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First outbreak reported caused by Erysipelothrix species strain 2 in turkeys from poultry-producing farms in Brazil

  • Patrícia G. Hoepers
  • Thais F. M. dos Reis
  • Eliane P. Mendonça
  • Daise A. Rossi
  • Priscilla K. Koerich
  • Tiago V. J. França
  • João P. Zuffo
  • Edson C. V. Junior
  • Belchiolina B. FonsecaEmail author
Short Communication
  • 3 Downloads

Abstract

Purpose

The aims of this study were to report the first isolation of Erysipelothrix sp. strain 2 (ES2) associated with clinical signs of diseases as well as mortality in turkeys and identify the antimicrobial resistance of the isolates.

Methods

We evaluated 118 farms for bacteriological analysis and TaqMan real-time PCR to identify the microorganism in different organs. After this, we made the epidemiological analysis between the positive flocks and the mortality mean. We performed the sequencing of the 16S rRNA region and the assessment of antimicrobial resistance.

Results

We have identified 18 (15.25%) as ES2-positive flocks, without any other species from the same genus being found. After analysing the organ samples, we found liver as the organ of choice for the isolation of the ES2. The sequencing of 16S rRNA region of ES2 identified high homology with E. tonsillarum and E. rhusiopathiae, suggesting that it is not the best-suited target to identify this species. We have found a positive association between isolation of the bacteria in organs and flocks’ mortality. Positive flocks had a mortality mean rate of 6.87%, which is significantly greater than 3.76% in negative flocks. Ill turkeys had gross lesions of generalized septicaemia. The bacterial isolates showed high resistance to fosfomycin and trimethoprim/sulfamethoxazole and sensibility to norfloxacin, amoxicillin and lincomycin/spectinomycin.

Conclusion

This is the first study in the world that addressed ES2 as the causative agent of erysipelas in turkey.

Keywords

Poultry Erysipelas Septicaemia 16S rRNA region sequencing Antimicrobial resistance 

Notes

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for their financial support.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflict of interests.

Research involving human animals

The necropsied birds of this work were naturally and recently dead, that by Brazilian Law No. 11,794 of October 8, 2008, there is no need for an evaluation by the Ethics Committee in animal research (because it deals with the handling of dead animals). Even so, we have communicated the Committee on Ethics in the Use of Animals of the Federal University of Uberlandia that certified our work with the number A004/19.

Supplementary material

13213_2019_1505_MOESM1_ESM.pdf (410 kb)
ESM 1 (PDF 410 kb)

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Copyright information

© Università degli studi di Milano 2019

Authors and Affiliations

  • Patrícia G. Hoepers
    • 1
  • Thais F. M. dos Reis
    • 1
  • Eliane P. Mendonça
    • 2
  • Daise A. Rossi
    • 1
  • Priscilla K. Koerich
    • 3
  • Tiago V. J. França
    • 4
  • João P. Zuffo
    • 5
  • Edson C. V. Junior
    • 1
  • Belchiolina B. Fonseca
    • 1
    Email author
  1. 1.Universidade Federal de UberlândiaUberlândiaBrazil
  2. 2.Universidade Federal de UberabaUberlândiaBrazil
  3. 3.Universidade Federal do Rio Grande do SulPorto AlegreBrazil
  4. 4.BRF Brasil FoodSão PauloBrazil
  5. 5.UDESC-Universidade do estado de Santa CatarinaCedima, Centro de Diagnóstico de Microbiologia AnimalFlorianopolisBrazil

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