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A transcriptome analysis uncovers Panax notoginseng resistance to Fusarium solani induced by methyl jasmonate

  • Diqiu LiuEmail author
  • Qin Zhao
  • Xiuming Cui
  • Rui Chen
  • Xin Li
  • Bingling Qiu
  • Feng Ge
Research Article

Abstract

Background

Panax notoginseng is a famous Chinese herbal medicine, but the root rot disease mainly caused by Fusarium solani severely reduces the yield and quality of its medicinal materials.

Objective

The defense priming in P. notoginseng through exogenous application of signaling molecule will supply theoretical support for the exogenous regulation of disease resistance in P. notoginseng.

Methods

In this study, the exogenous application of methyl jasmonate (MeJA) increased P. notoginseng’s resistance to F. solani. Furthermore, the P. notoginseng transcriptome during F. solani infection was investigated through next-generation sequencing to uncover the resistance mechanism of P. notogingseng induced by MeJA.

Results

The de novo assembly of transcriptome sequences produced 80,551 unigenes, and 36,771 of these unigenes were annotated by at least one database. A differentially expressed gene analysis revealed that a large number of genes related to terpenoid backbone biosynthesis, phenylalanine metabolism, and plant–pathogen interactions were predominantly up-regulated by MeJA. Moreover, jasmonic acid (JA) biosynthesis-related genes and the JA signaling pathway genes, such as linoleate 13S-lipoxygenase, allene oxide cyclase, allene oxide synthase, TIFY, defensin, and pathogenesis-related proteins, showed increased transcriptional levels after inoculation with F. solani. Notably, according to the gene expression analysis, JA and ethylene signaling pathways may act synergistically to positively regulate the defense responses of P. notoginseng to F. solani.

Conclusion

JA signaling appears to play a vital role in P. notoginseng responses to F. solani infection, which will be helpful in improving the disease resistance of P. notoginseng cultivars as well as in developing an environmentally friendly biological control method for root rot disease.

Keywords

Panax notoginseng Transcriptome Methyl jasmonate Fusarium solani Differentially expressed genes 

Notes

Acknowledgements

This work was financially supported by a grant received from the National Natural Sciences Foundation of China (81560610). We thank Lesley Benyon, PhD, from Liwen Bianji, Edanz Group China, for editing the English text of a draft of this manuscript.

Supplementary material

13258_2019_865_MOESM1_ESM.tif (1009 kb)
Fig. S1 The diagrammatic sketch of the MeJA treatment and F. solani inoculation (TIFF 1008 kb)
13258_2019_865_MOESM2_ESM.tif (180 kb)
Fig. S2 Statistics of de novo assembly of P. notoginseng transcriptome. a, Distribution of length of all contigs. b, Distribution of length of all unigenes (TIFF 180 kb)
13258_2019_865_MOESM3_ESM.tif (686 kb)
Fig. S3 A bar plot describing the gene expression levels with FPKM (Fragments Per Kb per Million reads) value. CK (W), P. notoginseng roots pretreated with sterile water; CK (M), P. notoginseng roots pretreated with MeJA; 4 h (W), 4 hpi (hours post inoculation with F. solani) for the sample CK (W); 4 h (M), 4 hpi for the sample CK (M); 12 h (W), 12 hpi for the sample CK (W); 12 h (M), 12 hpi for the sample CK (M); 24 h (W), 24 hpi for the sample CK (W); 24 h (M), 24 hpi for the sample CK (M); 48 h (W), 48 hpi for the sample CK (W); 48 h (M), 48 hpi for the sample CK (M); 72 h (W), 72 hpi for the sample CK (M); 72 h (M), 72 hpi for the sample CK (M) (TIFF 686 kb)
13258_2019_865_MOESM4_ESM.tif (676 kb)
Fig. S4 The KEGG analysis of differentially expressed genes (DEGs) in P. notoginseng roots during F. solani infection. CK (W), P. notoginseng roots treated with sterile water; CK (M), P. notoginseng roots treated with MeJA; 4 h (W), 4 hpi (hours post inoculation with F. solani) for the sample CK (W); 4 h (M), 4 hpi for the sample CK (M); 12 h (W), 12 hpi for the sample CK (W); 12 h (M), 12 hpi for the sample CK (M); 24 h (W), 24 hpi for the sample CK (W); 24 h (M), 24 hpi for the sample CK (M); 48 h (W), 48 hpi for the sample CK (W); 48 h (M), 48 hpi for the sample CK (M); 72 h (W), 72 hpi for the sample CK (M); 72 h (M), 72 hpi for the sample CK (M) 4 (TIFF 675 kb)
13258_2019_865_MOESM5_ESM.docx (22 kb)
Supplementary material 5 (DOCX 21 kb)

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Copyright information

© The Genetics Society of Korea 2019

Authors and Affiliations

  • Diqiu Liu
    • 1
    • 2
    Email author
  • Qin Zhao
    • 1
    • 2
  • Xiuming Cui
    • 1
    • 2
  • Rui Chen
    • 1
    • 2
  • Xin Li
    • 1
    • 2
  • Bingling Qiu
    • 1
    • 2
  • Feng Ge
    • 1
    • 2
  1. 1.Faculty of Life Science and TechnologyKunming University of Science and TechnologyKunmingChina
  2. 2.Yunnan Provincial Key Laboratory of Panax NotoginsengKunmingChina

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