Annals of Microbiology

, Volume 69, Issue 3, pp 299–305 | Cite as

Role of Pseudomonas aeruginosa lipopolysaccharides in modulation of biofilm and virulence factors of Enterobacteriaceae

  • Shaymaa H. Abdel-RhmanEmail author
Original Article


Enterobacteriaceae members are largely distributed in the environment and responsible for a wide range of bacterial infections in hospitalized patients. Pseudomonas aeruginosa (P. aeruginosa) causes severe nosocomial infections associated with severe inflammation due to its potent virulent factors including lipopolysaccharide (LPS). The aim of this study is to assess the bacterial LPS effect on Enterobacteriaceae biofilm and other virulence factors in vitro. The effect of P. aeruginosa LPS on biofilm formation of two other species of Enterobacteriaceae (Escherichia coli and Klebsiella pneumoniae) was assessed using a standard biofilm assay. PCR was performed on genes of biofilm and virulence factors. Expression of biofilm, type-1-fimbriae and serum resistance genes in treated and untreated cells was measured with RT-PCR. P. aeruginosa LPS has the ability to stimulate biofilm formation and stabilize the already formed biofilm significantly in all tested strains. In addition, LPS significantly increased the level of expression of Bss, FimH, and Iss genes when measured by RT-PCR. P. aeruginosa LPS has a direct stimulatory effect on the biofilm formation, type-1-fimbriae, and serum resistance in both E. coli and K. pneumoniae. So, the presence of P. aeruginosa in mixed infection with Enterobactereacea leads to increase their virulence.


P. aeruginosa LPS E. coli K. pneumoniae Biofilm Virulence factors 



All thanks and appreciation to Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, N1G 2W1, Canada, for providing the standard strains used in this study. This work was performed at the Microbiology Department, Faculty of Pharmacy, Mansoura University, Egypt and Department of Pharmaceutics and Pharmaceutical Biotechnology, Faculty of Pharmacy, Taibah University, AlMadinah Al Munawwarah, Kingdom of Saudi Arabia.

Compliance with ethical standards

Conflict of interest

The author declares that there is no conflict of interest.


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Copyright information

© Università degli studi di Milano 2018

Authors and Affiliations

  1. 1.Department of Pharmaceutics and Pharmaceutical Biotechnology, Faculty of PharmacyTaibah UniversityAlMadinah Al MunawwarahKingdom of Saudi Arabia
  2. 2.Department of Microbiology and Immunology, Faculty of PharmacyMansoura UniversityMansouraEgypt

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