Genes & Genomics

, Volume 41, Issue 7, pp 839–847 | Cite as

Pre-labelled oligo probe-FISH karyotype analyses of four Araliaceae species using rDNA and telomeric repeat

  • Hui Chao Zhou
  • Remnyl Joyce Pellerin
  • Nomar Espinosa Waminal
  • Tae-Jin YangEmail author
  • Hyun Hee KimEmail author
Research Article



The family Araliaceae contains many medicinal species including ginseng of which the whole genome sequencing analyses have been going on these days.


To characterize the chromosomal distribution of 5S and 45S rDNAs and telomeric repeat in four ginseng related species of Aralia elata (Miq.) Seem., Dendropanax morbiferus H. Lév., Eleutherococcus sessiliflorus (Rupr. Et Maxim.) Seem., Kalopanax septemlobus (Thunb. ex A.Murr.) Koidz.


Pre-labelled oligoprobe (PLOP)-fluorescence in situ hybridization (FISH) was carried out.


The chromosome number of A. elata was 2n = 24, whereas that of the other three species of D. morbiferus, E. sessiliflorus, and K. septemlobus was 2n = 48, corresponding to diploid and tetraploid, respectively, based on the basic chromosome number x = 12 in Araliaceae. In all four species, one pair of 5S signals were detected in the proximal regions of the short arms of chromosome 3, whereas in K. septemlobus, the 5S rDNA signals localized in the subtelomeric region of short arm of chromosome 3, while all the 45S rDNA signals localized at the paracentromeric region of the short arm of chromosome 1. And the telomeric repeat signals were detected at the telomeric region of both short and long arms of most chromosomes.


The PLOP-FISH was very effective compared with conventional FISH method. These results provide useful comparative cytogenetic information to better understand the genome structure of each species and will be useful to trace the history of ginseng genomic constitution.


PLOP-FISH 5S and 45S rDNA Telomeric repeat Karyotype Araliaceae 



This work was carried out with the support of the Cooperative Research Program for Agriculture Science & Technology Development (PJ01311902), Rural Development Administration, Republic of Korea.


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Copyright information

© The Genetics Society of Korea 2019

Authors and Affiliations

  1. 1.Department of Life Sciences, Chromosome Research InstituteSahmyook UniversitySeoulRepublic of Korea
  2. 2.Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, College of Agriculture and Life SciencesSeoul National UniversitySeoulRepublic of Korea

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