes obstructive bronchiolitis in microwave popcorn manufacturing workers. It can be naturally produced when coffee is roasted.
To determine effects of 2,3-butanedione (diacetyl) and 2,3-pentanedione on workers’ health during the coffee roasting process, we performed a toxicogenomics study for differentially expressed genes in lung cells after exposure to the two chemicals.
Gene selection was performed by clustering, gene ontology/pathway, and data mining using microarray data. Target microRNAs and genes were selected based on the expression of microRNAs and correlation analysis of genes and microRNAs. As a result of expression analysis of target gene miRNAs affected by the two chemicals, it was evaluated that Fosl1, Rb1, Aspn, Dusp1, Rnf19b, Jun, and Hbegf were over-regulated by targeting down-regulated miRNAs mutated by two chemicals. Using OMIM database and KEGG pathway, we found that Terc and Bmpr2 were two changed genes by matching with the KEGG disease pathway database.
Dosage, duration, exposure, and extrapolations are necessary to reflect effects of diacetyl and 2,3-pentanedione on workers in the coffee roasting process. This study focuses on early biochemical changes, mechanisms, and early biomarker discovery when normal lung cells are exposed to these two chemicals.
Keywords2,3-Butanedione Coffee Gene expression profile 2,3-Pentanedione Roasting
This study was supported by the Korean Occupational Safety and Health Agency (Ulsan, Republic of Korea), the Ministry of Employment and Labor (Sejong, Republic of Korea), and a Grant-in Aid for chemical research (2018).
KTR designed the experiments and analyzed the results. SJK conducted the experiments and analyzed the raw data. All authors contributed to writing of the manuscript.
Compliance with ethical standards
Conflict of interest
Kyung-Taek Rim and Soo-Jin Kim declare that they have no conflicts of interest regarding the contents of this article.
The article does not contain any studies with human participants or animals performed by any of the authors, and it has been carried out in accordance with the institutional and national guidelines.
- American Conference of Governmental Industrial Hygienists (2016) 2016 TLVs and BEIs. Cincinnati, OH, pp 2–24Google Scholar
- Centers for Disease Control and Prevention (CDC) (2007) Chemical information review document for artificial butter flavoring and constituents diacetyl [CAS No. 431-03-8] and acetoin [CAS No. 513-86-0] supporting nomination for toxicological evaluation by the national toxicology program January. https://search.cdc.gov/search/?query=coffee+roasting&sitelimit=&utf8=%E2%9C%93&affiliate=cdc-main
- Chen J, Hu C, Pan P (2017) Extracellular vesicle MicroRNA transfer in lung diseases. Front Physiol 8:1028 (Published online) Google Scholar
- Coffee TV (2016) Status of franchise café, vol 1. nationwide. http://coffeetv.co.kr
- Conickx G, Cobos FA, van den Berge M, Faiz A, Timens W, Hiemstra PS, Joos GF, Brusselle GG, Mestdagh P, Bracke KR (2017) microRNA profiling in lung tissue and bronchoalveolar lavage of cigarette smoke-exposed mice and in COPD patients: a translational approach. Sci Rep 7:12871Google Scholar
- George RB (2005) Chest medicine: essentials of pulmonary and critical care medicine, 5th edn. Lippincott Williams & Wilkins, Philadelphia, p 172Google Scholar
- Han YH et al (2017) Estimation of air pollutant emissions from coffee roasting. In: Proceedings of conference in Korean society of atmospheric environment, vol 168Google Scholar
- Health and Safety Executive (2013) Control of exposure to grain dust: an employee’s guideGoogle Scholar
- Kumar J, Das S, Teoh SL (2018) Dietary Acrylamide and the risks of developing cancer: facts to ponder. Front Nutr 5:14Google Scholar
- Lewis RJ, Sr. (ed) (2004) Sax’s dangerous properties of industrial materials. Skin, eye and respiratory irritations: a skin irritant, 11th edn. Wiley, Hoboken, pp 586–2844Google Scholar
- Lim JH et al (2017) Measurement of air pollutant emission in coffee roaster. In: Proceedings of conference in Korean society of atmospheric environment, p 168Google Scholar
- National Toxicology Program (NTP) (2014). https://ntpsearch.niehs.nih.gov/?query=coffee+roasting
- NIOSH (2019) NOES survey 1981–1983Google Scholar
- Rabe KF, Hurd S, Anzueto A, Barnes PJ, Buist SA, Calverley P, Fukuchi Y, Jenkins C, Rodriguez-Roisin R, van Weel C, Zielinski J (2007) Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease: GOLD executive summary. Am J Respir Crit Care Med 176(6):532–555CrossRefGoogle Scholar
- Reilly JJ, Silverman EK, Shapiro SD (2011) Chronic obstructive pulmonary disease. In: Longo D, Fauci A, Kasper D, Hauser S, Jameson J, Loscalzo J (eds) Harrison’s principles of internal medicine, 18th edn, McGraw Hill, pp 2151–9Google Scholar
- Research Institute for Health and Environment (2012) Incheon Metropolitan City, Survey of benzopyrene content in coffee beans. http://english.incheon.go.kr/index.do
- Richter DC, Joubert JR, Nell H, Schuurmans MM, Irusen EM (2008) Diagnostic value of post-bronchodilator pulmonary function testing to distinguish between stable, moderate to severe COPD and asthma. Int J Chron Obstruct Pulmon Dis 3(4):693–699Google Scholar
- Smith BK, Timby NE (2005) Essentials of nursing: care of adults and children. Lippincott Williams & Wilkins, Philadelphia, p 338Google Scholar
- van Rooy FGBGJ, Smit LAM, Houba R, Zaat VAC, Rooyackers JM, Heederik DJJ (2009) A cross-sectional study of lung function and respiratory symptoms among chemical workers producing diacetyl for food flavourings. Occup Environ Med 66:105–110Google Scholar
- Vestbo J (2013) Definition and overview: global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease. Global Initiative for Chronic Obstructive Lung Disease, pp 1–7Google Scholar
- World Health Organization (2013) The 10 leading causes of death in the world, 2000 and 2011Google Scholar
- Zaccone EJ, Goldsmith WT, Shimko MJ, Wells JR, Schwegler-Berry D, Willard PA, Case SL, Thompson JA, Fedan JS (2015) Diacetyl and 2,3-pentanedione exposure of human cultured airway epithelial cells: Ion transport effects and metabolism of butter flavoring agents. Toxicol Appl Pharmacol 289:542–549CrossRefGoogle Scholar