Construction of genetic linkage map and identification of QTLs related to agronomic traits in DH population of maize (Zea mays L.) using SSR markers

  • Jae-Keun Choi
  • Kyu Jin Sa
  • Dae Hyun Park
  • Su Eun Lim
  • Si-Hwan Ryu
  • Jong Yeol Park
  • Ki Jin Park
  • Hae-Ik Rhee
  • Mijeong Lee
  • Ju Kyong LeeEmail author
Research Article



In this study, we used phenotypic and genetic analysis to investigate Double haploid (DH) lines derived from normal corn parents (HF1 and 11S6169). DH technology offers an array of advantages in maize genetics and breeding as follows: first, it significantly shortens the breeding cycle by development of completely homozygous lines in two or three generations; and second, it simplifies logistics, including requiring less time, labor, and financial resources for developing new DH lines compared with the conventional RIL population development process.


In our study, we constructed a maize genetic linkage map using SSR markers and a DH population derived from a cross of normal corn (HF1) and normal corn (11S6169).


The DH population used in this study was developed by the following methods: we crossed normal corn (HF1) and normal corn (11S6169), which are parent lines of a normal corn cultivar, in 2014; and the next year, the F1 hybrids were crossed with a tropicalized haploid inducer line (TAIL), which is homozygous for the dominant marker gene R1-nj (Nanda and Chase in Crop Sci 6:213–215, 1966), and we harvested seeds of the haploid lines.


A total of 200 SSR markers were assigned to 10 linkage groups that spanned 1145.4 cM with an average genetic distance between markers of 5.7 cM. 68 SSR markers showed Mendelian segregation ratios in the DH population at a 5% significance threshold. A total of 15 quantitative trait loci (QTLs) for plant height (PH), ear height (EH), ear height ratio (ER), leaf length (LL), ear length (EL), set ear length (SEL), set ear ratio (SER), ear width (EW), 100 kernel weight (100 KW), and cob color (CC) were found in the 121 lines in the DH population.


The results of this study may help to improve the detection and characterization of agronomic traits and provide great opportunities for maize breeders and researchers using a DH population in maize breeding programs.


Maize Genetic map DH population QTLs Agronomic trait SSR marker 



This study was supported by the Cooperative Research Program for Agriculture Science and Technology Development (Project title #PJ013157012018, Project #PJ013308012018), Rural Development Administration, Republic of Korea, and the Golden Seed Project (No. 213009-05-1-WT821, PJ012650012017), Ministry of Agriculture, Food, and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA), and Korea Forest Service (KFS), Republic of Korea.

Compliance with ethical standards

Conflict of interest

Jae-Keun Choi declares that he has no conflict of interest. Kyu Jin Sa declares that he has no conflict of interest. Dae Hyun Park declares that he has no conflict of interest. Su Eun Lim declares that she has no conflict of interest. Si-Hwan Ryu declares that he has no conflict of interest. Jong Yeol Park declares that he has no conflict of interest. Ki Jin Park declares that he has no conflict of interest. Hae-Ik Rhee declares that he has no conflict of interest. Mijeong Lee declares that she has no conflict of interest. Ju Kyong Lee declares that he has no conflict of interest.

Ethical approval

This article does not contain any studies with human subjects or animals performed by any of the above authors.


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© The Genetics Society of Korea 2019

Authors and Affiliations

  1. 1.Gangwon-do Agricultural Research and Extension ServicesMaize Research InstituteHongcheonKorea
  2. 2.Department of Applied Plant Sciences, College of Agriculture and Life SciencesKangwon National UniversityChuncheonKorea
  3. 3.Department of Medical BiotechnologyKangwon National UniversityChuncheonKorea
  4. 4.Department of Anatomy Cell BiologyKangwon National University School of MedicineChuncheonKorea

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