Advertisement

Annals of Microbiology

, Volume 69, Issue 4, pp 419–434 | Cite as

Demonstrating the potential of abiotic stress-tolerant Jeotgalicoccus huakuii NBRI 13E for plant growth promotion and salt stress amelioration

  • Sankalp Misra
  • Vijay Kant Dixit
  • Shashank Kumar Mishra
  • Puneet Singh ChauhanEmail author
Original Article
  • 155 Downloads

Abstract

The present study aimed to demonstrate the potential of abiotic stress-tolerant Jeotgalicoccus huakuii NBRI 13E for plant growth promotion and salt stress amelioration. NBRI 13E was characterized for abiotic stress tolerance and plant growth-promoting (PGP) attributes under normal and salt stress conditions. Phylogenetic comparison of NBRI 13E was carried out with known species of the same genera based on 16S rRNA gene. Plant growth promotion and rhizosphere colonization studies were determined under greenhouse conditions using maize, tomato, and okra. Field experiment was also performed to assess the ability of NBRI 13E inoculation for improving growth and yield of maize crop in alkaline soil. NBRI 13E demonstrated abiotic stress tolerance and different PGP attributes under in vitro conditions. Phylogenetic and differential physiological analysis revealed considerable differences in NBRI 13E as compared with the reported species for Jeotgalicoccus genus. NBRI 13E colonizes in the rhizosphere of the tested crops, enhances plant growth, and ameliorates salt stress in a greenhouse experiment. Modulation in defense enzymes, chlorophyll, proline, and soluble sugar content in NBRI 13E-inoculated plants leads to mitigate the deleterious effect of salt stress. Furthermore, field evaluation of NBRI 13E inoculation using maize was carried out with recommended 50 and 100% chemical fertilizer controls, which resulted in significant enhancement of all vegetative parameters and total yield as compared to respective controls. Jeotgalicoccus huakuii NBRI 13E is reported for the first time for its ability to develop a bioinoculant formulation for stress amelioration and improved crop productivity.

Keywords

Abiotic stress Defense enzymes Jeotgalicoccus huakuii Maize PGP attributes 

Notes

Acknowledgments

The authors acknowledge the Director, CSIR-National Botanical Research Institute for providing facilities and support during the study.

Funding

The authors acknowledge the financial assistance from the CSIR Network project MLP022 and In-house project OLP105.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

13213_2018_1428_MOESM1_ESM.docx (17 kb)
ESM 1 (DOCX 17 kb)

References

  1. Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126CrossRefGoogle Scholar
  2. Albaladejo I, Meco V, Plasencia F, Flores FB, Bolarin MC, Egea I (2017) Unravelling the strategies used by the wild tomato species Solanum pennellii to confront salt stress: from leaf anatomical adaptations to molecular responses. Environ Exper Bot 135:1–12CrossRefGoogle Scholar
  3. Alves M, Nogueira C, Ana AM, Chung AP, Morais PV, da Costa MS (2008) Nosocomiicoccus ampullae gen. nov., sp. nov., isolated from the surface of bottles of saline solution used in wound cleansing. Int J Syst Evol Microbiol 58:2939–2944CrossRefGoogle Scholar
  4. Ambardar S, Vakhlu J (2013) Plant growth promoting bacteria from Crocus sativus rhizosphere. World J Microbiol Biotechnol 29:2271–2279CrossRefGoogle Scholar
  5. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15CrossRefGoogle Scholar
  6. Ashraf M, Harris PJC (2013) Photosynthesis under stressful environments: an overview. Photosynthetica 51:163–190CrossRefGoogle Scholar
  7. AVRDC (2006) Vegetables matter. In: AVRDC – The World Vegetable Center. Shanhua, TaiwanGoogle Scholar
  8. Balsanelli E, de Baura VA, Pedrosa FD, de Souza EM, Monteiro RA (2014) Exopolysaccharide biosynthesis enables mature biofilm formation on abiotic surfaces by Herbaspirillum seropedicae. PLoS One 9:e110392CrossRefGoogle Scholar
  9. Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207CrossRefGoogle Scholar
  10. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287CrossRefGoogle Scholar
  11. Bharti N, Yadav D, Barnawal D, Maji D, Kalra A (2013) Exiguobacterium oxidotolerans, a halotolerant plant growth promoting rhizobacteria, improves yield and content of secondary metabolites in Bacopa monnieri (L.) Pennell under primary and secondary salt stress. World J Microbiol Biotechnol 29:379–387CrossRefGoogle Scholar
  12. Bistgani ZE, Siadat SA, Bakhshandeh A, Pirbalouti AG, Hashemi M (2017) Interactive effects of drought stress and chitosan application on physiological characteristics and essential oil yield of Thymus daenensis Celak. Crop J.  https://doi.org/10.1016/j.cj.2017.04.00
  13. Bric JM, Bostock RM, Silverstone SE (1991) Rapid in situ assay for indole acetic acid production by bacteria immobilized on a nitrocellulose membrane. Appl Environ Microbiol 57:535–538Google Scholar
  14. Bromham L, Saslis-Lagoudakis CH, Bennett TH, Flowers TJ (2013) Soil alkalinity and salt tolerance: adapting to multiple stresses. Biol Lett 9:20130642CrossRefGoogle Scholar
  15. Bui EN (2013) Soil salinity: a neglected factor in plant ecology and biogeography. J Arid Environ 92:14–25CrossRefGoogle Scholar
  16. Bui EN, Thornhill A, Miller JT (2014) Salt- and alkaline-tolerance are linked in Acacia. Biol Lett 10:20140278CrossRefGoogle Scholar
  17. Chakraborty U, Chakraborty BN, Chakraborty AP, Dey PL (2013) Water stress amelioration and plant growth promotion in wheat plants by osmotic stress tolerant bacteria. World J Microbiol Biotechnol 29:789–803CrossRefGoogle Scholar
  18. Chauhan PS, Nautiyal CS (2010) The purB gene controls rhizosphere colonization by Pantoea agglomerans. Lett Appl Microbiol 50:205–210CrossRefGoogle Scholar
  19. Chen S, Xing J, Lan H (2012) Comparative effects of neutral salt and alkaline salt stress on seed germination, early seedling growth and physiological response of a halophyte species Chenopodium glaucum. Afr J Biotechnol 11:9572–9581Google Scholar
  20. Choudhary DK, Kasotia A, Jain S, Vaishnav A, Kumari S, Sharma KP, Varma A (2016) Bacterial-mediated tolerance and resistance to plants under abiotic and biotic stresses. J Plant Growth Regul 35:276–300CrossRefGoogle Scholar
  21. Curá JA, Franz DR, Filosofía JE, Balestrasse KB, Burgueño LE (2017) Inoculation with Azospirillum sp. and Herbaspirillum sp. bacteria increases the tolerance of maize to drought stress. Microorganisms 5:1–16Google Scholar
  22. Dixit R, Agrawal L, Gupta S, Kumar M, Yadav S, Chauhan PS, Nautiyal CS (2016) Southern blight disease of tomato control by 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing Paenibacillus lentimorbus B-30488. Plant Signal Behav 11:e1113363CrossRefGoogle Scholar
  23. DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356CrossRefGoogle Scholar
  24. Fan P, Chen D, He Y, Zhou Q, Tian Y, Gao L (2016) Alleviating salt stress in tomato seedlings using Arthrobacter and Bacillus megaterium isolated from the rhizosphere of wild plants grown on saline–alkaline lands. Int J Phytoremediation 18:1113–1121CrossRefGoogle Scholar
  25. Farooq M, Hussain M, Wakeel A, Siddique KHM (2015) Salt stress in maize: effects, resistance mechanisms, and management. A review. Agron Sustain Dev 35:461–481CrossRefGoogle Scholar
  26. Forni C, Duca D, Glick BR (2017) Mechanisms of plant response to salt and drought stress and their alteration by rhizobacteria. Plant Soil 410:335–356CrossRefGoogle Scholar
  27. Fukami J, Cerezini P, Hungria M (2018) Azospirillum: benefits that go far beyond biological nitrogen fixation. AMB Express 8:73CrossRefGoogle Scholar
  28. Gerhardt KE, MacNeill GJ, Gerwing PD, Greenberg BM (2017) Phytoremediation of salt-impacted soils and use of plant growth-promoting rhizobacteria (PGPR) to enhance phytoremediation. In: Ansari AA (ed) Phytoremediation. Springer International Publishing, pp 19–51Google Scholar
  29. Giuffrida F, Scuderi D, Giurato R, Leonardi C (2013) Physiological response of broccoli and cauliflower as affected by NaCl salinity. Acta Hortic 1005:435–441CrossRefGoogle Scholar
  30. Gontia-Mishra I, Sapre S, Kachare S, Tiwari S (2017) Molecular diversity of 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing PGPR from wheat (Triticum aestivum L.) rhizosphere. Plant Soil 414:213–227CrossRefGoogle Scholar
  31. Guo XQ, Li R, Zheng LQ, Lin DQ, Sun JQ, Li SP, Li WJ, Jiang JD (2010) Jeotgalicoccus huakuii sp. nov., a halotolerant bacterium isolated from seaside soil. Int J Syst Evol Microbiol 60:1307–1310CrossRefGoogle Scholar
  32. Gupta S, Kaushal R, Spehia RS, Pathania SS, Sharma V (2017) Productivity of capsicum influenced by conjoint application of isolated indigenous PGPR and chemical fertilizers. J Plant Nutr 40:921–927CrossRefGoogle Scholar
  33. Habib SH, Kausar H, Saud HM (2016) Plant growth-promoting rhizobacteria enhance salinity stress tolerance in okra through ROS-scavenging enzymes. Biomed Res Int 2016:6284547CrossRefGoogle Scholar
  34. Hemeda HM, Klein BP (1990) Effects of naturally occurring antioxidants on peroxidase activity of vegetable extracts. J Food Sci 55:184–185CrossRefGoogle Scholar
  35. Jukes TH, Cantor CR (1969) Evolution of protein molecules. In: Munro HN (ed) Mammalian protein metabolism. Academic, New York, pp 21–132CrossRefGoogle Scholar
  36. Kamjumphol W, Chareonsudjai S, Chareonsudjai P, Wongratanacheewin S, Taweechaisupapong S (2013) Environmental factors affecting Burkholderia pseudomallei biofilm formation. Southeast Asian J Trop Med Public Health 44:72–81Google Scholar
  37. Kang S, Khan AL, Waqas M, You Y, Kim J, Kim J, Hamayun M, Lee I (2014) Plant growth promoting rhizobacteria reduce adverse effects of salinity and osmotic stress by regulating phytohormones and antioxidants in Cucumis sativus. J Plant Interact 9:673–682CrossRefGoogle Scholar
  38. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefGoogle Scholar
  39. Li HQ, Jiang XW (2017) Inoculation with plant growth-promoting bacteria (PGPB) improves salt tolerance of maize seedling. Russ J Plant Physiol 64:235–241CrossRefGoogle Scholar
  40. Li P, Wu J, Qian H (2016) Regulation of secondary soil salinization in semi-arid regions: a simulation research in the Nanshantaizi area along the Silk Road, northwest China. Environ Earth Sci 75:698CrossRefGoogle Scholar
  41. Li H, Lei P, Panga X, Li S, Xu H, Xu Z, Feng X (2017) Enhanced tolerance to salt stress in canola (Brassica napus L.) seedlings inoculated with the halotolerant Enterobacter cloacae HSNJ4. Appl Soil Ecol 119:26–34CrossRefGoogle Scholar
  42. Liu WY, Jiang LL, Guo CJ, Yang SS (2011) Jeotgalicoccus halophilus sp. nov., isolated from salt lakes. Int J Syst Evol Microbiol 61:1720–1724CrossRefGoogle Scholar
  43. Machado RMA, Serralheiro RP (2017) Soil salinity: effect on vegetable crop growth. Management practices to prevent and mitigate soil salinization. Horticulturae 3:30CrossRefGoogle Scholar
  44. Mendis HC, Thomas VP, Schwientek P, Salamzade R, Chien J-T, Waidyarathne P, Kloepper J, Fuente LDL (2018) Strain-specific quantification of root colonization by plant growth promoting rhizobacteria Bacillus firmus I-1582 and Bacillus amyloliquefaciens QST713 in non-sterile soil and field conditions. PLoS One 13:e0193119CrossRefGoogle Scholar
  45. Mishra A, Chauhan PS, Chaudhry V, Tripathi M, Nautiyal CS (2011) Rhizosphere competent Pantoea agglomerans enhances maize (Zea mays) and chickpea (Cicer arietinum L.) growth, without altering the rhizosphere functional diversity. Antonie Van Leeuwenhoek 100:405–413CrossRefGoogle Scholar
  46. Mishra S, Mishra A, Chauhan PS, Mishra SK, Kumari M, Niranjan A, Nautiyal CS (2012) Pseudomonas putida NBRIC19 dihydrolipoamide succinyltransferase (SucB) gene controls degradation of toxic allelochemicals produced by Parthenium hysterophorus. J Appl Microbiol 112:793–808CrossRefGoogle Scholar
  47. Mishra SK, Khan MH, Misra S, Kant VK, Khare P, Srivastava S, Chauhan PS (2017) Characterization of Pseudomonas spp. and Ochrobactrum sp. isolated from volcanic soil. Antonie Van Leeuwenhoek 110:253–270CrossRefGoogle Scholar
  48. Misra S, Dixit VK, Khan MH, Mishra SK, Dviwedi G, Yadav S, Lehri A, Chauhan PS (2017) Exploitation of agro-climatic environment for selection of 1aminocyclopropane-1-carboxylic acid (ACC) deaminase producing salt-tolerant indigenous plant growth promoting rhizobacteria. Microbiol Res 205:25–34CrossRefGoogle Scholar
  49. Mokashe N, Chaudhari A, Patil U (2015) Optimal production and characterization of alkaline protease from newly isolated halotolerant Jeotgalicoccus sp. Biocatal Agric Biotechnol.  https://doi.org/10.1016/j.bcab.2015.01.003
  50. Mukasheva T, Berzhanova R, Ignatova L, Omirbekova A, Brazhnikova Y, Sydykbekova R, Shigaeva M (2016) Bacterial endophytes of Trans-Ili Alatau region’s plants as promising components of a microbial preparation for agricultural use. Acta Biochim Pol 63:321–328CrossRefGoogle Scholar
  51. Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar
  52. Nautiyal CS (1997) A method for selection and characterization of rhizosphere-competent bacteria of chickpea. Curr Microbiol 34:12–17CrossRefGoogle Scholar
  53. Nautiyal CS (1999) An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol Lett 170:265–270CrossRefGoogle Scholar
  54. Nautiyal CS, Srivastava S, Chauhan PS, Seem K, Mishra A, Sopory SK (2013) Plant growth-promoting bacteria Bacillus amyloliquefaciens NBRISN13 modulates gene expression profile of leaf and rhizosphere community in rice during salt stress. Plant Physiol Biochem 66:1–9CrossRefGoogle Scholar
  55. Nazir Q, Akhtar MJ, Imran M, Arshad M, Hussain A, Mahmood S, Hussain S (2017) Simultaneous use of plant growth promoting rhizobacterium and nitrogenous fertilizers may help in promoting growth, yield, and nutritional quality of okra. J Plant Nutr 40:1339–1350CrossRefGoogle Scholar
  56. Negrão S, Schmöckel SM, Tester M (2017) Evaluating physiological responses of plants to salinity stress. Ann Bot 119:1–11CrossRefGoogle Scholar
  57. Patra HK, Mishra M (1979) Pyrophosphatase, peroxidase and polyphenol oxidase activities during leaf development and senescence. Plant Physiol 63:318–323CrossRefGoogle Scholar
  58. Paul D, Nair S (2008) Stress adaptations in a plant growth promoting rhizobacterium (PGPR) with increasing salinity in the coastal agricultural soils. J Basic Microbiol 48:378–384CrossRefGoogle Scholar
  59. Penrose DM, Glick BR (2003) Methods for isolating and characterizing ACC deaminase containing plant growth promoting rhizobacteria. Physiol Plant 118:10–15CrossRefGoogle Scholar
  60. Philips J, Rabaey K, Lovley DR, Vargas M (2017) Biofilm formation by Clostridium ljungdahlii is induced by sodium chloride stress: experimental evaluation and transcriptome analysis. PLoS One 12:e0170406CrossRefGoogle Scholar
  61. Priester JH, Olson SG, Webb SM, Neu MP, Hersman LE, Holden PA (2006) Enhanced exopolymer production and chromium stabilization in Pseudomonas putida unsaturated biofilms. Appl Environ Microbiol 72:1988–1996CrossRefGoogle Scholar
  62. Pumirat P, Vanaporn M, Boonyuen U, Indrawattana N, Rungruengkitkun A, Chantratita N (2017) Effects of sodium chloride on heat resistance, oxidative susceptibility, motility, biofilm and plaque formation of Burkholderia pseudomallei. Microbiology Open 6:e493CrossRefGoogle Scholar
  63. Rengasamy P (2010) Soil processes affecting crop production in salt-affected soils. Aust J Soil Res 37:613–620Google Scholar
  64. Rubio MB, Hermosa R, Vicente R, Gómez-Acosta FA, Morcuende R, Monte E, Bettiol W (2017) The combination of Trichoderma harzianum and chemical fertilization leads to the deregulation of phytohormone networking, preventing the adaptive responses of tomato plants to salt stress. Front Plant Sci 8:294CrossRefGoogle Scholar
  65. Sadeghi A, Karimi E, Dahaji PA, Javid MG, Dalvand Y, Askari H (2012) Plant growth promoting activity of an auxin and siderophore producing isolate of Streptomyces under saline soil conditions. World J Microbiol Biotechnol 28:1503–1509CrossRefGoogle Scholar
  66. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
  67. Sandhya V, Ali SZ (2015) The production of exopolysaccharide by Pseudomonas putida GAP-P45 under various abiotic stress conditions and its role in soil aggregation. Microbiology 84:512–519CrossRefGoogle Scholar
  68. Sandhya V, Ali SKZ, Minakshi G, Gopal R, Venkateswarlu B (2009) Alleviation of drought stress effects in sunflower seedlings by the exopolysaccharides producing Pseudomonas putida strain GAPP45. Biol Fertil Soils 46:17–26CrossRefGoogle Scholar
  69. Sarkar A, Ghosh PK, Pramanik K, Mitra S, Soren T, Pandey S, Mondal MH, Maiti TK (2017) A halotolerant Enterobacter sp. displaying ACC deaminase activity promotes rice seedling growth under salt stress. Res Microbiol.  https://doi.org/10.1016/j.resmic.2017.08.005
  70. Sharma A, Singh P, Kumar S, Kashyap PL, Srivastava AK, Chakdar H, Singh RN, Kaushik R, Saxena AK, Sharma AK (2015) Deciphering diversity of salt-tolerant bacilli from saline soils of eastern Indo-Gangetic plains of India. Geomicrobiol J 32:170–180CrossRefGoogle Scholar
  71. Sheng GP, Yu HQ, Yue Z (2006) Factors influencing the production of extracellular polymeric substances by Rhodopseudomonas acidophila. Int Biodeter Biodegr 58:89–93CrossRefGoogle Scholar
  72. Siddikee MA, Sundaram S, Chandrasekaran M, Kim K, Selvakumar G, Sa T (2015) Halotolerant bacteria with ACC deaminase activity alleviate salt stress effect in canola seed germination. J Korean Soc Appl Biol Chem 58:237–241CrossRefGoogle Scholar
  73. Singh RP, Jha PN (2016) The multifarious PGPR Serratia marcescens CDP-13 augments induced systemic resistance and enhanced salinity tolerance of wheat (Triticum aestivum L.). PLoS One 11:e0155026CrossRefGoogle Scholar
  74. Soleimani R, Alikhani HA, Towfighi H, Khavazi K, Pourbabaee AA (2017) Isolated bacteria from saline-sodic soils alter the response of wheat under high adsorbed sodium and salt stress. Int J Environ Sci Technol 14:143–150CrossRefGoogle Scholar
  75. Sood G, Kaushal R, Chauhan A, Gupta S (2018) Effect of conjoint application of indigenous PGPR and chemical fertilizers on productivity of maize (Zea mays L.) under mid hills of Himachal Pradesh. J Plant Nutr 41:297–303CrossRefGoogle Scholar
  76. Srivastava S, Yadav A, Seem K, Mishra S, Chaudhary V, Nautiyal CS (2008) Effect of high temperature on Pseudomonas putida NBRI0987 biofilm formation and expression of stress sigma factor RpoS. Curr Microbiol 56:453–457CrossRefGoogle Scholar
  77. Suarez C, Cardinale M, Ratering S, Steffens D, Jung S, Montoya AMZ, Geissler-Plaum R, Schnell S (2015) Plant growth-promoting effects of Hartmannibacter diazotrophicus on summer barley (Hordeum vulgare L.) under salt stress. Appl Soil Ecol 95:23–30CrossRefGoogle Scholar
  78. Tank N, Saraf M (2010) Salinity-resistant plant growth promoting rhizobacteria ameliorates sodium chloride stress on tomato plants. J Plant Interact 5:51–58CrossRefGoogle Scholar
  79. Titus S, Gasnkar N, Srivastava KB, Karande AA (1995) Exopolymer production by a fouling marine bacterium Pseudomonas alcaligenes. Indian J Mar Sci 24:45–48Google Scholar
  80. Tiwari S, Lata C, Chauhan PS, Nautiyal CS (2016) Pseudomonas putida attunes morphophysiological, biochemical and molecular responses in Cicer arietinum L. during drought stress and recovery. Plant Physiol Biochem 99:108–117CrossRefGoogle Scholar
  81. Ullah S, Bano A (2015) Isolation of plant-growth-promoting rhizobacteria from rhizospheric soil of halophytes and their impact on maize (Zea mays L.) under induced soil salinity. Can J Microbiol 61:307–313CrossRefGoogle Scholar
  82. Upadhyay SK, Singh JS, Singh DP (2011) Exopolysaccharide-producing plant growth-promoting rhizobacteria under salinity condition. Pedosphere 21:214–222CrossRefGoogle Scholar
  83. Vaishnav A, Varma A, Tuteja N, Choudhary DK (2016) PGPR-mediated amelioration of crops under salt stress. In: Choudhary DK (ed) Plant-microbe interaction: an approach to sustainable agriculture. Springer Nature Pte Ltd., Singapore, pp 205–226CrossRefGoogle Scholar
  84. Vardharajula S, Ali SZ, Grover M, Reddy G, Bandi V (2011) Drought-tolerant plant growth promoting Bacillus spp.: effect on growth, osmolytes, and antioxidant status of maize under drought stress. J Plant Interact 6:1–14CrossRefGoogle Scholar
  85. Vurukonda SSKP, Vardharajula S, Shrivastava M, Ali SKZ (2016) Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiol Res 184:13–24CrossRefGoogle Scholar
  86. Yadav AN, Sachan SG, Verma P, Saxena AK (2016) Bioprospecting of plant growth promoting psychrotrophic bacilli from the cold dessert of north western Indian Himalayas. Indian J Exp Biol 54:142–150Google Scholar
  87. Yasmin H, Nosheen A, Naz R, Bano A, Keyani R (2017) L-tryptophan-assisted PGPR-mediated induction of drought tolerance in maize (Zea mays L.). J Plant Interact 12:567–578CrossRefGoogle Scholar

Copyright information

© Università degli studi di Milano 2019

Authors and Affiliations

  1. 1.Microbial Technologies DivisionCouncil of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI)LucknowIndia
  2. 2.Academy of Scientific &Innovative ResearchChennaiIndia

Personalised recommendations