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Chinese white truffles shape the ectomycorrhizal microbial communities of Corylus avellana

  • Mei Yang
  • Jie Zou
  • Chengyi Liu
  • Yujun Xiao
  • Xiaoping Zhang
  • Lijuan Yan
  • Lei Ye
  • Ping Tang
  • Xiaolin LiEmail author
Original Article

Abstract

Here, we investigated the influence of Chinese white truffle (Tuber panzhihuanense) symbioses on the microbial communities associated with Corylus avellana during the early development stage of symbiosis. The microbial communities associated with ectomycorrhizae, and associated with roots without T. panzhihuanense colonization, were determined via high-throughput sequencing of bacterial 16S rRNA genes and fungal ITS genes. Microbial community diversity was higher in the communities associated with the ectomycorrhizae than in the control treatment. Further, bacterial and fungal community structures were different in samples containing T. panzhihuanense in association with C. avellana compared to the control samples. In particular, the bacterial genera Rhizobium, Pedomicrobium, and Herbiconiux were more abundant in the ectomycorrhizae, in addition to the fungal genus Monographella. Moreover, there were clear differences in some physicochemical properties among the rhizosphere soils of the two treatments. Statistical analyses indicated that soil properties including exchangeable magnesium and exchangeable calcium prominently influenced microbial community structure. Lastly, inference of bacterial metabolic functions indicated that sugar and protein metabolism functions were significantly more enriched in the communities associated with the ectomycorrhizae from C. avellana mycorrhized with T. panzhihuanense compared to communities from roots of cultivated C. avellana without T. panzhihuanense. Taken together, these results highlight the interactions among ectomycorrhizal fungi, soil properties, and microbial communities that are associated with host plants and further our understanding of the ecology and cultivation of the economically important T. panzhihuanense truffles.

Keywords

Tuber panzhihuanense Corylus avellana High-throughput sequencing Microbial communities Soil properties 

Notes

Acknowledgments

We would like to thank LetPub (www.letpub.com) for providing linguistic assistance during the preparation of this manuscript.

Funding

This work was supported by the Science and Technology Support Project in Sichuan Province (2016NYZ0040) and the Sichuan Mushroom Innovation Team.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

This research does not involve human participants and/or animals.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Università degli studi di Milano 2019

Authors and Affiliations

  1. 1.Panzhihua Academy of Agricultural and Forestry SciencesPanzhihuaChina
  2. 2.Soil and Fertilizer InstituteSichuan Academy of Agricultural SciencesChengduChina
  3. 3.Department of Microbiology, College of ResourcesSichuan Agricultural UniversityChengduChina
  4. 4.Aquatic Geomicrobiology, Institute of BiodiversityFriedrich Schiller University JenaJenaGermany

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