Thaumarchaeota affiliated with Soil Crenarchaeotic Group are prevalent in the alkaline soil of an alpine grassland in northwestern China
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Thaumarchaeota are key players within the global nitrogen cycle. Investigations of the Thaumarchaeota communities are important for an integrated understanding of nitrogen nutrient cycle in soil ecosystems. Therefore, the objective of this study was to examine the presence and diversity of Thaumarchaeota within an alkaline soil in the Bayinbuluke alpine grassland, China.
The community DNAs were directly extracted from soil samples, collected on 15 July 2014, and paired-end V5–V6 amplicons of the 16S rRNA gene were sequenced by Illumina Miseq. Sequencing reads were processed using the Quantitative Insights Into Microbial Ecology (QIIME) v. 1.8.0 pipeline. After quality control, the validated sequence reads were classified into different operational taxonomic units (OTUs) based on a 97% identity level, using the Uclust algorithm to generate stable OTUs. The longest sequence in each cluster was chosen to be the representative sequence, and sequences were annotated using the Silva rRNA database project.
In the analyzed grassland soil, Thaumarchaeota had a relative abundance of 3.65 to 51.07% of the microbial community (mean = 20.20%), representing the most dominant phylum. The thaumarchaeal community was dominated by the Soil Crenarchaeotic Group (SCG, 34.55 to 99.82%, mean = 95.10%), with specifically low fraction of the ammonia-oxidizing genus Candidatus Nitrososphaera (2.83 to 30.37%, mean = 13.10%) and remaining unclassified genus.
Our results show Thaumarchaeota affiliated with SCG were prevalent in the alkaline soil of this grassland.
KeywordsAlkaline soil, Illumina amplicon sequencing, Thaumarchaeota Soil Crenarchaeotic Group Candidatus Nitrososphaera
We thank staff at the Institute of Lake Bosten, of the Environmental Protection Bureau of Bayingolin Mongolia Autonomous Prefecture, for help with sample collection. We are grateful to the editor and anonymous reviewers for their constructive comments and helpful suggestions.
This study was funded by the “One-Three-Five” Strategic Planning of Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences (Grant No. NIGLAS2017GH05), the National Natural Science Foundation of China (Grant No. 41790423) and the Special Environmental Research Funds for Public Welfare of the State Environmental Protection Administration (Grant No. 201309041).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Research involving human participants and/or animals
This article does not contain any studies with human participants or animals.
A statement regarding informed consent is not applicable for this study.
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