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Annals of Microbiology

, Volume 69, Issue 8, pp 867–870 | Cite as

Thaumarchaeota affiliated with Soil Crenarchaeotic Group are prevalent in the alkaline soil of an alpine grassland in northwestern China

  • Keqiang ShaoEmail author
  • Xingyu Jiang
  • Yang Hu
  • Xiangming Tang
  • Guang Gao
Short Communication
  • 17 Downloads

Abstract

Purpose

Thaumarchaeota are key players within the global nitrogen cycle. Investigations of the Thaumarchaeota communities are important for an integrated understanding of nitrogen nutrient cycle in soil ecosystems. Therefore, the objective of this study was to examine the presence and diversity of Thaumarchaeota within an alkaline soil in the Bayinbuluke alpine grassland, China.

Methods

The community DNAs were directly extracted from soil samples, collected on 15 July 2014, and paired-end V5–V6 amplicons of the 16S rRNA gene were sequenced by Illumina Miseq. Sequencing reads were processed using the Quantitative Insights Into Microbial Ecology (QIIME) v. 1.8.0 pipeline. After quality control, the validated sequence reads were classified into different operational taxonomic units (OTUs) based on a 97% identity level, using the Uclust algorithm to generate stable OTUs. The longest sequence in each cluster was chosen to be the representative sequence, and sequences were annotated using the Silva rRNA database project.

Result

In the analyzed grassland soil, Thaumarchaeota had a relative abundance of 3.65 to 51.07% of the microbial community (mean = 20.20%), representing the most dominant phylum. The thaumarchaeal community was dominated by the Soil Crenarchaeotic Group (SCG, 34.55 to 99.82%, mean = 95.10%), with specifically low fraction of the ammonia-oxidizing genus Candidatus Nitrososphaera (2.83 to 30.37%, mean = 13.10%) and remaining unclassified genus.

Conclusion

Our results show Thaumarchaeota affiliated with SCG were prevalent in the alkaline soil of this grassland.

Keywords

Alkaline soil, Illumina amplicon sequencing, Thaumarchaeota Soil Crenarchaeotic Group Candidatus Nitrososphaera 

Notes

Acknowledgments

We thank staff at the Institute of Lake Bosten, of the Environmental Protection Bureau of Bayingolin Mongolia Autonomous Prefecture, for help with sample collection. We are grateful to the editor and anonymous reviewers for their constructive comments and helpful suggestions.

Funding

This study was funded by the “One-Three-Five” Strategic Planning of Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences (Grant No. NIGLAS2017GH05), the National Natural Science Foundation of China (Grant No. 41790423) and the Special Environmental Research Funds for Public Welfare of the State Environmental Protection Administration (Grant No. 201309041).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

This article does not contain any studies with human participants or animals.

Informed consent

A statement regarding informed consent is not applicable for this study.

Supplementary material

13213_2019_1492_MOESM1_ESM.docx (111 kb)
ESM 1 (DOCX 111 kb)

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Copyright information

© Università degli studi di Milano 2019

Authors and Affiliations

  • Keqiang Shao
    • 1
    Email author
  • Xingyu Jiang
    • 1
  • Yang Hu
    • 1
  • Xiangming Tang
    • 1
  • Guang Gao
    • 1
  1. 1.Taihu Laboratory for Lake Ecosystem Research, State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and LimnologyChinese Academy of SciencesNanjingChina

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