Community structure and functional diversity of epiphytic bacteria and planktonic bacteria on submerged macrophytes in Caohai Lake, southwest of China
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Epiphytic bacteria on the surfaces of submerged macrophytes play an important role in lake biodiversity and ecological processes. However, compared with planktonic bacteria, there is poor understanding of the community structure and function of epiphytic bacteria.
Here, we used 16S rRNA gene high-throughput sequencing and functional prediction analysis to explore the structural and functional diversity of epiphytic bacteria and planktonic bacteria of a typical submerged macrophyte (Potamogeton lucens) in Caohai Lake.
The results showed that the species composition of epiphytic and planktonic bacteria was highly similar as 88.89% phyla, 77.21% genera and 65.78% OTUs were shared by the two kinds of samples. Proteobacteria and Bacteroidetes were dominant phyla shared by the two kinds of communities. However, there are also some special taxa. Furthermore, the epiphytic bacterial communities exhibited significantly different structures from those in water, and the abundant OTUs had opposite constituents. The explained proportion of the planktonic bacterial community by aquatic environmental parameters is significantly higher than that of epiphytic bacteria, implying that the habitat microenvironment of epiphytic biofilms may be a strong driving force of the epiphytic bacterial community. Functional predictive analysis (Functional Annotation of Prokaryotic Taxa, FAPROTAX) found that epiphytic bacteria and planktonic bacteria are dominated by heterotrophic functions, but epiphytic bacteria have more prominent fermentation and denitrification functions (nitrate reduction, nitrate respiration, and nitrite respiration) than planktonic bacteria.
This study has increased our understanding of the communities and functions of epiphytic bacteria on submerged macrophyte leaves, and their role in lake denitrification cannot be ignored.
KeywordsEpiphytic bacteria Planktonic bacteria Diversity pattern Functional traits
This project was financially supported by the National Natural Science Foundation of China (41867056), Major Project of Guizhou Province (20163022), Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province (U1812401), and Guizhou Science and Technology Plan Project (20185769).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies performed by any of the authors with human participants.
Informed consent was obtained from all individual participants included in the study.
- Galand PE, Pereira O, Hochart C, Auguet JC, Debroas D (2018) A strong link between marine microbial community composition and function challenges the idea of functional redundancy. ISME J 12:2470–2478. https://doi.org/10.1038/s41396-018-0158-1
- Hassett DJ, Cuppoletti J, Trapnell B, Lymar SV, Rowe JJ, Sun Yoon S, Hilliard GM, Parvatiyar K, Kamani MC, Wozniak DJ, Hwang S-H, McDermott TR, Ochsner UA (2002) Anaerobic metabolism and quorum sensing by Pseudomonas aeruginosa biofilms in chronically infected cystic fibrosis airways: rethinking antibiotic treatment strategies and drug targets. Adv Drug Deliv Rev 54(11):1425–1443. https://doi.org/10.1016/S0169-409X(02)00152-7 Google Scholar
- Koo H, Strope BM, Kim EH, Shabani AM, Kumar R, Crowley MR, Andersen DT, Bej AK (2016) Draft genome sequence of Janthinobacterium sp. Ant5-2-1, isolated from proglacial Lake Podprudnoye in the Schirmacher oasis of East Antarctica. Genome Announcements 4(1):e01600–e01615. https://doi.org/10.1128/genomeA.01600-15 Google Scholar
- Langille MG, Zaneveld J, Caporaso JG, McDonald D, Knights D, Reyes JA, Clemente JC, Burkepile DE, Vega Thurber RL, Knight R, Beiko RG, Huttenhower C (2013) Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat Biotechnol 31(9):814–821. https://doi.org/10.1038/nbt.2676 Google Scholar
- Ma Q, Qu YY, Shen WL, Zhang ZJ, Wang JW, Liu ZY, Li DX, Li HJ, Zhou JT (2015) Bacterial community compositions of coking wastewater treatment plants in steel industry revealed by Illumina high-throughput sequencing. Bioresour Technol 179:436. https://doi.org/10.1016/j.biortech.2014.12.041 Google Scholar
- Matu A, Lum Nde A, Oosthuizen L, Hitzeroth A, Badenhorst M, Duba L, Gidaga M, Klinck J, Kriek I-M, Lekoma PJ, Nel L, dos Ramos SM, Rossouw J, Salomane N, Segone N, Serobe S, Tiyani T, Hugo CJ, Newman JD (2019) Draft genome sequences of seven Chryseobacterium type strains. Microbiology Resource Announcements 8(1):e01518–e01518. https://doi.org/10.1128/mra.01518-18 Google Scholar
- Madigan MT, Martinko JM, Stahl DA, Clark DP (2005) Brock biology of microorganisms (11th ed.) Benjamin Cummings: BostonGoogle Scholar
- Mori H, Maruyama F, Kato H, Toyoda A, Dozono A, Ohtsubo Y, Nagata Y, Fujiyama A, Tsuda M, Kurokawa K (2013) Design and experimental application of a novel non-degenerate universal primer set that amplifies prokaryotic 16S rRNA genes with a low possibility to amplify eukaryotic rRNA genes. DNA Res 21(2):217–227. https://doi.org/10.1093/dnares/dst052 Google Scholar
- Ordoñez OF, Lanzarotti E, Kurth D, Gorriti MF, Revale S, Cortez N, Vazquez MP, Farías ME, Turjanski AG (2013) Draft genome sequence of the polyextremophilic Exiguobacterium sp. strain S17, isolated from hyperarsenic lakes in the Argentinian Puna. Genome announcements 1(4):e00480–e00413. https://doi.org/10.1128/genomeA.00480-13 Google Scholar
- Patel V, Munot H, Shouche YS, Madamwar D (2014) Response of bacterial community structure to seasonal fluctuation and anthropogenic pollution on coastal water of Alang-Sosiya ship breaking yard, Bhavnagar, India. Bioresour Technol 161:362–370. https://doi.org/10.1016/j.biortech.2014.03.033 Google Scholar
- Singh S, James A, Bharose R (2017) Biological assessment of water pollution using periphyton productivity: a review. Nat Environ Pollut Technol 16(2):559–567Google Scholar
- Song YZ, Wang JQ, Gao YX, Xie XJ (2015) The physiological responses of Vallisneria natans to epiphytic algae with the increase of N and P concentrations in water bodies. Environ Sci Pollut Res 22(11):8480–8487. https://doi.org/10.1007/s11356-014-3998-x
- Su J, Kang D, Xiang W, Wu CX (2017) Periphyton biofilm development and its role in nutrient cycling in paddy microcosms. J Soils Sediments:810–819. https://doi.org/10.1007/s11368-016-1575-2
- Wei Z, Liu Y, Feng K, Li S, Wang S, Jin D, Zhang Y, Chen H, Yin H, Xu M, Deng Y (2018) The divergence between fungal and bacterial communities in seasonal and spatial variations of wastewater treatment plants. Sci Total Environ 628-629(1):969–978. https://doi.org/10.1016/j.scitotenv.2018.02.003 Google Scholar
- Wilhelm L, Singer GA, Fasching C, Battin TJ, Besemer K (2013) Microbial biodiversity in glacier-fed streams. ISME J 7(8):1651–1660. https://doi.org/10.1038/ismej.2013.44