Advertisement

Annals of Microbiology

, Volume 69, Issue 8, pp 819–828 | Cite as

Identification and characterization of ectoine-producing bacteria isolated from Can Gio mangrove soil in Vietnam

  • Doan Van ThuocEmail author
  • Tran Thi Hien
  • Kumar Sudesh
Original Article
  • 57 Downloads

Abstract

Purpose

The aim of this study was to characterize high ectoine-producing bacteria obtained from Can Gio mangrove soil samples in Vietnam.

Methods

Ectoine-producing bacteria were isolated from mangrove soil samples. The selected strains were identified using 16S rDNA sequence analysis, and their biochemical characteristics were also examined. The ability to produce ectoine at different NaCl concentrations and the effect of osmotic downshock solution on ectoine’s release rates and survival rates for the selected bacterial strains were investigated.

Results

Among more than 200 bacterial colonies isolated from soil samples, two strains exhibiting highest ectoine production (strains D227 and D228) were chosen for further studies. Both strains D227 and D228 were identified as Halomonas spp. and were closely related to Halomonas organivorans, sharing 99.4% 16S rDNA sequence similarity. At 6% (w/v) NaCl concentration, strains D227 and D228 presented the highest cell dry weight (CDW) of 3.85 and 3.55 g/l, respectively. At 18% NaCl concentration, maximum total ectoine (ectoine and hydroxyectoine) production of 16.4 and 18.1 wt% was achieved by strains D227 and D228, respectively. After 30 min of incubation in downshock solution containing 5% NaCl, high bacterial survival rates of 96% and 98%, and ectoines release rates of 61% and 76% were obtained by strains D227 and D228, respectively.

Conclusions

The accumulation and secretion of ectoine appear to be a typical adaptation strategy of some bacteria to survive under the changing saline conditions of mangrove ecosystem. To the best of our knowledge, this is the first report on ectoine production by halophilic bacteria isolated from mangrove soil. High ectoine-producing bacteria can be found in mangrove forest.

Keywords

Can Gio mangrove Compatible solutes Ectoine Halomonas Halophilic bacteria 

Notes

Funding

This research was supported by the Ministry of Education and Training (Grant B2007-SHP-32) and Vietnam National Foundation for Science and Technology Development (Nafosted) (Grant 106-NN.04-2016.11).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. Borges N, Ramos A, Raven NDH, Sharp RJ (2002) Comparative study of the thermostabilizing properties of mannosylglycerate and other compatible solutes on model enzymes. Extremophiles 6:209–216CrossRefGoogle Scholar
  2. Bünger J, Driller H (2004) Ectoine: an effective natural substance to prevent UVA-induced premature photoaging. Skin Pharmacol Physiol 17:232–237CrossRefGoogle Scholar
  3. Costa-Böddeker S, Thuyen LX, Schwarz A, Huy HD (2017) Diatom assemblages in surface sediments along nutrient and salinity gradients of Thi Vai estuary and Can Gio mangrove forest, Southern Vietnam. Estuar Coast 40:479–492CrossRefGoogle Scholar
  4. Detkova EN, Boltyanskaya YV (2007) Osmoadaptation of haloalkaliphilic bacteria: role of osmoregulators and their possible practical application. Microbiology 76:511–522CrossRefGoogle Scholar
  5. García MT, Mellado E, Ostos JC, Ventosa A (2004) Halomonas organivorans sp. nov., a moderate halophile able to degrade aromatic compounds. Int J Syst Evol Microbiol 54:1723–1728CrossRefGoogle Scholar
  6. Gasperotti AF, Revuelta MV, Studdert CA, Seitz MKH (2018) Identification of two different chemosensory pathways in representatives of the genus Halomonas. BMC Genomics 19:266CrossRefGoogle Scholar
  7. Guzmán H, Van-Thuoc D, Martín J, Hatti-Kaul R (2009) A process for the production of ectoine and poly(3-hydroxybutyrate) by Halomonas boliviensis. Appl Microbiol Biotechnol 84:1069–1077CrossRefGoogle Scholar
  8. Holguin G, Vazquez P, Bashan Y (2001) The role of sediment microorganisms in the productivity, conservation, and rehabilitation of mangrove ecosystems: an overview. Biol Fertil Soils 33:265–278CrossRefGoogle Scholar
  9. Kanapathipillai M, Lentzen G, Sierks M, Park CB (2004) Ectoine and hydroxyectoine inhibit aggregation and neurotoxicity of Alzheimer’s β-amyloid. FEBS Lett 579:4775–4780CrossRefGoogle Scholar
  10. Kunte HJ, Galinski EA, Trüper HG (1993) A modified FMOC-method for the detection of aminoacid type osmolytes and tetrahydropyrimidines (ectoines). J Microbiol Methods 17:129–136CrossRefGoogle Scholar
  11. Kunte HJ, Lentzen G, Galinski EA (2014) Industrial production of the cell protectant ectoine: production mechanisms, processes, and products. Curr Biotechnol 3:10–25CrossRefGoogle Scholar
  12. Lentzen G, Schwarz T (2006) Extremolytes: natural compounds from extremophiles for versatile applications. Appl Microbiol Biotechnol 72:623–634CrossRefGoogle Scholar
  13. Lippert G, Galinski EA (1992) Enzyme stabilization by ectoine-type compatible solutes: protection against heating, freezing and drying. Appl Microbiol Biotechnol 37:61–65CrossRefGoogle Scholar
  14. Malin G, Lapidot A (1996) Induction of synthesis of tetrahydropyrimidine derivatives in Streptomyces strains and their effect on Escherichia coli in response to osmotic and heat stress. J Bacteriol 178:385–395CrossRefGoogle Scholar
  15. Margesin R, Schinner F (2001) Potential of halotolerant and halophilic microorganisms for biotechnology. Extremophiles 5:73–83CrossRefGoogle Scholar
  16. Mata JA, Martínez-Cánovas J, Quesada E, Béjar V (2002) A detailed phenotypic characterization of the type strains of Halomonas species. Syst Appl Microbiol 25:360–375CrossRefGoogle Scholar
  17. Moh TH, Lau N-S, Furusawa G, Amirul A-AA (2017) Complete genome sequence of Microbulbifer sp. CCB-MM1, a halophile isolated from Matang mangrove forest, Malaysia. Stand Genomic Sci 12:36CrossRefGoogle Scholar
  18. Nagata S, Wang Y, Oshima A, Zhang L, Miyake H, Sasaki H, Ishida A (2008) Efficient cyclic system to yield ectoine using Brevibacterium sp. JCM 6894 subjected to osmotic downshock. Biotechnol Bioeng 99:941–948CrossRefGoogle Scholar
  19. Onraedt AE, Walcarius BA, Soetaert WK, Vandamme EJ (2005) Optimization of ectoine synthesis through fed-batch fermentation of Brevibacterium epidermis. Biotechnol Prog 21:1206–1212CrossRefGoogle Scholar
  20. Öztürk HU, Sariyar Akbulut B, Ayan B, Poli A (2015) Moderately halophilic bacterium Halomonas sp. AAD12: a promising candidate as a hydroxyectoine producer. J Microb Biochem Technol 7:262–268Google Scholar
  21. Pastor JM, Salvador M, Argandoña M, Bernal V (2010) Ectoines in cell stress protection: uses and biotechnological production. Biotechnol Adv 28:782–801CrossRefGoogle Scholar
  22. Roberts MF (2005) Organic compatible solutes of halotolerant and halophilic microorganism. Saline Syst (On-line Journal) 1:5CrossRefGoogle Scholar
  23. Sam K-K, Lau N-S, Furusawa G, Amirul A-AA (2018) Draft genome sequence of halophilic Hahella sp. strain CCB-MM4, isolated from Matang mangrove forests in Perak, Malaysia. Genome Announc 5:e01147–e01117Google Scholar
  24. Sauer T, Galinski EA (1998) Bacterial milking: a novel bioprocess for production of compatible solutes. Biotechnol Bioeng 57:306–313CrossRefGoogle Scholar
  25. Schröter MA, Meyer S, Hahn MB, Solomun T, Sturm H, Kunte HJ (2017) Ectoine protects DNA from damage by ionizing radiation. Sci Rep 7:15272CrossRefGoogle Scholar
  26. Ser H-L, Tan W-S, Mutalib N-SA, Yin W-F (2018) Genome sequence of Streptomyces mangrovisoli MUSC 149T isolated from intertidal sediments. Braz J Microbiol 49:13–15CrossRefGoogle Scholar
  27. Tamura K, Stecher G, Peterson D, Filipski A (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Bio Evol 30:2725–2729CrossRefGoogle Scholar
  28. Tuan VQ, Kuenzer C (2012) Can Gio mangrove biosphere reserve evaluation 2012: current status, dynamics and ecosystem services, IUCN, Hanoi, Vietnam 102 ppGoogle Scholar
  29. Van-Thuoc D, Guzmán H, Quillaguamán J, Hatti-Kaul R (2010) High productivity of ectoines by Halomonas boliviensis using a combined two-step fed-batch culture and milking process. J Biotechnol 147:46–51CrossRefGoogle Scholar
  30. Van-Thuoc D, Huu-Phong T, Thi-Binh N, Thi-Tho N, Minh-Lam D, Quillaguamán J (2012) Polyester production by halophilic and halotolerant bacterial strains obtained from mangrove soil samples located in northern Vietnam. MicrobiologyOpen 1:395–406CrossRefGoogle Scholar
  31. Van-Thuoc D, Hashim SO, Hatti-Kaul R, Mamo G (2013) Ectoine mediated protection of enzyme from the effect of pH and temperature stress: a study using Bacillus halodurans xylanase as a model. Appl Microbiol Biotechnol 97:6271–6278CrossRefGoogle Scholar
  32. Werkhäuser N, Bilstein A, Sonnemann U (2014) Treatment of allergic rhinitis with ectoine containing nasal spray and eye drops in comparison with azelastine containing nasal spray and eye drops or with cromoglycic acid containing nasal spray. J Allergy:176597Google Scholar
  33. Zhang LH, Lang YJ, Nagata S (2009) Efficient production of ectoine using ectoine-excreting strain. Extremophiles 13:717–724CrossRefGoogle Scholar

Copyright information

© Università degli studi di Milano 2019

Authors and Affiliations

  1. 1.Department of Biotechnology and Microbiology, Faculty of BiologyHanoi National University of EducationHanoiVietnam
  2. 2.School of Biological SciencesUniversiti Sains MalaysiaPenangMalaysia

Personalised recommendations