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Genes & Genomics

, Volume 41, Issue 11, pp 1341–1355 | Cite as

Genome-wide characterization of the NUCLEAR FACTOR-Y (NF-Y) family in Citrus grandis identified CgNF-YB9 involved in the fructose and glucose accumulation

  • Yiting Mai
  • Lanya Shui
  • Kaisen Huo
  • Jun NiuEmail author
Research Article
  • 71 Downloads

Abstract

Background

Nuclear factor Y (NF-Y) is increasingly known to be involved in many aspects of plant growth and development. To date, the systematic characterization of NF-Y family has never been reported in Citrus grandis.

Objective

Genome-wide characterization of C. grandis NF-Y (CgNF-Y) family and analysis of their role in sucrose metabolism.

Methods

NF-Y conserved models were employed to identify CgNF-Y genes from genomic data. Phylogenetic tree was generated by the neighbor-joining method using program MEGA 7.0. Based on our previous transcriptomic data, the transcription levels were calculated by RSEM software and were clustered by ShortTime-series Expression Miner. The plant expression vector of CgNF-YB9 was constructed using In-Fusion Cloning and transferred into tobacco by leaf disc transformation method. Soluble sugars and gene expressions were analysis by HPLC and qRT-PCR, respectively.

Results

A total of 24 CgNF-Y genes (6 CgNF-YAs, 13 CgNF-YBs and 5 CgNF-YCs) were identified with conserved domains. Phylogenetic analysis of the NF-Y proteins indicated that NF-YA, NF-YB and NF-YC could be categorized into four, five and three clades, respectively. Expression profiling analysis reflected spatio-temporally distinct expression patterns for CgNF-Y genes. Importantly, we observed a positive correlation between the expression level of CgNF-YB9 and the content of soluble sugar. Moreover, CgNF-YB9-corelated genes were enriched in carbohydrate metabolism. In CgNF-YB9 overexpression lines, sucrose content showed a decrease, whereas glucose and fructose contents displayed an increase. As expected, the transcription levels of sucrose-phosphate synthase and vacuolar invertase in transgenic Line 3 were observed with significantly down- and up-regulated, respectively.

Conclusions

The structure, phylogenetic relationship and expression pattern of 24 CgNF-Y genes were identified, and CgNF-YB9 was involved in sucrose metabolism.

Keywords

Citrus grandis NF-Y family CgNF-YB9 Sucrose metabolism 

Abbreviations

DAF

Days after flowering

FPKM

Fragment per kilobase of exon model per million mapped reads

GO

Gene Ontology

HPLC

High-performance liquid chromatography

HFM

Histone-fold motif

INV

Invertase

LEC1

LEAFY COTYLEDON1

KEGG

Kyoto Encyclopedia of Genes and Genomes

MEME

Multiple Em for Motif Elicitation

NF-Y

Nuclear factor Y

PJS

Pummelo juice sacs

SPP

Sucorse-phosphate phosphatase

SPS

Sucrose-phosphate synthase

SUS

Sucrose synthase

VIN

Vacuolar invertase

WT

Wild tobacco

Notes

Acknowledgements

This research was supported by Natural Science Foundation of Hainan Province (317045) and Hainan university research funded projects (KYQD(ZR)1701).

Author contributions

JN conceived and designed the experiments. YM and LS wrote the manuscript and performed the analysis. KH conducted the transgenosis and detection.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13258_2019_862_MOESM1_ESM.docx (21 kb)
Supplementary material 1 (DOCX 20 kb) Supplementary Table S1 The primers for qRT-PCR analysis
13258_2019_862_MOESM2_ESM.xls (418 kb)
Supplementary material 2 (XLS 418 kb) Supplementary Table S2 The CgNF-YB9-corelated genes

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Copyright information

© The Genetics Society of Korea 2019

Authors and Affiliations

  1. 1.Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Life and PharmaceuticalHainan UniversityHaikouChina

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