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Antibacterial activity of endophytic fungi isolated from Sceletium tortuosum L. (Kougoed)

  • Madira Coutlyne ManganyiEmail author
  • Thierry Regnier
  • Christ-Donald Kaptchouang Tchatchouang
  • Cornelius Carlos Bezuidenhout
  • Collins Njie Ateba
Short Communication

Abstract

Endophytic fungi have the ability to co-exist with their host plants without causing any harm and are beneficial to both the plant and the fungi. The current study determined the antimicrobial properties and identify the chemical compounds of secondary metabolites produced by endophytic fungi isolated from Sceletium tortuosum L. A total of 60 endophytic fungi produced secondary metabolites that were detected after fermentation and extraction. Antibacterial properties of the secondary metabolites were determined using the disc diffusion assay against pathogenic environmental Gram-positive and Gram-negative bacteria as well as control stains. The chemical compounds were characterized by GC-MS. Overall, 15% of fungal extracts displayed narrow spectrum of activity against the bacteria strains. Despite this, none of the fungal extracts inhibited growth of Enterococcus faecalis (ATCC S1299) and Enterococcus gallinarum (ATCC 700425) while Bacillus cereus (ATCC 10876) was the most susceptible against the fungal extracts. Fusarium oxysporum (GG 008) with accession no. KJ774041.1 displayed significant antibacterial activity that was linked to high levels of 5-hydroxymethylfurfural (HMF) and octadecanoic acid as revealed by GC-MS. This study revealed the presence of bioactive secondary metabolites with antibacterial activities from fungi isolated from Sceletium tortuosum L.

Keywords

Endophytic fungi Sceletium tortuosum Secondary metabolites Bioactive compounds GC-MS analysis Fusarium oxysporum 

Notes

Funding

We would like to express our sincere gratitude to North-West University Postgraduate Merit Bursary and the National Research Foundation (Grant UID No: 105510) for the financial support.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

Not applicable (N/A).

Informed consent

Not applicable (N/A). This research does not involve human participants and/or animals; therefore, no informed consent is needed.

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Copyright information

© Università degli studi di Milano 2019

Authors and Affiliations

  • Madira Coutlyne Manganyi
    • 1
    Email author
  • Thierry Regnier
    • 2
  • Christ-Donald Kaptchouang Tchatchouang
    • 1
  • Cornelius Carlos Bezuidenhout
    • 3
  • Collins Njie Ateba
    • 1
    • 4
  1. 1.Department of MicrobiologyNorth-West University—Mafikeng CampusMmabathoSouth Africa
  2. 2.Department of Biotechnology and Food TechnologyTshwane University of TechnologyPretoriaSouth Africa
  3. 3.Unit for Environmental Sciences and ManagementNorth-West University—Potchefstroom CampusPotchefstroomSouth Africa
  4. 4.Food Security and Safety Niche Area, Faculty of Agriculture, Science and TechnologyNorth-West University—Mafikeng CampusMmabathoSouth Africa

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