Expression analysis of miR-221-3p and its target genes in horses
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A microRNA (miRNA) is a small non-coding RNA (ncRNA) approximately 20 nucleotides long and it affects gene expression through mRNA cleavage or translational repression. Horses (Equus caballus) have been domesticated and bred to enhance their speed for racing. It has been studied extensively with genetic diversity, origins and evolution.
We examined expression patterns of miR-221-3p and its target gene CDKN1C in various horse tissues.
We used bioinformatic tools to examine target gene, seed region and evolutionary conservation of miR-221-3p. The expression patterns of miR-221-3p and its target gene CDKN1C were analyzed by quantitative polymerase chain reaction (qPCR).
Among eight tissues of horse, miR-221-3p was highly expressed in cerebellum and spleen. On the other hand, only medulla was highly expressed in CDKN1C gene.
Our study provides expression data of miR-221-3p and CDKN1C gene in horse and suggests the fundamental information for future studies in relation to functional importance.
KeywordsEquus caballus Micro RNA 221-3p CDKN1C Quantitative polymerase chain reaction (qPCR) Expression analysis
This work was supported by a grant from the Ministry of Education, Republic of Korea (NRF-2015R1D1A1A01056650).
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflicts of interest.
The animal protocol and sample extraction method in this study were reviewed by the Pusan National University-Institutional Animal Care and Use Committee (PNU-IACUC) for the ethical procedures and scientific care (approval number PNU-2013-0411).
Research involving human participants
This article does not contain any studies with human participants performed by any of the authors.
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