Cytotoxicity and genotoxicity of silver nanoparticles in Chinese Hamster ovary cell line (CHO-K1) cells
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Biomedical and pharmaceutical products comprising silver nanoparticles are attracting interest due to their potent antibacterial activities. For their safe use it has become imperative to test their cyto-genotoxic potential. In the present study the cytotoxicity and genotoxicity of three different sizes of AgNPs ranging from 15 to 22 nm and at concentrations 0.005–500 μg/ml were studied in Chinese Hamster ovary cell line (CHO-K1) cells. Cytoxicity was assessed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and for genotoxicity comet, and micronucleus assays were utilized. AgNPs were able to internalize CHO-K1 cells and cause cytotoxicity at concentrations 0.005–500 μg/ml. AgNP‐induced cyto-genotoxicity in CHO-K1 cells could be attributed to its smaller primary size. AgNP-C of size ~ 15 nm was the most potent among the three AgNPs. The genotoxic response was biphasic that increased at lower concentrations (0.005–0.025 μg/ml) and decreased at higher concentrations (0.05–0.1 μg/ml) after 24 h of exposure. Such potential in vitro genotoxic effect of AgNPs remains to be further confirmed in animal cells in vivo.
KeywordsSilver nano particles DNA damage Micronuclei Cytotoxicity Cellular uptake
Authors are thankful from Iran Nano Initiative Council (INIC) (Grant No. 1188) for financial support of present study.
- 8.Gorth DJ, Rand DM, Webster TJ. Silver nanoparticle toxicity in Drosophila: size does matter. Int J Nanomed. 2011;6:343–50.Google Scholar
- 10.Heshmati M, Arbabi Bidgoli S, Khoei S, Rezayat SM, Parivar K. Mutagenic effects of nanosilver consumer products: a new approach to physicochemical properties. Iran J Pharm Res. 2015;14:1171–80.Google Scholar
- 14.Lee HJ, Yeo SY, Jeong SH. Antibacterial effect of nanosized silver colloidal solution on textile fabrics. J Mater Sci. 2003;38:199–204.Google Scholar
- 23.Raesian A, Arbabi Bidgoli S, Sorkhabadi R, Mahdi S. Dermal toxicity of colloidal nanosilver in albino rabbit: a new approach to physicochemical properties. Nanomed Res J. 2017;2:142–9.Google Scholar