LncRNA Gm2044 highly expresses in spermatocyte and inhibits Utf1 translation by interacting with Utf1 mRNA
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Spermatogenesis is a process which includes the following phases: spermatogonial stem cell proliferation and differentiation, spermatogonia, spermatocyte, spermatid and mature sperm. Spermatogenic failure is the important factor resulting in male infertility. Recent studies showed that long noncoding RNA (lncRNA) have been found to be involved in the regulation of male reproduction. However, lncRNA associated with spermatogenesis and their mechanisms of action are unclear. The aim of this study is to explore the role and molecular mechanism of lncRNA in spermatogenesis. LncRNA microarray of germ cells and bioinformatic analysis showed lncRNA Gm2044 may play potential roles in spermatogenesis. The expression level of RNA and protein were analyzed by RT-qPCR and western blotting, respectively. The interaction of lncRNA with mRNA was detected by RNA pull down and cellular proliferation was measured using CCK-8 reagent. Testis-enriched lncRNA Gm2044 is abundant in mouse spermatocytes. Gm2044 can suppress the translation of adjacent spermatogenesis-related gene Utf1 by interacting with Utf1 mRNA. Furthermore, the proliferation of mouse spermatogonia GC-1 cell line and spermatocyte GC-2 cell line was inhibited by Gm2044. CONCLUSION: LncRNA Gm2044 was identified to inhibit Utf1 mRNA translation and play important roles in spermatogenesis.
KeywordsLncRNA Gm2044 Spermatocyte UTF1 GC-1 GC-2
This work was supported by Natural Science Foundation of Anhui Province (China) (1608085QH199), Natural Science Foundation of Bengbu Medical College (China) (BYKY1406ZD), Undergraduate Training Program for Innovation and Entrepreneurship of Anhui Province (China) (201610367003) and Research Foundation for Excellent Youth Scholars of Anhui Provincial Department of Education (China) (2012SQRL092). We thank Dr. Fei Sun (Shanghai Jiaotong University, China) for offering GC-1 and GC-2 cell lines.
KH and ML conceived and performed this research; ML wrote this manuscript, LL and YL analyzed results and reviewed this manuscript.
Compliance with ethical standards
Conflict of interest
Ke Hu declares that she has no conflict of interest. Leina Li declares that she has no conflict of interest. Yaping Liao declares that he has no conflict of interest. Meng Liang declares that he has no conflict of interest.
This study received ethical approval from the institutional review boards of the Bengbu medical college.
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