Effect of 1-aminocyclopropane-1-carboxylic acid (ACC)-induced ethylene on cellulose synthase A (CesA) genes in flax (Linum usitatissimum L. ‘Nike’) seedlings
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Cellulose microfibril is a major cell wall polymer that plays an important role in the growth and development of plants. The gene cellulose synthase A (CesA), encoding cellulose synthases, is involved in the synthesis of cellulose microfibrils. However, the regulatory mechanism of CesA gene expression is not well understood, especially during the early developmental stages.
To identify factor(s) that regulate the expression of CesA genes and ultimately control seedling growth and development.
The presence of cis-elements in the promoter region of the eight CesA genes identified in flax (Linum usitatissimum L. ‘Nike’) seedlings was verified, and three kinds of ethylene-responsive cis-elements were identified in the promoters. Therefore, the effect of ethylene on the expression of four selected CesA genes classified into Clades 1 and 6 after treatment with 10−4 and 10−3 M 1-aminocyclopropane-1-carboxylic acid (ACC) was examined in the hypocotyl of 4–6-day-old flax seedlings.
ACC-induced ethylene either up- or down-regulated the expression of the CesA genes depending on the clade to which these genes belonged, age of seedlings, part of the hypocotyl, and concentration of ACC.
Ethylene might be one of the factors regulating the expression of CesA genes in flax seedlings.
KeywordsCesA Gene expression Ethylene 1-Aminocyclopropane-1-carboxylic acid Flax Seedlings
We thank Professor Dr. Grzegorz Spychalski from the Institute of Natural Fibres and Medicinal Plants for providing the seeds of flax.
- Paek SH, Oh SE (2012) Cloning and characterization of cellulose synthase genes involved in primary cell wall synthesis in flax (Linum usitatissimum L.) In: Book of Abstracts for 10th International Congress on Plant Molecular Biology, Jeju, Korea, pp. 268Google Scholar
- Petti C, Hirano K, Stork J, DeBolt S (2015) Mapping of cellulose-deficient mutant named dwarf1-1 in Sorghum bicolor to the green revolution gene gibberellin20-oxidase reveals a positive regulatory association between gibberellin and cellulose biosynthesis. Plant Physiol 169:705–716CrossRefPubMedPubMedCentralGoogle Scholar
- Vandenbussche F, Petrášek J, Žádníková P, Hoyerová K, Pešek B, Raz V, Swarup R, Bennett M, Zažímalová E, Benková E, Van Der Straeten D (2010) The auxin influx carriers AUX1 and LAX3 are involved in auxin-ethylene interactions during apical hook development in Arabidopsis thaliana seedlings. Development 137:597–606CrossRefPubMedGoogle Scholar