Comparative transcriptome analysis in the hepatopancreas of Helice tientsinensis exposed to the toxic metal cadmium
The mudflat crab Helice tientsinensis is one of the most commercially valuable species for crabmeat production due to its delicious taste. These crabs are mainly found in coastal wetland where they are seriously threatened by toxic heavy metal pollution. In crustaceans, the hepatopancreas is an important organ for detoxification, and metal toxic substances can be converted to non-toxic or less toxic compounds in this organ.
To develop a better understanding of the molecular response of H. tientsinensis to the toxic metal cadmium (Cd) and provide a molecular basis for the toxic metal tolerance of H. tientsinensis.
In this study, we performed comparative hepatopancreas transcriptome analysis between H. tientsinensis unexposed (as control) and exposed to the toxic metal Cd for 48 h.
We identified 1089 Cd stress significantly-upregulated and 1560 Cd stress significantly-downregulated unigenes. Functional categorization and annotation of these differentially-expressed genes (DEGs) demonstrated that the response to Cd stress in the hepatopancreas of H. tientsinensis mainly involves “antioxidant activity”, “detoxification”, “toxin degradation activity” and “immune system process”. In addition, five genes (ABCC1, NDUFAF5, ASTL, DES1, CYP27A) were identified as possible major targets for toxic metal tolerance.
This is the first time reporting that the response of H. tientsinensis to Cd exposure at the transcriptome level, and it lays the foundation for understanding the molecular mechanisms of the response of H. tientsinensis to environmental toxic metal stress.
KeywordsHelice tientsinensis Cadmium stress Transcriptome Detoxification Toxic metal tolerance
This study was funded by the National Natural Science Foundation of China (Grant Number 31702014), and Doctoral Scientific Research Foundation of Yancheng Teachers University to ZFW, and Open Foundation of Jiangsu Key Laboratory for Bioresources of Saline Soils (Grant Number JKLBS2016007), and Jiangsu Province Undergraduate Training Programs for Innovation and Entrepreneurship to RBL and XLS.
HYG, SLX, RBL, XLS and ZFW designed and conceived the experiment. BPT, ZFW, DT and LXS performed the data analysis and draft the manuscript. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare there are no competing interests.
The sampling location was not privately-owned or protected, and field sampling did not involve protected species.
- Almeida EAD, Bainy ACD, Loureiro APDM, Martinez GR, Miyamoto S, Onuki J, Barbosa FL, Garcia CCM, Prado FM, Ronsein GE (2007). Oxidative stress in Perna perna and other bivalves as indicators of environmental stress in the Brazilian marine environment: antioxidants, lipid peroxidation and DNA damage. Comp Biochem Physiol A Mol Integr Physiol 146:588–600CrossRefGoogle Scholar
- Bo Y, Lan W, Li Y, Na L, Qian W (2007) Effects of cadmium on hepatopancreatic antioxidant enzyme activity in freshwater crab Sinopotamon yangtsekiense. Acta Zoologica Sinica 1121–1128Google Scholar
- Dai A, Yang S (1991) Crabs of the China seas. Weather 5:335–338Google Scholar
- Gross PS, Bartlett TC, Browdy CL, Chapman RW, Warr GW (2001) Immune gene discovery by expressed sequence tag analysis of hemocytes and hepatopancreas in the Pacific White Shrimp, Litopenaeus vannamei, and the Atlantic White Shrimp. L. setiferus. Dev Comp Immunol 25:565–577CrossRefPubMedGoogle Scholar
- Kanehisa M, Goto S (2000) KEGG: Kyoto encyclopedia of genes and genomes. Nucl Aci Res 27:29–34Google Scholar
- Lehninger AL (1958) Oxidative phosphorylation. Expos Annu Biochim Med 11: 11–29Google Scholar
- Pan L, Ren J, Wu Z (2004) Effects of heavy metal ions on SOD, CAT activities of hepatopancreas and gill of the crab Eriocheir sinensis. J Ocean Univ China 34:189–194Google Scholar
- Poynton HC, Taylor NS, Hicks J, Colson K, Chan S, Clark C, Scanlan L, Loguinov AV, Vulpe C, Viant MR (2011) Metabolomics of microliter hemolymph samples enables an improved understanding of the combined metabolic and transcriptional responses of Daphnia magna to cadmium. Environ Sci Techno 45:3710–3717CrossRefGoogle Scholar
- Viguié F (1999) ABCC1 (ATP-binding cassette, sub-family C (CFTR/MRP), member 1). Atlas Genet Cytogenet Oncol Haematol 3:11–12Google Scholar
- Wang G (2007) P50-M high-throughput and automatic plasmid DNA preparation with SeqPrep technology. J Biom Tech 18:17Google Scholar
- Wang L, Sun H, Li C (2002) Effects of cadmium on spermatogenesis in freshwater crab (Sinopotamon yangtsekiense). Acta Zool Sin 48:677–684Google Scholar
- Westfall PH, Young SS (1989) p value adjustments for multiple tests in multivariate binomial models. J Am Stat Assoc 84:780–786Google Scholar
- Xu JM (2012) Population genetic structure and phylogeography of the mud-flat crabs Helice tientsinensis and Helice latimera along the coast of China seas based on mitochondrial DNA. Afr J Biotechnol 11:3738–3750Google Scholar