DNA damage in the peripheral blood lymphocytes of asthmatic patients in relation to disease progression
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With increased incidences in recent times especially in the urban population, asthma represents a major global concern. The inflammatory reaction of the airways in asthmatic patients leads to generation of reactive oxygen intermediates which interfere with the basic structure of DNA causing genomic instability. The present study was aimed to assess the DNA damage in the peripheral blood lymphocytes in asthmatic subjects. Blood samples from 95 asthmatic subjects and 57 controls were analysed for DNA damage using Comet assay. Statistical analysis was done using student’s t test and ANOVA. p < 0.05 was considered significant. Significant elevation was observed in % tail DNA (p < 0.05) in asthmatic subjects as compared to their non-asthmatic counterparts. Tail length, tail and olive moment were also markedly higher in asthmatics subjects (p < 0.05). The DNA damage was evidently higher in patients with severe asthma as compared to those with mild and moderate asthma (p < 0.05). The genomic instability was positively correlated with the age of subjects and severity of asthma which implied association of DNA damage with asthma and its progression with duration and severity.
KeywordsAsthma Comet assay Oxidative stress DNA damage
Financial assistance received from Haryana State Council for Science and Technology, Panchkula, in the form of JRF to Manisha Saini is gratefully acknowledged. We are grateful to Dr. Madan Khanna and Dr. Subhash Garg for providing blood samples, and to the authorities of Kurukshetra University for providing Laboratory and other necessary facilities.
Compliance with ethical standards
Conflict of interest
Authors declare that they have no conflict of interest.
All procedures performed in the present study were in accordance with the ethical standards of the Institutional Ethics Committee, KUK and with the 1964 Helsinki declaration and its later amendments (2013 Helsinki declaration). An appropriate written informed consent was taken from each subject prior taking samples and ethical approval was obtained from Institutional Ethics Committee of Kurukshetra University, Kurukshetra.
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