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Smac Gene Delivery by the Glycol Chitosan with Low Molecular Weight Polyethylenimine Induces Apoptosis of Cancer Cells for Combination Therapy with Etoposide

  • Yoonhee Bae
  • Young Hwa Lee
  • Kyung Soo Ko
  • Jin HanEmail author
  • Joon Sig ChoiEmail author
Article
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Abstract

We developed polymer-based nanoparticle carriers for gene delivery, glycol chitosan (GC)-methyl acrylate-low molecular weight polyethylenimine (800Da PEI), GMP, and investigated their ability to deliver second mitochondria-activation of caspase (Smac) gene into cancer cell lines. We analyzed cytotoxicity level and gene transfection efficiency in GBL-14, U373-MG, MDA-MB-231, and dermal fibroblasts (HDF) cell lines. GMP exhibited significantly lower cytotoxicity level, as compared with PEI25KD. Also, it showed high transfection efficiency in these cell lines. We treated these cells with a complex of GMP and Smac, and investigated their facilitated cellular uptake by flow cytometry. Also, analyses using cell cycle distribution, Annexin V staining, JC-1, and caspase-3 activity, indicated that apoptosis was induced by GMP/FLAG-Smac combined with etoposide in MDA-MB-231 cell line. This study demonstrates that combining the GMP/FLAG-Smac complex with chemotherapeutic agents could potentially be an effective approach for cancer therapy.

Keywords

Smac nanoparticle GMP apoptosis etoposide 

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Copyright information

© The Polymer Society of Korea and Springer 2019

Authors and Affiliations

  1. 1.Department of Biochemistry, College of Natural ScienceChungnam National UniversityDaejeonKorea
  2. 2.Department of Physiology, College of Medicine, Cardiovascular and Metabolic Disease CenterInje UniversityBusanKorea
  3. 3.Department of internal Medicine, Sanggye Paik Hospital, Cardiovascular and Metabolic Disease CenterInje UniversitySeoulKorea

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