Demonstrating the potential of abiotic stress-tolerant Jeotgalicoccus huakuii NBRI 13E for plant growth promotion and salt stress amelioration
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The present study aimed to demonstrate the potential of abiotic stress-tolerant Jeotgalicoccus huakuii NBRI 13E for plant growth promotion and salt stress amelioration. NBRI 13E was characterized for abiotic stress tolerance and plant growth-promoting (PGP) attributes under normal and salt stress conditions. Phylogenetic comparison of NBRI 13E was carried out with known species of the same genera based on 16S rRNA gene. Plant growth promotion and rhizosphere colonization studies were determined under greenhouse conditions using maize, tomato, and okra. Field experiment was also performed to assess the ability of NBRI 13E inoculation for improving growth and yield of maize crop in alkaline soil. NBRI 13E demonstrated abiotic stress tolerance and different PGP attributes under in vitro conditions. Phylogenetic and differential physiological analysis revealed considerable differences in NBRI 13E as compared with the reported species for Jeotgalicoccus genus. NBRI 13E colonizes in the rhizosphere of the tested crops, enhances plant growth, and ameliorates salt stress in a greenhouse experiment. Modulation in defense enzymes, chlorophyll, proline, and soluble sugar content in NBRI 13E-inoculated plants leads to mitigate the deleterious effect of salt stress. Furthermore, field evaluation of NBRI 13E inoculation using maize was carried out with recommended 50 and 100% chemical fertilizer controls, which resulted in significant enhancement of all vegetative parameters and total yield as compared to respective controls. Jeotgalicoccus huakuii NBRI 13E is reported for the first time for its ability to develop a bioinoculant formulation for stress amelioration and improved crop productivity.
KeywordsAbiotic stress Defense enzymes Jeotgalicoccus huakuii Maize PGP attributes
The authors acknowledge the Director, CSIR-National Botanical Research Institute for providing facilities and support during the study.
The authors acknowledge the financial assistance from the CSIR Network project MLP022 and In-house project OLP105.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
- AVRDC (2006) Vegetables matter. In: AVRDC – The World Vegetable Center. Shanhua, TaiwanGoogle Scholar
- Bharti N, Yadav D, Barnawal D, Maji D, Kalra A (2013) Exiguobacterium oxidotolerans, a halotolerant plant growth promoting rhizobacteria, improves yield and content of secondary metabolites in Bacopa monnieri (L.) Pennell under primary and secondary salt stress. World J Microbiol Biotechnol 29:379–387CrossRefGoogle Scholar
- Bistgani ZE, Siadat SA, Bakhshandeh A, Pirbalouti AG, Hashemi M (2017) Interactive effects of drought stress and chitosan application on physiological characteristics and essential oil yield of Thymus daenensis Celak. Crop J. https://doi.org/10.1016/j.cj.2017.04.00
- Bric JM, Bostock RM, Silverstone SE (1991) Rapid in situ assay for indole acetic acid production by bacteria immobilized on a nitrocellulose membrane. Appl Environ Microbiol 57:535–538Google Scholar
- Chen S, Xing J, Lan H (2012) Comparative effects of neutral salt and alkaline salt stress on seed germination, early seedling growth and physiological response of a halophyte species Chenopodium glaucum. Afr J Biotechnol 11:9572–9581Google Scholar
- Curá JA, Franz DR, Filosofía JE, Balestrasse KB, Burgueño LE (2017) Inoculation with Azospirillum sp. and Herbaspirillum sp. bacteria increases the tolerance of maize to drought stress. Microorganisms 5:1–16Google Scholar
- Gerhardt KE, MacNeill GJ, Gerwing PD, Greenberg BM (2017) Phytoremediation of salt-impacted soils and use of plant growth-promoting rhizobacteria (PGPR) to enhance phytoremediation. In: Ansari AA (ed) Phytoremediation. Springer International Publishing, pp 19–51Google Scholar
- Kamjumphol W, Chareonsudjai S, Chareonsudjai P, Wongratanacheewin S, Taweechaisupapong S (2013) Environmental factors affecting Burkholderia pseudomallei biofilm formation. Southeast Asian J Trop Med Public Health 44:72–81Google Scholar
- Mendis HC, Thomas VP, Schwientek P, Salamzade R, Chien J-T, Waidyarathne P, Kloepper J, Fuente LDL (2018) Strain-specific quantification of root colonization by plant growth promoting rhizobacteria Bacillus firmus I-1582 and Bacillus amyloliquefaciens QST713 in non-sterile soil and field conditions. PLoS One 13:e0193119CrossRefGoogle Scholar
- Misra S, Dixit VK, Khan MH, Mishra SK, Dviwedi G, Yadav S, Lehri A, Chauhan PS (2017) Exploitation of agro-climatic environment for selection of 1aminocyclopropane-1-carboxylic acid (ACC) deaminase producing salt-tolerant indigenous plant growth promoting rhizobacteria. Microbiol Res 205:25–34CrossRefGoogle Scholar
- Mokashe N, Chaudhari A, Patil U (2015) Optimal production and characterization of alkaline protease from newly isolated halotolerant Jeotgalicoccus sp. Biocatal Agric Biotechnol. https://doi.org/10.1016/j.bcab.2015.01.003
- Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar
- Rengasamy P (2010) Soil processes affecting crop production in salt-affected soils. Aust J Soil Res 37:613–620Google Scholar
- Rubio MB, Hermosa R, Vicente R, Gómez-Acosta FA, Morcuende R, Monte E, Bettiol W (2017) The combination of Trichoderma harzianum and chemical fertilization leads to the deregulation of phytohormone networking, preventing the adaptive responses of tomato plants to salt stress. Front Plant Sci 8:294CrossRefGoogle Scholar
- Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
- Sarkar A, Ghosh PK, Pramanik K, Mitra S, Soren T, Pandey S, Mondal MH, Maiti TK (2017) A halotolerant Enterobacter sp. displaying ACC deaminase activity promotes rice seedling growth under salt stress. Res Microbiol. https://doi.org/10.1016/j.resmic.2017.08.005
- Titus S, Gasnkar N, Srivastava KB, Karande AA (1995) Exopolymer production by a fouling marine bacterium Pseudomonas alcaligenes. Indian J Mar Sci 24:45–48Google Scholar
- Yadav AN, Sachan SG, Verma P, Saxena AK (2016) Bioprospecting of plant growth promoting psychrotrophic bacilli from the cold dessert of north western Indian Himalayas. Indian J Exp Biol 54:142–150Google Scholar